Abstract:
:Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in non-phagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report the sequencing of the mycobacterial cell entry gene (mce) of Mycobacterium bovis bacillus Calmette-Guérin (BCG) and the generation and characterization of a mutant BCG strain with an inactivated mce gene, by homologous recombination with double cross-over. This mutant strain does not express the mycobacterial cell entry protein (Mce) and exhibits a reduced ability to invade the non-phagocytic epithelial cell line HeLa as compared to wild-type BCG.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Flesselles B,Anand NN,Remani J,Loosmore SM,Klein MHdoi
10.1111/j.1574-6968.1999.tb13738.xkeywords:
subject
Has Abstractpub_date
1999-08-15 00:00:00pages
237-42issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00301-8journal_volume
177pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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