Flow cytometric investigation of heterogeneous copper-sensitivity in asynchronously grown Saccharomyces cerevisiae.

abstract：:Cell wall LPS of Escherichia coli are organized as particles which are visible in the electron microscope, after treatment of the wall with alkali. We now describe alkali treated walls of three E. coli strains with differences in susceptibility to the T4 phage infection. Strain CR63, a usual host for the T4 phage, sho...

journal_title：FEMS microbiology letters

authors： Zorzopulos J,de Long S,Chapman V,Kozloff LM

更新日期：1989-10-01 00:00:00

abstract：:It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

journal_title：FEMS microbiology letters

authors： Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

更新日期：2007-09-01 00:00:00

abstract：:Myxococcus xanthus cells move over surfaces by gliding motility. The frz signal transduction system is used to control the reversal frequency, and thus the overall direction of movement of M. xanthus cells. We analyzed the behavior of wild-type and frz mutant cells in response to prey bacteria (Escherichia coli). Wild...

journal_title：FEMS microbiology letters

authors： McBride MJ,Zusman DR

更新日期：1996-04-01 00:00:00

abstract：:The capsular polysaccharide released from the bacterial surface by cell wall turnover during growth exhibited less size heterogeneity and a higher average molecular mass than the polysaccharide extracted from the cell by treatment with lysostaphin or low pH. Treatment of turnover polysaccharide, radiolabelled by growt...

journal_title：FEMS microbiology letters

authors： Havaei SA,Hancock IC

更新日期：1994-05-01 00:00:00

abstract：:We transformed a clinical Staphylococcus epidermidis isolate with the Enterococcus faecalis transposon Tn917-carrying plasmid pTV1. Loss of plasmid replication was observed at 47 degrees C. Tn917 transposes efficiently and apparently randomly. The transposition frequency could be stimulated by erythromycin. Transposon...

journal_title：FEMS microbiology letters

authors： Grueter L,Koenig O,Laufs R

更新日期：1991-08-01 00:00:00

abstract：:Synthetic oligonucleotide primers were used in a polymerase chain reaction (PCR) protocol to target a specific sequence in the gene coding for the A subunit of Escherichia coli verotoxin (VTe-variant, VTev). This PCR protocol permits the VTe-variant target sequence to be distinguished from closely related sequences in...

journal_title：FEMS microbiology letters

authors： Johnson WM,Tyler SD,Wang G,Lior H

更新日期：1991-11-15 00:00:00

abstract：:The commercialization of the n-butanol bioprocess is largely dependent on the price of feedstocks. Renewable cellulose appears to be an appealing feedstock. The microbial production of n-butanol still remains challenging because of the limited availability of intracellular NADH. To address this issue, an Escherichia c...

journal_title：FEMS microbiology letters

authors： Saini M,Chiang CJ,Li SY,Chao YP

更新日期：2016-02-01 00:00:00

abstract：:The insertion sequence ISRm8 was identified by sequence analysis of the cryptic plasmid pRmeGR4b of Sinorhizobium meliloti GR4. ISRm8 is 1451 bp in length and carries 22/24-bp terminal imperfect inverted repeats with seven mismatches and a direct target site duplication of 3 bp. ISRm8 carries a unique open reading fra...

journal_title：FEMS microbiology letters

authors： Zekri S,Toro N

更新日期：1998-01-01 00:00:00

abstract：:A number of enteric pathogens, including enteropathogenic (EPEC) and enterohemorrhagic (EHEC) Escherichia coli, Hafnia alvei, a strain of Citrobacter freundii, and rabbit EPEC strain RDEC-1 cause attaching-effacing (AE) lesions in the gut mucosa. These bacteria have a pathogenicity cassette (locus of enterocyte efface...

journal_title：FEMS microbiology letters

authors： Agin TS,Cantey JR,Boedeker EC,Wolf MK

更新日期：1996-11-01 00:00:00

abstract：:An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, ...

journal_title：FEMS microbiology letters

authors： Jiang H,Kendrick KE

更新日期：2000-12-01 00:00:00

abstract：:Escherichia coli has been used widely in laboratory and the biotech industry. However, the genetic and metabolic characteristics remain inadequately studied, particularly for those strains with extensive genetic manipulations that might have resulted in unknown mutations. Here, we demonstrate a comparative proteomics ...

