CIA2 deficiency results in impaired oxidative stress response and enhanced intracellular basal UPR activity in Saccharomyces cerevisiae.

abstract：:The ultrastructure of hepatitis B virus surface antigen (HBsAg) particles produced by recombinant yeast cells was examined using high-resolution negative staining, and ice embedding, electron microscopy. With negative staining, the HBsAg particles were spherical to slightly ovoid with a mean diameter of 27.5 nm and co...

journal_title：FEMS microbiology letters

authors： Yamaguchi M,Sugahara K,Shiosaki K,Mizokami H,Takeo K

更新日期：1998-08-15 00:00:00

abstract：:A different mutation (SMR1B) to SMR1-410 (ilv2), which determines resistance to sulfometuron methyl in Saccharomyces cerevisiae, was cloned by PCR. Sequence analysis indicated a C to T change at position 575 of the ILV2 coding sequence, which results in a proline transition to leucine at position 192. Similarly to SMR...

journal_title：FEMS microbiology letters

authors： Xie Q,Jiménez A

更新日期：1996-04-01 00:00:00

abstract：:Matrix metalloproteinases (MMPs) play pivotal roles in inflammatory diseases including chronic periodontitis. The effects of Prevotella intermedia, a major periodontal pathogen, on MMP-9 production in primary human periodontal ligament (hPDL) cells were examined in the present study. MMP-9 mRNA expression was measured...

journal_title：FEMS microbiology letters

authors： Guan SM,Shu L,Fu SM,Liu B,Xu XL,Wu JZ

更新日期：2008-06-01 00:00:00

abstract：:We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, lambda Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by lambda Red and large deletions of both 117- and 165-kbp regions could be gener...

journal_title：FEMS microbiology letters

authors： Fukiya S,Mizoguchi H,Mori H

更新日期：2004-05-15 00:00:00

abstract：:Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifuga...

journal_title：FEMS microbiology letters

authors： Vágvölgyi C,Ferenczy L

更新日期：1991-08-15 00:00:00

abstract：:The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited ch...

journal_title：FEMS microbiology letters

authors： Mizoguchi K,Ohta H,Miyagi A,Kurihara H,Takashiba S,Kato K,Murayama Y,Fukui K

更新日期：1997-01-01 00:00:00

abstract：:An isolation procedure was developed to provide within one day microcystin-LR, a cyclic heptapeptide toxin from Microcystis aeruginosa PCC 7806. After ODS (octadecylsilyl) solid phase extraction, the crude toxin fraction was chromatographed using a strong anion exchange column. The toxin was eluted with 0.02 M ammoniu...

journal_title：FEMS microbiology letters

authors： Martin C,Sivonen K,Matern U,Dierstein R,Weckesser J

更新日期：1990-03-01 00:00:00

abstract：:A screening for hydrogen uptake (hup) genes in Rhizobium leguminosarum bv. viciae isolates from different locations within Spain identified no Hup+ strains, confirming the scarcity of the Hup trait in R. leguminosarum. However, five new Hup+ strains were isolated from Ni-rich soils from Italy and Germany. The hup gene...

journal_title：FEMS microbiology letters

authors： Fernández D,Toffanin A,Palacios JM,Ruiz-Argüeso T,Imperial J

更新日期：2005-12-01 00:00:00

abstract：:Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine fece...

journal_title：FEMS microbiology letters

authors： Vuddhakul V,Patararungrong N,Pungrasamee P,Jitsurong S,Morigaki T,Asai N,Nishibuchi M

更新日期：2000-01-15 00:00:00

abstract：:Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

journal_title：FEMS microbiology letters

authors： Liu WJ,Dong H,Peng XW,Wu QM

更新日期：2014-07-01 00:00:00

abstract：:The sequences of the epidermolytic toxins and V8 serine proteinase share about 25% identity, including the catalytic triad at the proteinase active centre. Here we have altered the putative ETA active-site serine-195 to glycine by site-directed mutagenesis. No epidermolytic activity was detected when up to 100-fold gr...

