Correlation between glycosylation of flagellin proteins and sensitivity of flagellar filaments to Triton X-100 in methanogens.

Abstract:

:The flagellins of Methanospirillum hungatei strains JF1 and GP1, Methanococcus deltae, and Methanothermus fervidus are glycosylated. Isolated flagellar filaments from these organisms are dissociated by low concentrations (0.5% (v/v)) of Triton X-100. Flagellar filaments from other methanogens (Methanococcus voltae, Methanococcus vannielii and Methanoculleus marisnigri) composed of non-glycosylated flagellins are resistant to Triton X-100 treatment. Consequently, the isolation techniques (employing Triton X-100) used to isolate basal body-hook-filament complexes in eubacteria may not be applicable to many methanogens.

journal_name

FEMS Microbiol Lett

authors

Faguy DM,Koval SF,Jarrell KF

doi

10.1016/0378-1097(92)90616-v

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

129-34

issue

2

eissn

0378-1097

issn

1574-6968

journal_volume

69

pub_type

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