Cloning and characterization of the gene encoding penicillin-binding protein A of Streptomyces griseus.

abstract：:The majority of microorganisms in natural environments are difficult to cultivate, but their genes can be studied via metagenome libraries. To enhance the chances that these genes become expressed we here report the construction of a broad-host-range plasmid vector (pRS44) for fosmid and bacterial artificial chromosom...

journal_title：FEMS microbiology letters

authors： Aakvik T,Degnes KF,Dahlsrud R,Schmidt F,Dam R,Yu L,Völker U,Ellingsen TE,Valla S

更新日期：2009-06-01 00:00:00

abstract：:This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

journal_title：FEMS microbiology letters

authors： Nastos S,Rangachari PK

更新日期：2016-08-01 00:00:00

abstract：:Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

journal_title：FEMS microbiology letters

authors： Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

更新日期：2004-09-15 00:00:00

abstract：:The riboflavin kinase encoding gene ribR is situated within a 12 genes locus ytmI-ytnM of the Bacillus subtilis chromosome. Here we demonstrate that ribR is transcribed as part of a 10 kb ytmI-ytnM operon. The riboflavin overproduction phenotype of B. subtilis ribC mutant strains, which is a result of the strongly red...

journal_title：FEMS microbiology letters

authors： Solovieva IM,Kreneva RA,Errais Lopes L,Perumov DA

更新日期：2005-02-01 00:00:00

abstract：:We present evidence that glycine betaine (betaine) which was synthesized from choline was excreted and reaccumulated in osmoregulating cells of Escherichia coli. Choline which was accumulated in bet mutants defective in betaine synthesis was shown to be excreted in response to betaine uptake. Our data suggest that E. ...

journal_title：FEMS microbiology letters

authors： Lamark T,Styrvold OB,Strøm AR

更新日期：1992-09-15 00:00:00

abstract：:Recent reports have indicated that cholesterol plays a crucial role during the uptake of mycobacteria by macrophages. However, the significance of cholesterol modification enzymes encoded by Mycobacterium tuberculosis for bacterial pathogenicity remains unknown. Here, the authors explored whether the well-known choles...

journal_title：FEMS microbiology letters

authors： Brzostek A,Dziadek B,Rumijowska-Galewicz A,Pawelczyk J,Dziadek J

更新日期：2007-10-01 00:00:00

abstract：:Helicobacter pylori possesses a gene (HP0326/JHP309) homologous to neuA of other bacteria, encoding a cytidyl monophosphate-N-acetylneuraminic acid synthetase-homologous enzyme in its N-terminal portion. We analysed the function of this gene, which is controlled by a flagellar class 2 sigma(54) promoter, in flagellar ...

journal_title：FEMS microbiology letters

authors： Josenhans C,Vossebein L,Friedrich S,Suerbaum S

更新日期：2002-05-07 00:00:00

abstract：:Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine fece...

journal_title：FEMS microbiology letters

authors： Vuddhakul V,Patararungrong N,Pungrasamee P,Jitsurong S,Morigaki T,Asai N,Nishibuchi M

更新日期：2000-01-15 00:00:00

abstract：:The plant-growth promoting rhizobacterium Azospirillum lipoferum strain 4B generates in vitro a stable phase variant designated 4VI at frequencies of 10(-4) to 10(-3) per cell per generation. Variant 4VI displays pleitropic modifications, such as the loss of swimming motility and the inability to assimilate certain su...

journal_title：FEMS microbiology letters

authors： Vial L,Pothier JF,Normand P,Moënne-Loccoz Y,Bally R,Wisniewski-Dyé F

更新日期：2004-07-15 00:00:00

abstract：:In a large-scale gene disruption screen of Magnaporthe oryzae, a gene MoST1 encoding a protein belonging to the hexose transporter family was identified as a gene required for conidiation and culture pigmentation. The gene MoST1 located on chromosome V of the M. oryzae genome was predicted to be 1892 bp in length with...

