Fungal cytochrome P450s catalyzing hydroxylation of substituted toluenes to form their hydroxymethyl derivatives.


:The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitrobenzaldehyde and 4-nitrobenzoic acid, albeit slowly. Exogenously added 4-nitrobenzaldehyde and 4-nitrobenzoic acid were predominantly reduced to 4-NBA. The fungal formation of 4-NBA was inhibited by piperonyl butoxide, a cytochrome P450 inhibitor, suggesting the involvement of cytochrome P450 in the hydroxylation of the methyl group. Similarly, 2-, and 3-nitrotoluenes and 4-chlorotoluene were converted to the corresponding arylalcohols by P. chrysosporium. On the other hand, toluene and 4-methoxytoluene were not converted. Thus, P. chrysosporium possesses an alkyl hydroxylation activity against aromatic compounds substituted with a strong electron-withdrawing group.


FEMS Microbiol Lett


Teramoto H,Tanaka H,Wariishi H





Has Abstract


2004-05-15 00:00:00














  • Outer membrane proteins of Bacteroides fragilis grown in vivo.

    abstract::The outer membrane protein (OMP) profiles of four different strains of Bacteroides fragilis, as determined by Coomassie blue stained polyacrylamide gels, were compared after growth in broth culture and in the mouse peritoneal cavity. There was no induction of the expression of large quantities of novel OMP after growt...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Patrick S,Lutton DA

    更新日期:1990-09-01 00:00:00

  • Antifungal activity against plant pathogenic fungi of chaetoviridins isolated from Chaetomium globosum.

    abstract::The Chaetomium globosum strain F0142, which was isolated from barnyard grass, showed potent disease control efficacy against rice blast (Magnaporthe grisea) and wheat leaf rust (Puccinia recondita). Two antifungal substances were purified from broth from this organism and identified as chaetoviridins A and B. Chaetovi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park JH,Choi GJ,Jang KS,Lim HK,Kim HT,Cho KY,Kim JC

    更新日期:2005-11-15 00:00:00

  • Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii.

    abstract::Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

    更新日期:2011-06-01 00:00:00

  • Adherence characteristics of endocarditis-derived Streptococcus gallolyticus ssp. gallolyticus (Streptococcus bovis biotype I) isolates to host extracellular matrix proteins.

    abstract::Members of the Streptococcus bovis group are frequent colonizers of the intestinal tract, which can also cause endocarditis. However, their ability to adhere to and colonize host tissues and the factors associated with pathogenicity are largely unknown. Here, we assessed 17 endocarditis-derived human isolates [identif...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sillanpää J,Nallapareddy SR,Singh KV,Ferraro MJ,Murray BE

    更新日期:2008-12-01 00:00:00

  • Cloning, heterologous expression, and sequencing of the Proteus vulgaris glnAntrBC operon and implications of nitrogen control on heterologous urease expression.

    abstract::The glnAntrBC operon of Proteus vulgaris was cloned and heterologously expressed in Escherichia coli. The nucleotide sequence was determined. An open reading frame of 1407 bp was identified as the glnA gene and the deduced amino acid sequence showed 82% identity with the E. coli glutamine synthetase protein. Heterolog...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Steglitz-Mörsdorf U,Mörsdorf G,Kaltwasser H

    更新日期:1993-01-15 00:00:00

  • Cloning and characterization of the Halobacillus trueperi betH gene, encoding the transport system for the compatible solute glycine betaine.

    abstract::Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. A fragment of the glycine betaine transporter betH gene was obtained from the genome of H. trueperi with degenerate primers. Through Southern blot hybridization and inverse PCR, a 5.1 kb EcoRI fragment containing the complete betH ge...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lu W,Zhao B,Feng D,Yang S

    更新日期:2004-06-15 00:00:00

  • Biochemical characterization of alpha-amylase from the yeast Cryptococcus flavus.

    abstract::During our screening of amylolytic microorganisms from Brazilian fruits, we isolated a yeast strain classified as Cryptococcus flavus. When grown on starch-containing medium this strain exhibited the highest amylase production after 24 h of cultivation. The extracellular amylase from C. flavus was purified from the cu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wanderley KJ,Torres FA,Moraes LM,Ulhoa CJ

    更新日期:2004-02-16 00:00:00

  • Characterization of lyso-galactolipids, C-2 and C-3 O-acyl trigalactosylglycerol isomers, obtained from the lichenized fungus Dictyonema glabratum.

    abstract::A mixture of two lyso isomers of a galactolipid was obtained from Dictyonema glabratum. Aqueous hydrolysis gave rise to galactose and glycerol in a 3:1 molar ratio. ESI-MS spectroscopy gave, in the positive-ion mode, a pseudomolecular ion at m/z 839 and daughter ions with m/z 677, 600, 515 and 353, suggesting three ga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sassaki GL,Gorin PA,Iacomini M

