Abstract:
:To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and an rsmA gene, were cloned and mutated by inserting with an aacC1 cassette, respectively. Two mutants showed the same regulatory mode with repression of phenazine-1-carboxylic acid and remarkable enhancement of pyoluteorin. In the rpoS-mutant, a translational rsmA'-'lacZ fusion was expressed at the same level corresponding to that in the wild-type strain. In the rsmA-mutant, however, expression of the translational rpoS'-'lacZ fusion was only about 30% of that in the wild-type strain. The results indicated that the absence of RsmA leads to repression of the rpoS gene expression, which has further been confirmed with construction of chromosomal rpoS-lacZ fusion in the rsmA-mutant and the wild-type strain, respectively. The findings showed a new regulatory cascade controlling antifungal metabolite production in Pseudomonas sp. M18, suggesting that RpoS may serve as a mediator in RsmA-dependent regulation of secondary metabolite biosynthesis.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Ge Y,Yang S,Fang Y,Yang R,Mou D,Cui J,Wen Ldoi
10.1111/j.1574-6968.2006.00562.xsubject
Has Abstractpub_date
2007-03-01 00:00:00pages
81-7issue
1eissn
0378-1097issn
1574-6968pii
FML562journal_volume
268pub_type
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