journal_title：FEMS microbiology letters

authors： Xia XX,Qian ZG,Lee SY

更新日期：2011-01-01 00:00:00

abstract：:While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammon...

journal_title：FEMS microbiology letters

authors： Alzerreca JJ,Norton JM,Klotz MG

更新日期：1999-11-01 00:00:00

abstract：:Pectinases are enzymes that catalyze pectin degradation. There is a global demand for pectinases because of their wide utility and catalytic efficiency. Optimization of the fermentation process to increase the pectolytic enzyme activity is generally practiced to lower process costs, but whether temperature influences ...

journal_title：FEMS microbiology letters

authors： Guan Y,Yin D,Du X,Ye X

更新日期：2018-12-01 00:00:00

abstract：:A Ca(2+)/calmodulin-dependent protein kinase (CaMK) gene was cloned and characterized from Arthrobotrys dactyloides, a nematode-trapping fungus. The resulting 373-amino-acid protein, FCaMK, has significant homology to mammalian CaMKs. FCaMK contains a serine/threonine kinase domain followed by a calmodulin-binding dom...

journal_title：FEMS microbiology letters

authors： Tsai PJ,Tu J,Chen TH

更新日期：2002-06-18 00:00:00

abstract：:Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...

journal_title：FEMS microbiology letters

authors： Yamamura H,Hanawa K,Kusunoki M,Nakamura K,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Fujita N,Hayakawa M

更新日期：2011-11-01 00:00:00

abstract：:We used error-prone PCR to generate mutations in a subtilisin protease-encoding gene, and screened for recombinants that expressed temperature-sensitive (TS) variants. From the dozens of mutations that we detected in the recombinant genes we found that those mutations that affected aspartate-75 had the most profound e...

journal_title：FEMS microbiology letters

authors： Thompson VC,McGuire BE,Frier MS,Legg MSG,Dyer TW,Gudavicius G,Potter S,Nano FE

更新日期：2020-10-16 00:00:00

abstract：:Streptomyces coelicolor A3(2) produces an antibiotic, actinorhodin, which belongs to the aromatic polyketides and which can function as an acid/base indicator. Its production results in the death of microorganisms in the vicinity of S. coelicolor A3(2), and this phenomenon can be used in concert with biopesticides. Th...

journal_title：FEMS microbiology letters

authors： Lee Y,Kim K,Suh JW,Rhee S,Lim Y

更新日期：2007-01-01 00:00:00

abstract：:The miniplasmid profiles of 18 Bacillus thuringiensis strains belonging to 8 different serotypes were determined using an alkaline hydrolysis method for isolation of low molecular mass plasmids. Nearly all the strains contained covalently closed circular (CCC) DNA species ranging from 2 to 5 species per strain and fro...

journal_title：FEMS microbiology letters

authors： Marin R,Tanguay RM,Valéro J,Letarte R,Bellemare G

更新日期：1992-07-15 00:00:00

abstract：:Ammonium transporters form a conserved family of transport proteins and are widely distributed among all domains of life. The genome of Nitrosomonas europaea codes for a single gene (rh1) that belongs to the family of the AMT/Rh ammonium transporters. For the first time, this study provides functional and physiologica...

journal_title：FEMS microbiology letters

authors： Weidinger K,Neuhäuser B,Gilch S,Ludewig U,Meyer O,Schmidt I

更新日期：2007-08-01 00:00:00

abstract：:Bifidobacteria are normal inhabitants of the human gut, and members of which are generally considered to be probiotic. Before exerting their beneficial properties, they must survive and persist in the physiological concentrations (0.05-2%) of bile in the gut. In this work, the functional role of tlyC1 encoding a hemol...

journal_title：FEMS microbiology letters

authors： Liu Y,An H,Zhang J,Zhou H,Ren F,Hao Y

更新日期：2014-11-01 00:00:00

abstract：:The virulence of Salmonella typhimurium for mice is dependent on a plasmid-borne gene cluster termed spv. We previously determined that both S. typhimurium and Escherichia coli bacteria grown in a rich medium preferentially express the spv genes during the stationary phase of growth. In this study we evaluated the rol...