    更新日期:2001-01-15 00:00:00

  • Roles of siderophore in manganese-oxide reduction by Shewanella oneidensis MR-1.

    abstract::Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kouzuma A,Hashimoto K,Watanabe K

    更新日期:2012-01-01 00:00:00

  • Mutagenic analysis of putative domain II and surface residues in mosquitocidal Bacillus thuringiensis Cry19Aa toxin.

    abstract::The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Roh JY,Nair MS,Liu XS,Dean DH

    更新日期:2009-06-01 00:00:00

  • Characterization of a Mycobacterium bovis BCG insertion sequence related to the IS21 family.

    abstract::The structure and distribution of a Mycobacterium bovis BCG insertion element of the IS21 family were investigated. Several IS21-like elements found in mycobacterial genomes were separated in four types, following their nucleic acid similarities. The M. bovis BCG IS21 element is highly similar to IS1533 (class I), 70%...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lefèvre P,Braibant M,Content J,Gilot P

    更新日期:1999-09-15 00:00:00

  • Molecular cloning and DNA sequence of dniR, a gene affecting anaerobic expression of the Escherichia coli hexaheme nitrite reductase.

    abstract::A gene responsible for increased synthesis of hexaheme nitrite reductase (cytochrome c552) of Escherichia coli K-12 was cloned into pBR322 by the direct immunological screening method using antiserum against the purified enzyme. The cloned gene was mapped at 5 min on the chromosomal linkage map as the dni gene (relate...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kajie S,Ideta R,Yamato I,Anraku Y

    更新日期:1991-10-01 00:00:00

  • Expression of the short fimbriae of Porphyromonas gingivalis is regulated in oral bacterial consortia.

    abstract::The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park Y,James CE,Yoshimura F,Lamont RJ

    更新日期:2006-09-01 00:00:00

  • Genetic investigation within Lactococcus garvieae revealed two genomic lineages.

    abstract::The diversity of a collection of 49 Lactococcus garvieae strains, including isolates of dairy, fish, meat, vegetable and cereal origin, was explored using a molecular polyphasic approach comprising PCR-ribotyping, REP and RAPD-PCR analyses and a multilocus restriction typing (MLRT) carried out on six partial genes (at...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Ferrario C,Ricci G,Borgo F,Rollando A,Fortina MG

    更新日期:2012-07-01 00:00:00

  • Pathogenesis of Vibrio vulnificus.

    abstract::This review describes the factors which are currently recognized as being central to the virulence of the human pathogen, Vibrio vulnificus. This estuarine/marine bacterium occurs in high numbers in molluscan shellfish, primarily oysters, and its ingestion in raw oysters results in a ca. 60% mortality in those persons...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Linkous DA,Oliver JD

    更新日期:1999-05-15 00:00:00

  • Overproduction of L-cysteine and L-cystine by expression of genes for feedback inhibition-insensitive serine acetyltransferase from Arabidopsis thaliana in Escherichia coli.

    abstract::Two cDNAs encoding feedback inhibition-insensitive serine acetyltransferases of Arabidopsis thaliana were expressed in the chromosomal serine acetyltransferase-deficient and L-cysteine non-utilizing Escherichia coli strain JM39-8. The transformants produced 1600 to 1700 mg l(-1) of L-cysteine and L-cystine from glucos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takagi H,Awano N,Kobayashi S,Noji M,Saito K,Nakamori S

    更新日期:1999-10-15 00:00:00

  • Distribution of insertion sequence IS1 in multiple-antibiotic resistant clinical Enterobacteriaceae strains.

    abstract::The presence of insertion sequence IS1 in 70 multiple-antibiotic resistant clinical strains was determined. This 70-strain collection comprised 46 Escherichia coli, 18 Salmonella and 6 Shigella strains. The presence of IS1 was detected in the chromosome and plasmids of 73% and 63% of the strains, respectively, and 51%...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ramirez-Santos J,Alvarez G,Cisneros E,Gomez-Eichelmann MC

    更新日期:1992-06-01 00:00:00

  • In vitro conjugal transfer of tetracycline resistance from Lactobacillus isolates to other Gram-positive bacteria.

    abstract::The ability of 14 Lactobacillus strains, isolated from fermented dry sausages, to transfer tetracycline resistance encoded by tet(M) through conjugation was examined using filter mating experiments. Seven out of 14 tetracycline-resistant Lactobacillus isolates were able to transfer in vitro this resistance to Enteroco...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gevers D,Huys G,Swings J

    更新日期:2003-08-08 00:00:00

  • Genetic analysis of Carboxydothermus hydrogenoformans carbon monoxide dehydrogenase genes cooF and cooS.

