Abstract:
:Six clinical isolates of Enterobacter cloacae isolated in a Portuguese hospital, between April 1999 and November 2000, demonstrated resistance to almost all broad-spectrum cephalosporins, except to cefepime. These isolates were susceptible to quinolones and to aminoglycosides. Isoelectric focusing demonstrated production of beta-lactamases with pIs > 8.0 and by all six isolates, exhibiting a cephalosporinase phenotype. The results of pulsed field gel electrophoresis revealed that these isolates were genetically unrelated. The amino acid sequence of six AmpC beta-lactamases (Eclo1FF, Eclo6FF, Eclo9FF, Eclo10FF, Eclo11FF and Eclo15FF) shared 97-99% homology with the chromosomal AmpC beta-lactamase from E. cloacae P99 and 86-87% homology with those of two plasmid-mediated AmpC beta-lactamases, MIR-1 and ACT-1. This is the first report of chromosomal AmpC beta-lactamase production by E. cloacae isolates in a Portuguese hospital.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Conceição T,Faria N,Lito L,Melo Cristino J,Salgado MJ,Duarte Adoi
10.1016/S0378-1097(03)00891-7keywords:
subject
Has Abstractpub_date
2004-01-30 00:00:00pages
197-202issue
2eissn
0378-1097issn
1574-6968pii
S0378109703008917journal_volume
230pub_type
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