Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae.

abstract：:We report here improvements to the growth media and fermentation conditions which result in a substantial increase of Helicobacter pylori growth and in the enhanced production of vacuolating cytotoxin. Addition of glucose to the medium resulted in the increase of cell yield, cell viability and a significant improvemen...

journal_title：FEMS microbiology letters

authors： Marchini A,Massari P,Manetti R,Olivieri R

更新日期：1994-11-15 00:00:00

abstract：:Tritrichomonas foetus is a venereal pathogen of cattle, which causes infertility, early embryonic death or abortion. In order to evaluate the potential trichomonicidal activity of benzimidazoles, the effects of thiabendazole, mebendazole and albendazole were analyzed on the multiplication, general morphology and ultra...

journal_title：FEMS microbiology letters

authors： Carvalho KP,Gadelha AP

更新日期：2007-10-01 00:00:00

abstract：:Saccharomyces cerevisiae strains tolerant to osmotic stress are important for ethanol production during very high gravity (VHG) fermentation. We aimed to identify novel genes that confer enhanced tolerance to osmotic stress in S. cerevisiae. Two strains tolerant to up to 30% (w/v) glucose were isolated by screening a ...

journal_title：FEMS microbiology letters

authors： Kim B,Kim HS

更新日期：2018-07-01 00:00:00

abstract：:Ruminococcus albus is a Gram-positive bacterium that degrades plant cell walls in the rumen of herbivores. It was described to synthesize two major glycoside-hydrolases (Cel9B and Cel48A), which are exported and anchored at the cell surface. In bacteria, proteins destined to cross the cytoplasmic membrane are synthesi...

journal_title：FEMS microbiology letters

authors： Esbelin J,Martin C,Forano E,Mosoni P

更新日期：2009-05-01 00:00:00

abstract：:The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

journal_title：FEMS microbiology letters

authors： Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

更新日期：1995-08-01 00:00:00

abstract：:It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

journal_title：FEMS microbiology letters

authors： Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

更新日期：2003-04-25 00:00:00

abstract：:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were i...

journal_title：FEMS microbiology letters

authors： Mehta A,Mehta YR,Rosato YB

更新日期：2002-05-21 00:00:00

abstract：:The benzimidazole fungicide thiophanate-methyl is commonly applied to control leaf mould of tomato caused by Cladosporium fulvum in China. In this study, 32 isolates of C. fulvum were examined for their sensitivities to thiophanate-methyl, and two benzimidazole-resistant (BenR) phenotypes BenR1 and BenR2 were identifi...

journal_title：FEMS microbiology letters

authors： Yan L,Chen J,Zhang C,Ma Z

更新日期：2008-01-01 00:00:00

abstract：:Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin ...

journal_title：FEMS microbiology letters

authors： Keren I,Kaldalu N,Spoering A,Wang Y,Lewis K

更新日期：2004-01-15 00:00:00

abstract：:Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured...

journal_title：FEMS microbiology letters

authors： Guan SM,Shu L,Fu SM,Liu B,Xu XL,Wu JZ

更新日期：2008-06-01 00:00:00

abstract：:The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

journal_title：FEMS microbiology letters

authors： Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

更新日期：1998-10-01 00:00:00

abstract：:Magnetotactic bacteria are microorganisms that respond to magnetic fields. We studied the surface ultrastructure of uncultured magnetotactic cocci collected from a marine environment by transmission electron microscopy using freeze-fracture and freeze-etching. All bacteria revealed a Gram-negative cell wall. Many bact...

journal_title：FEMS microbiology letters

authors： Freitas F,Keim CN,Kachar B,Farina M,Lins U

更新日期：2003-02-14 00:00:00

abstract：:Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriat...

journal_title：FEMS microbiology letters

authors： Cong Y,Wang J,Chen Z,Xiong K,Xu Q,Hu F

更新日期：2011-04-01 00:00:00

abstract：:Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

journal_title：FEMS microbiology letters

authors： Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

更新日期：1999-10-15 00:00:00

abstract：:Pseudomonas spp., in particular Pseudomonas fragi, are dominant in aerobically stored chilled meats. This work isolated P. fragi isolates from spoiled chicken and investigated the effect of modified atmosphere packaging (MAP, CO2/N2 30%/70%) on physicochemical properties of P. fragi isolates compared to the correspond...

journal_title：FEMS microbiology letters

authors： Wang GY,Li M,Ma F,Wang HH,Xu XL,Zhou GH

更新日期：2017-06-15 00:00:00

abstract：:We identified a novel type-III staphylococcal cassette chromosome mec (SCCmec) element carried by eight methicillin-resistant Staphylococcus aureus (MRSA) strains from different wards and patients in an Indian hospital. Although the pulsed-field gel electrophoresis pattern and spa types of eight strains were identical...

journal_title：FEMS microbiology letters

authors： Arakere G,Nadig S,Ito T,Ma XX,Hiramatsu K

更新日期：2009-03-01 00:00:00

abstract：:Methanotrophic populations have been studied in Newport Bay estuary, Southern California. Environmental clone banks were generated for 16S rRNA genes specific to methanotrophs and for a diagnostic functional gene, pmoA, encoding a conserved subunit of the particulate methane monooxygenase. These clone banks contained ...