    abstract::Carboxydothermus hydrogenoformans is an extremely thermophilic, Gram-positive bacterium growing on carbon monoxide (CO) as single carbon and energy source and producing only H(2) and CO(2). Carbon monoxide dehydrogenase is a key enzyme for CO metabolism. The carbon monoxide dehydrogenase genes cooF and cooS from C. hy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: González JM,Robb FT

    更新日期:2000-10-15 00:00:00

  • Novel putative rhizobial species with different symbiovars nodulate Lotus creticus and their differential preference to distinctive soil properties.

    abstract::Phylogenetically diverse rhizobial strains endemic to Tunisia were isolated from symbiotic nodules of Lotus creticus, growing in different arid extremophile geographical regions of Tunisia, and speciated using multiloci-phylogenetic analysis as Neorhizobium huautlense (LCK33, LCK35, LCO42 and LCO49), Ensifer numidicus...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Rejili M,BenAbderrahim MA,Mars M,Sherrier JD

    更新日期:2020-06-01 00:00:00

  • Enzymic activity of salivary amylase when bound to the surface of oral streptococci.

    abstract::The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Douglas CW,Heath J,Gwynn JP

    更新日期:1992-04-15 00:00:00

  • Surface Listeria monocytogenes carbohydrate-binding components revealed by agglutination with neoglycoproteins.

    abstract::Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cottin J,Loiseau O,Robert R,Mahaza C,Carbonnelle B,Senet JM

    更新日期:1990-03-15 00:00:00

  • Cholesteryl-6-O-acyl-alpha-D-glucopyranoside of Helicobacter pylori relate to relative lysophospholipid content.

    abstract::The presence of cholesteryl glucosides and high levels of lysophospholipids are elements making the cell wall of Helicobacter pylori unique. In this study, we have investigated the relationship between lysophospholipid content and cholesteryl glucoside composition of variants of 6 clinical isolates. The samples were c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tannaes T,Bukholm G

    更新日期:2005-03-01 00:00:00

  • Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility.

    abstract::We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pair...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lightbody KL,Renshaw PS,Collins ML,Wright RL,Hunt DM,Gordon SV,Hewinson RG,Buxton RS,Williamson RA,Carr MD

    更新日期:2004-09-01 00:00:00

  • An endophytic/pathogenic Phoma sp. from creosote bush producing biologically active volatile compounds having fuel potential.

    abstract::A Phoma sp. was isolated and characterized as endophytic and as a pathogen of Larrea tridentata (creosote bush) growing in the desert region of southern Utah, USA. This fungus produces a unique mixture of volatile organic compounds (VOCs), including a series of sesquiterpenoids, some alcohols and several reduced napht...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Strobel G,Singh SK,Riyaz-Ul-Hassan S,Mitchell AM,Geary B,Sears J

    更新日期:2011-07-01 00:00:00

  • Comparison of the effect of salinity on the D/H ratio of fatty acids of heterotrophic and photoautotrophic microorganisms.

    abstract::The core metabolism of microorganisms has a major influence on the hydrogen isotopic composition of their fatty acids. Heterotrophic microorganisms produce fatty acids with a deuterium to hydrogen (D/H) ratio either slightly depleted or enriched in D compared to the growth water, while photo- and chemoautotrophic micr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Heinzelmann SM,Chivall D,M'Boule D,Sinke-Schoen D,Villanueva L,Damsté JS,Schouten S,van der Meer MT

    更新日期:2015-05-01 00:00:00

  • The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis.

    abstract::Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shima S,Yanagi M,Saiki H

    更新日期:1994-06-01 00:00:00

  • The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources.

    abstract::We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hoffmann N,Steinbüchel A,Rehm BH

    更新日期:2000-03-15 00:00:00

  • Conjugative transfer of a shuttle plasmid from Escherichia coli to Mycobacterium smegmatis [corrected].

    abstract::The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and successfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into Mycobacterium smeg...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lazraq R,Clavel-Sérès S,David HL,Roulland-Dussoix D

    更新日期:1990-05-01 00:00:00

  • Existence and characterization of allelic variants of Sao, a newly identified surface protein from Streptococcus suis.

    abstract::Surface antigen one (Sao) is a newly identified protein from the major zoonotic pathogen, Streptococcus suis. In search of functional proteins related to the pathogenesis of Chinese S. suis 2 (SS2), unexpectedly, a variant of Sao protein was obtained. To test its prevalence in S. suis, PCR assay was adopted to address...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Feng Y,Zheng F,Pan X,Sun W,Wang C,Dong Y,Ju AP,Ge J,Liu D,Liu C,Yan J,Tang J,Gao GF

    更新日期:2007-10-01 00:00:00