Abstract:
:Corynebacterium diphtheriae, generally considered an extracellular coloniser, was evaluated for its ability to enter and survive within HEp-2 monolayers by gentamicin protection assay. Intracellular viability of HC01 strain, isolated from endocarditis, was more expressive (2.59%) than observed in 241 (0.21%) and CDC-E8392 (1.93%) strains. Electron microscopy of C. diphtheriae-infected HEp-2 cells revealed intracellular bacteria inside membrane-bound vacuoles. Bacterial internalisation was totally inhibited by 5 microM cytochalasin E and significantly inhibited by 100 microM genistein (P<0.05). Therefore, C. diphtheriae presents the ability to survive within cultured epithelial cells and signalling cascade as well as actin polymerisation are required for entry of diphtheria bacilli into HEp-2 cells.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hirata R,Napoleão F,Monteiro-Leal LH,Andrade AF,Nagao PE,Formiga LC,Fonseca LS,Mattos-Guaraldi ALdoi
10.1111/j.1574-6968.2002.tb11379.xkeywords:
subject
Has Abstractpub_date
2002-09-24 00:00:00pages
115-9issue
1eissn
0378-1097issn
1574-6968pii
S0378109702009308journal_volume
215pub_type
杂志文章abstract::The Mycobacterium marinum G13 promoter is a sigma 70-like promoter that is more active by green fluorescent protein (gfp) differential fluorescence induction (DFI) assays when M. marium resides in an intracellular compartment as compared with growth in media. In assays using DFI, we found that the mycobacterial G13 pr...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2005.00078.x
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abstract::An inducible mycelial beta-glucosidase from Scytalidum thermophilum was characterized. The enzyme exhibited a pI of 6.5, a carbohydrate content of 15%, and an apparent molecular mass of about 40 kDa. Optima of temperature and pH were 60 degrees C and 6.5, respectively. The enzyme was stable up to 1 h at 50 degrees C a...
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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更新日期:2011-01-01 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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更新日期:2000-11-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:FEMS microbiology letters
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更新日期:2000-03-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:1997-12-15 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1093/femsle/fnx274
更新日期:2018-02-01 00:00:00
abstract::A purification scheme was devised for a 69-kDa outer membrane protein of Bordetella pertussis, a virulence-associated protein which may play a role in the pathogenesis of the organism. The protein was purified to apparent homogeneity by heating B. pertussis cells for 1 h at 60 degrees C followed by DEAE-Sepharose and ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(90)90010-n
更新日期:1990-02-01 00:00:00