journal_title：FEMS microbiology letters

authors： Saitoh H,Hirabuchi A,Fujisawa S,Mitsuoka C,Terauchi R,Takano Y

更新日期：2014-03-01 00:00:00

abstract：:Mycobacterium sp. PYR-1 was previously isolated in our laboratory and was shown to be able to mineralize high molecular mass polycyclic aromatic hydrocarbons (PAHs) [Heitkamp and Cerniglia, (1988) Appl. Environ. Microbiol. 54, 1612-1614]. In this research, the 16S rRNA gene (rDNA) of this strain was amplified by polym...

journal_title：FEMS microbiology letters

authors： Wang RF,Cao WW,Cerniglia CE

更新日期：1995-07-15 00:00:00

abstract：:The copZ gene of Bacillus subtilis encodes a copper chaperon CopZ that donates copper to the copper transporter CopA. Both genes copZ and copA are clustered to an operon and its promoter is regulated by Cu ions and CueR, a Mer-like transcriptional activator. Here we show that cadmium ions activate copZA expression as ...

journal_title：FEMS microbiology letters

authors： Solovieva IM,Entian KD

更新日期：2004-07-01 00:00:00

abstract：:Five independent collections, comprising a total of 34 clones encoding cellulases, hemicellulases and cell surface proteins of Clostridium thermocellum, were searched for overlapping or contiguous DNA fragments. The clones were hybridized to large genomic restriction fragments separated by pulse-field electrophoresis....

journal_title：FEMS microbiology letters

authors： Guglielmi G,Béguin P

更新日期：1998-04-01 00:00:00

abstract：:DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

journal_title：FEMS microbiology letters

authors： Gowher H,Ehrlich KC,Jeltsch A

更新日期：2001-11-27 00:00:00

abstract：:Mapping of the plasmid-encoded RNA of the intracellular parasite, Chlamydia trachomatis revealed that the upstream control elements are different from those of other Gram-negative bacteria. A tetranucleotide, AYAA was found near the -10 position, in 5 out of 8 upstream sequences described so far. The plasmid also has ...

journal_title：FEMS microbiology letters

authors： Sriprakash KS,Pearce BJ

更新日期：1990-09-15 00:00:00

abstract：:The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles...

journal_title：FEMS microbiology letters

authors： Pérez MA,Gallego FJ,Hidalgo P

更新日期：2001-12-18 00:00:00

abstract：:Acinetobacter baumannii strain A148, a clinical isolate resistant to imipenem (MIC = 32 mg l-1), synthesized two beta-lactamases with pIs 6.3 and > 9.2. The pI 6.3 enzyme hydrolyzed the penicillins, including isoxazoylpenicillins, first-, second- and, to a lesser extent, third-generation cephalosporins. It was inhibit...

journal_title：FEMS microbiology letters

authors： Hornstein M,Sautjeau-Rostoker C,Péduzzi J,Vessières A,Hong LT,Barthélémy M,Scavizzi M,Labia R

更新日期：1997-08-15 00:00:00

abstract：:The beta-lactamase genes blaA and blaB were detected by PCR amplification in strains of Yersinia enterocolitica biovar 1A isolated from India, Germany, France and the USA. Both genes were detected in all strains. Polymerase chain reaction-restriction fragment length polymorphism revealed genetic heterogeneity in blaA ...

journal_title：FEMS microbiology letters

authors： Sharma S,Mittal S,Mallik S,Virdi JS

更新日期：2006-04-01 00:00:00

abstract：:Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. Howeve...

journal_title：FEMS microbiology letters

authors： Morton DJ,Madore LL,Smith A,Vanwagoner TM,Seale TW,Whitby PW,Stull TL

更新日期：2005-12-15 00:00:00

abstract：:Chagas disease, caused by Trypanosoma cruzi, affects six to seven million people worldwide. Treatment is based on benznidazole, producing several side effects and debatable efficacy, highlighting the need for new alternative drugs. We investigated the activity of four C-4 functionalized azlactone derivatives (EPA-27, ...