journal_title：FEMS microbiology letters

authors： Redpath MB,Foster TJ,Bailey CJ

更新日期：1991-06-15 00:00:00

abstract：:A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein encoded by the CUP1 gene as does a copper-resistant strain. The mechanism of expression of the gene is inducible by copper ions in the copper-resistant strain. However, assays of CUP1-specific mRNA revealed that the transcription...

journal_title：FEMS microbiology letters

authors： Tohoyama H,Inagawa A,Koike H,Inouhe M,Joho M,Murayama T

更新日期：1992-08-01 00:00:00

abstract：:The adhesion-promoting proteins (APP) (molecular mass approx. 300 kDa), which promote adhesion of Streptococcus mutans JBP (serotype c) to hydroxyapatite, were isolated from human submandibular-sublingual (SMSL) saliva by gel filtration on a Trisacryl GP2000 M column. The effects of hexoses, pentoses, methyl-pentoses,...

journal_title：FEMS microbiology letters

authors： Kishimoto E,Hay DI,Gibbons RJ

更新日期：1991-12-15 00:00:00

abstract：:The aecA and aecB loci map at 250 and 290 degrees, respectively, on the Bacillus subtilis chromosomal genetic map. The aecB locus has been proposed as the structural gene for aspartokinase II. From DNA sequence analyses and comparisons to the sequence of the aspartokinase II gene, it can be concluded that the structur...

journal_title：FEMS microbiology letters

authors： Petricek M,Rutberg L,Hederstedt L

更新日期：1989-10-01 00:00:00

abstract：:In order to investigate whether the iron acquisition mechanisms of Staphylococcus aureus are induced by iron restriction in vitro, we examined S. aureus ATCC 6538 for production of siderophore and expression of transferrin-binding protein (SA-tbp) in normal or deferrated brain heart infusion broth (BHI). Siderophore p...

journal_title：FEMS microbiology letters

authors： Lim Y,Shin SH,Lee SI,Kim IS,Rhee JH

更新日期：1998-06-01 00:00:00

abstract：:Specific non-typhoid Salmonella serovars carry large virulence plasmids that promote sustained extra-intestinal infections. These plasmids all share a highly conserved 8-kb region containing the spv operon, consisting of the regulatory spvR locus and the four structural spvABCD genes. The SpvR protein belongs to the L...

journal_title：FEMS microbiology letters

authors： Guiney DG,Fang FC,Krause M,Libby S

更新日期：1994-11-15 00:00:00

abstract：:Piscirickettsia salmonis is a novel, aggressive, facultative Gram-negative bacterium that drastically affects salmon production at different latitudes, with particular impact in southern Chile. Initially, P. salmonis was described as a Rickettsia-like, obligate, intracellular Alphaproteobacteria, but it was reclassifi...

journal_title：FEMS microbiology letters

authors： Marshall SH,Henríquez V,Gómez FA,Cárdenas C

更新日期：2011-01-01 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches for combating persistent bacterial infection. An alternative approach is regulation of bacterial virulence instead of growth suppression, which can readily lead to drug resistance. The virulence of the opportunistic human pathogen Pseud...

journal_title：FEMS microbiology letters

authors： Lee JH,Kim YG,Cho MH,Kim JA,Lee J

更新日期：2012-04-01 00:00:00

abstract：:The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404 +/- 40 kb. The chromosome sizes for the reference strains of the other serotypes range between...

journal_title：FEMS microbiology letters

authors： Chevallier B,Dugourd D,Tarasiuk K,Harel J,Gottschalk M,Kobisch M,Frey J

更新日期：1998-03-15 00:00:00

abstract：:We examined the effect of the pyridoxal 5'-phosphate (PLP) cofactor on the activity and stability of the psychrophilic alanine racemase, having a high catalytic activity at low temperature, from Bacillus psychrosaccharolyticus at high temperatures. The decrease in the enzyme activity at incubation temperatures over 40...

journal_title：FEMS microbiology letters

authors： Okubo Y,Yokoigawa K,Esaki N,Soda K,Misono H

更新日期：2000-11-15 00:00:00

abstract：:Rifampicin is a principal drug used to combat infections by mycobacteria and related organisms. Most strains of Mycobacterium are able to inactivate this antibiotic by ribosylation via an ADP-ribosylated intermediate. We found that this inactivation was inhibited by light at levels similar to those prevailing in labor...

