Abstract:
:Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: However, the efficiency was too low and irreproducible to be useful in many genetic studies. Therefore, the effects on transformation efficiency of changing different components of competence-stimulating peptide-induced transformation have been examined. Screening of growth media was followed by optimization of pre-induction culture acidification, glycine concentration, and induction time. An optimized protocol was developed whereby S. pneumoniae strain JNR.7/87 was transformed reproducibly with a streptomycin resistance (SmR) marker at an efficiency of approximately 10(5) colony forming units per 10(8) cells.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Bricker AL,Camilli Adoi
10.1111/j.1574-6968.1999.tb13460.xkeywords:
subject
Has Abstractpub_date
1999-03-15 00:00:00pages
131-5issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00027-0journal_volume
172pub_type
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