journal_title：FEMS microbiology letters

authors： de Azeredo CM,Ávila EP,Pinheiro DL,Amarante GW,Soares MJ

更新日期：2017-02-01 00:00:00

abstract：:The activities of some enzymes belonging to the Leloir pathway, phosphoglucomutase, UDP-glucose pyrophosphorylase, UDP-galactose 4-epimerase and galactose 1-P uridyl transferase, were studied in a wild ropy, a non-ropy and an overproducing mutant ropy strain of Streptococcus thermophilus. These activities were assayed...

journal_title：FEMS microbiology letters

authors： Escalante A,Villegas J,Wacher C,García-Garibay M,Farrés A

更新日期：2002-04-09 00:00:00

abstract：:mlrA is the only microcystin-degrading gene detected in Sphingomonas sp. MJ-PV. The gene has an extremely rare nucleotide sequence and homologous genes have not yet been discovered in the DNA database. We discovered the existence of a gene homologous to mlrA in new microcystin-degrading bacteria, MD-1 and Y2. These st...

journal_title：FEMS microbiology letters

authors： Saito T,Okano K,Park HD,Itayama T,Inamori Y,Neilan BA,Burns BP,Sugiura N

更新日期：2003-12-12 00:00:00

abstract：:We have devised a reliable procedure for the separation of three beta-lactamases of isoelectric focusing points (pI), 5.4, 6.5, and 7.9 by Fast Protein Liquid Chromatography (FPLC System). All of these enzymes were transferable and originated from a ceftazidime and cefotaxime resistant Klebsiella pneumoniae isolated i...

journal_title：FEMS microbiology letters

authors： Payne DJ,Hood J,Marriott MS,Amyes SG

更新日期：1990-06-01 00:00:00

abstract：:The filamentous ascomycete Botrytis cinerea is one of the most studied models for understanding the necrotrophic behaviour of phytopathogenic fungi. The genomes of two strains of B. cinerea have been sequenced (B05.10 and T4), which may contribute to elucidating the virulence polymorphism in this fungus. In this study...

journal_title：FEMS microbiology letters

authors： Choquer M,Robin G,Le Pêcheur P,Giraud C,Levis C,Viaud M

更新日期：2008-12-01 00:00:00

abstract：:A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

journal_title：FEMS microbiology letters

authors： You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

更新日期：1996-03-01 00:00:00

abstract：:The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

journal_title：FEMS microbiology letters

authors： Teramoto H,Tanaka H,Wariishi H

更新日期：2004-05-15 00:00:00

abstract：:Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...

journal_title：FEMS microbiology letters

authors： Bricker AL,Camilli A

更新日期：1999-03-15 00:00:00

abstract：:A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. Sequence analysis revealed that this cluster contains the genes for the scaffolding protein CipA, the processive endocellulase Cel48A, several endoglucanases of families 5 and 9, the mannanase Man5...

journal_title：FEMS microbiology letters

authors： Sabathé F,Bélaïch A,Soucaille P

更新日期：2002-11-19 00:00:00

abstract：:The aims of this work were to characterize the 16S-23S internal spacer region of the fish pathogen Tenacibaculum soleae and to develop a PCR assay for its identification and detection. All T. soleae strains tested displayed a single internal spacer region class, containing tRNA(I) (le) and tRNA(A) ...

journal_title：FEMS microbiology letters

authors： López JR,Hamman-Khalifa AM,Navas JI,de la Herran R

更新日期：2011-11-01 00:00:00

abstract：:Corynebacterium diphtheriae, generally considered an extracellular coloniser, was evaluated for its ability to enter and survive within HEp-2 monolayers by gentamicin protection assay. Intracellular viability of HC01 strain, isolated from endocarditis, was more expressive (2.59%) than observed in 241 (0.21%) and CDC-E...

journal_title：FEMS microbiology letters

authors： Hirata R,Napoleão F,Monteiro-Leal LH,Andrade AF,Nagao PE,Formiga LC,Fonseca LS,Mattos-Guaraldi AL

更新日期：2002-09-24 00:00:00