journal_title：FEMS microbiology letters

authors： Dabbs ER,Quan S

更新日期：2000-01-01 00:00:00

abstract：:Brief exposure of Pseudomonas aeruginosa to a temperature of 10 degrees C or lower caused a significant leakage of the periplasmic beta-lactamase into the medium. The extent of leakage increased as the incubation temperature was lowered to 4 degrees C and reached a maximum at 0 degrees C. Cells grown in the presence o...

journal_title：FEMS microbiology letters

authors： Lei Y,Satake S,Ishii J,Nakae T

更新日期：1991-05-15 00:00:00

abstract：:Marine snow aggregates are microbial hotspots that support high bacterial abundance and activities. We conducted laboratory experiments to compare cell-specific bacterial protein production (BPP) and protease activity between free-living and attached bacteria. Natural bacterial assemblages attached to model aggregates...

journal_title：FEMS microbiology letters

authors： Grossart HP,Tang KW,Kiørboe T,Ploug H

更新日期：2007-01-01 00:00:00

abstract：:We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pair...

journal_title：FEMS microbiology letters

authors： Lightbody KL,Renshaw PS,Collins ML,Wright RL,Hunt DM,Gordon SV,Hewinson RG,Buxton RS,Williamson RA,Carr MD

更新日期：2004-09-01 00:00:00

abstract：:A new Volvariella volvacea gene encoding a carbohydrate esterase (CE) family 4 acetyl xylan esterase (AXE) (designated as VvaxeII) was cloned and characterized. The coded polypeptide had 253 amino acid residues, with the first 19 serving as a secretion signal peptide. The VvaxeII transcript levels were high when the f...

journal_title：FEMS microbiology letters

authors： Liu X,Ding S

更新日期：2009-06-01 00:00:00

abstract：:Shigella flexneri, which causes shigellosis in humans, evolved from Escherichia coli. The sequencing of Shigella genomes has revealed that a large number of insertion sequence (IS) elements (over 200 elements) reside in the genome. Although the presence of these elements has been noted previously and summarized, more ...

journal_title：FEMS microbiology letters

authors： Zaghloul L,Tang C,Chin HY,Bek EJ,Lan R,Tanaka MM

更新日期：2007-12-01 00:00:00

abstract：:Brucella abortus B19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. B19 differs from other B. abortus strains in its sensitivity to erythritol. We took advantage of a previously obtained erythritol sensitive Tn5 insertion mutant of B. abortu...

journal_title：FEMS microbiology letters

authors： Sangari FJ,García-Lobo JM,Agüero J

更新日期：1994-09-01 00:00:00

abstract：:We identified a novel type-III staphylococcal cassette chromosome mec (SCCmec) element carried by eight methicillin-resistant Staphylococcus aureus (MRSA) strains from different wards and patients in an Indian hospital. Although the pulsed-field gel electrophoresis pattern and spa types of eight strains were identical...

journal_title：FEMS microbiology letters

authors： Arakere G,Nadig S,Ito T,Ma XX,Hiramatsu K

更新日期：2009-03-01 00:00:00

abstract：:The detection of ancient microbial DNA offers a new approach for the study of infectious diseases, their occurrence, frequency and host-pathogen interaction in historic times and populations. Moreover, data obtained from skeletal and mummified tissue may represent an important completion of contemporary phylogenetic a...

journal_title：FEMS microbiology letters

authors： Zink AR,Reischl U,Wolf H,Nerlich AG

更新日期：2002-08-06 00:00:00

abstract：:Biochemical responses to cold and osmotic stresses overlap because each decreases the availability of free water. Since RNA-binding proteins are known to accumulate following cold stress and play key roles in regulating transcription termination, the effect of osmotic stress on expression of RNA-binding proteins was e...

journal_title：FEMS microbiology letters

authors： Mori S,Castoreno A,Mulligan ME,Lammers PJ

更新日期：2003-10-24 00:00:00