journal_title：FEMS microbiology letters

authors： McDonald IR,Smith K,Lidstrom ME

更新日期：2005-09-15 00:00:00

abstract：:Triclosan is the active ingredient in a multitude of health care and consumer products with germicidal properties, which have flooded the market in recent years in response to the public's fear of communicable bacteria. Although originally thought to kill bacteria by attacking multiple cellular targets, triclosan was ...

journal_title：FEMS microbiology letters

authors： Schweizer HP

更新日期：2001-08-07 00:00:00

abstract：:The Mycobacterium tuberculosis murG gene, Rv2153, was expressed in Escherichia coli murG(Ts) strain OV58 on a plasmid under the control of the arabinose-inducible araBAD promoter. Mycobacterium tuberculosis murG rescued the growth of E. coli murG(Ts) at the nonpermissive temperature: transformants were only obtained i...

journal_title：FEMS microbiology letters

authors： Jha RK,Katagihallimath N,Hota SK,Das KS,de Sousa SM

更新日期：2012-01-01 00:00:00

abstract：:Yeast Cia2p is a component of the cytosolic Fe/S protein assembly (CIA) machinery. Initial studies of the CIA machinery were performed in yeast, but the precise role of Cia2p in this eukaryote is still unknown. We report that CIA2 deficiency results in impaired oxidative stress response, as evidenced by increased sens...

journal_title：FEMS microbiology letters

authors： Zhao W,Zheng HZ,Niu YJ,Yuan Y,Fang BX,Liu YN,Cai LH,Zhou ZJ,Liu XG

更新日期：2015-03-01 00:00:00

abstract：:The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impa...

journal_title：FEMS microbiology letters

authors： Liang YY,de Zamaroczy M,Arsène F,Paquelin A,Elmerich C

更新日期：1992-12-15 00:00:00

abstract：:The fungal inhibitory effects of strain Lactobacillus plantarum MiLAB 393, producing broad-spectrum antifungal compounds, were evaluated. A co-cultivation method was set up to monitor effects on fungal growth and protein expression of growing Aspergillus nidulans with L. plantarum MiLAB 393. The effects of inhibitory ...

journal_title：FEMS microbiology letters

authors： Ström K,Schnürer J,Melin P

更新日期：2005-05-01 00:00:00

abstract：:The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriop...

journal_title：FEMS microbiology letters

authors： Varga M,Kuntová L,Pantůček R,Mašlaňová I,Růžičková V,Doškař J

更新日期：2012-07-01 00:00:00

abstract：:High voltage electroporation has been investigated as a method for rapid recovery of plasmid and chromosomal DNA from the cyanobacteria Nostoc PCC 7121, Synechococcus PCC 7002, and Anabaena PCC 7120. Pulses of 18 kV/cm and higher applied to concentrated Nostoc cells carrying a shuttle plasmid (pRL25) resulted in copio...

journal_title：FEMS microbiology letters

authors： Moser D,Zarka D,Hedman C,Kallas T

更新日期：1995-05-15 00:00:00

abstract：:The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

journal_title：FEMS microbiology letters

authors： Teramoto H,Tanaka H,Wariishi H

更新日期：2004-05-15 00:00:00

abstract：:Certain Lactobacillus brevis strains are resistant to hop-derived compounds such as isohumulone and are able to grow in beer. In this study, we raised an antiserum against our beer spoilage laboratory strain L. brevis 578 which reacted with 23 of 24 beer spoilers and two of 13 non-spoilers in precipitation reactions u...

journal_title：FEMS microbiology letters

authors： Yasui T,Yoda K

更新日期：1997-06-15 00:00:00

abstract：:The cyanobacterium Synechocystis 6803 was tagged by chromosomal integration of the firefly luciferase gene, luc, resulting in the modified strain Synechocystis 6803-luc. The tagged cells were monitored in Baltic Sea microcosms both by detection of the luc gene by PCR amplification and by measurement of luc gene expres...

journal_title：FEMS microbiology letters

authors： Möller A,Norrby AM,Gustafsson K,Jansson J

更新日期：1995-06-01 00:00:00

abstract：:Microbial production of acetone and butanol was one of the first large-scale industrial fermentation processes of global importance. During the first part of the 20th century, it was indeed the second largest fermentation process, superseded in importance only by the ethanol fermentation. After a rapid decline after t...

journal_title：FEMS microbiology letters

authors： Sauer M

更新日期：2016-07-01 00:00:00

abstract：:Tachyzoites of Toxoplasma gondii multiply within the parasitophorous vacuole (PV) until the lysis of the host cell. This study was undertaken to evaluate the effect of hydroxyurea (a specific drug that arrests cell division at G1/S phase) on the multiplication of T. gondii tachyzoites in infected Vero cells. Infected ...

journal_title：FEMS microbiology letters

authors： de Melo EJ,Mayerhoffer RO,de Souza W

更新日期：2000-04-01 00:00:00

abstract：:Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to faci...

journal_title：FEMS microbiology letters

authors： Liao C,Seo SO,Lu T

更新日期：2016-05-01 00:00:00