journal_title：FEMS microbiology letters

authors： Norel F,Robbe-Saule V,Popoff MY,Coynault C

更新日期：1992-12-01 00:00:00

abstract：:The photoinduced bactericidal capacity of TiO(2) based films was evaluated, using as model organism Pseudomonas aeruginosa. Thin films were obtained by spray pyrolysis; they included undoped, Cu doped, and Al doped TiO(2). Scanning electron microscopy was used to observe the final effect of the irradiated films upon t...

journal_title：FEMS microbiology letters

authors： Amézaga-Madrid P,Nevárez-Moorillón GV,Orrantia-Borunda E,Miki-Yoshida M

更新日期：2002-06-04 00:00:00

abstract：:The activities of some enzymes belonging to the Leloir pathway, phosphoglucomutase, UDP-glucose pyrophosphorylase, UDP-galactose 4-epimerase and galactose 1-P uridyl transferase, were studied in a wild ropy, a non-ropy and an overproducing mutant ropy strain of Streptococcus thermophilus. These activities were assayed...

journal_title：FEMS microbiology letters

authors： Escalante A,Villegas J,Wacher C,García-Garibay M,Farrés A

更新日期：2002-04-09 00:00:00

abstract：:The Staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and an SH3b cell wall-binding domain. In turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis pathogens, Staphylococcus aureus and coagulase-negative staphy...

journal_title：FEMS microbiology letters

authors： Donovan DM,Lardeo M,Foster-Frey J

更新日期：2006-12-01 00:00:00

abstract：:The sequences of two Piromyces cDNAs (manB and manC) encoding functional mannanases, defined as mannanase B (MANB) and mannanase C (MANC), revealed that both the cDNAs, and the encoded enzymes, exhibited extensive sequence identity with each other and with a previously described Piromyces mannanase. MANB and MANC, whi...

journal_title：FEMS microbiology letters

authors： Millward-Sadler SJ,Hall J,Black GW,Hazlewood GP,Gilbert HJ

更新日期：1996-08-01 00:00:00

abstract：:We have studied the single rRNA gene cluster from the Archaeon, Pyrococcus furiosus. This isolate grows optimally at 100 degrees C and is thus a hyperthermophile. In P. furiosus, transcription of 16S rRNA is subject to regulation over a 7.5-fold range in response to a 20-fold increase in growth rate. The single cluste...

journal_title：FEMS microbiology letters

authors： DiRuggiero J,Achenbach LA,Brown SH,Kelly RM,Robb FT

更新日期：1993-08-01 00:00:00

abstract：:Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was expressed with N-terminal fusion of a LacZ-derived secretion motif (TMITNSSSVP) using the lac promoter system in recombinant Escherichia coli JM109 [pUDF-A81]. In flask cultures, recombinant enzyme activity was detected in culture media, and sequ...

journal_title：FEMS microbiology letters

authors： Lee J,Saraswat V,Koh I,Song KB,Park YH,Rhee SK

更新日期：2001-02-20 00:00:00

abstract：:The microbial adhesion to hydrocarbons (MATH) test is one of the most common method to determine the hydrophobicity of cell surfaces. Despite its prevalence, no standard test parameters are used in literature, making a direct comparison of data almost impossible. Criticism also focuses on test parameters that may mask...

journal_title：FEMS microbiology letters

authors： Nachtigall C,Weber C,Rothenburger S,Jaros D,Rohm H

更新日期：2019-06-01 00:00:00

abstract：:Within the phylum Bacteroidetes, the gyrB gene, encoding for the B subunit of the DNA gyrase, has been used as a phylogenetic marker for several genera closely related to Flavobacterium. The phylogenies of the complete 16S rRNA gene and the gyrB gene were compared for 33 Antarctic Flavobacterium isolates and 23 type s...

journal_title：FEMS microbiology letters

authors： Peeters K,Willems A

更新日期：2011-08-01 00:00:00

abstract：:Bacteriophage P008 revealed irreversible and uniform adsorption to cell walls of L. lactis subsp. 'diacetylactis' F7/2, whereas phage P127 adsorbed reversibly to a limited number of receptor sites on cell walls of L. lactis subsp. cremoris Wg2-1. Neither extraction of lipids, cell wall- and membrane-teichoic acids nor...

journal_title：FEMS microbiology letters

authors： Schäfer A,Geis A,Neve H,Teuber M

更新日期：1991-02-01 00:00:00