Abstract:
:The central carbon metabolism is well investigated in bacteria, but this is not the case for archaea. MJ0400-His(6) from Methanocaldococcus jannaschii catalyzes the cleavage of fructose-1,6-bisphosphate (FBP) to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate with a V(max) of 33 mU mg(-1) and a K(m) of 430 microM at 50 degrees C. MJ0400-His(6) is inhibited competitively by erythrose-4-phosphate with a K(i) of 380 microM and displays heat stability with a half-life of c. 1 h at 100 degrees C. Hence, MJ0400 is the second gene encoding for an FBP aldolase in M. jannaschii. Previously, MJ0400 was shown to act as an 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid synthase. This indicates that MJ0400 is involved in both the carbon metabolism and the shikimate pathway in M. jannaschii.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Samland AK,Wang M,Sprenger GAdoi
10.1111/j.1574-6968.2008.01079.xsubject
Has Abstractpub_date
2008-04-01 00:00:00pages
36-41issue
1eissn
0378-1097issn
1574-6968pii
FML1079journal_volume
281pub_type
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