当前位置： SCI文献检索 > FEMS MICROBIOLOGY LETTERS期刊下所有文献 > Differential green fluorescent protein expression from mycobacterial promoter constructs in Escherichia coli and Mycobacterium marinum.

Differential green fluorescent protein expression from mycobacterial promoter constructs in Escherichia coli and Mycobacterium marinum.

Abstract：

:The Mycobacterium marinum G13 promoter is a sigma 70-like promoter that is more active by green fluorescent protein (gfp) differential fluorescence induction (DFI) assays when M. marium resides in an intracellular compartment as compared with growth in media. In assays using DFI, we found that the mycobacterial G13 promoter was also more active in a background of lower nutrient availability during logarithmic growth. This promoter, contained in an insert cloned upstream of a gfp reporter gene, is also active in Escherichia coli. When gfp expression assays of different plasmid constructs were performed in parallel with E. coli and M. marinum, expression in E. coli was maintained after deletion of both upstream and/or downstream regions proximal to the core promoter sequence. In M. marinum, however, although upstream deletions had no appreciable effect on gfp expression, promoter constructs with deleted downstream regions expressed 20- to 40-fold less gfp over all growth phases. The high-level expression of gfp was restored, however, in a clone containing approximately 100 bp downstream of the transcriptional start point. We have therefore utilized this gfp reporter assay of promoter activity to distinguish possible differences in requirements for gfp expression between different genera that utilize sigma 70-like promoter elements. We found that high levels of expression of gfp from the G13 promoter in M. marinum require downstream regions not necessary for gfp expression in E. coli.

journal_name

FEMS Microbiol Lett

journal_title

FEMS microbiology letters

authors

Gall K,Barker LP

doi

10.1111/j.1574-6968.2005.00078.x

keywords:

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

301-7

issue

2

eissn

0378-1097

issn

1574-6968

pii

FML078

journal_volume

255

pub_type

杂志文章

相关文献

FEMS MICROBIOLOGY LETTERS文献大全
  • Secretory production of Arthrobacter levan fructotransferase from recombinant Escherichia coli.

    abstract：:Levan fructotransferase (LFTase) from Arthrobacter ureafaciens K2032 was expressed with N-terminal fusion of a LacZ-derived secretion motif (TMITNSSSVP) using the lac promoter system in recombinant Escherichia coli JM109 [pUDF-A81]. In flask cultures, recombinant enzyme activity was detected in culture media, and sequ...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2001.tb10509.x

    authors： Lee J,Saraswat V,Koh I,Song KB,Park YH,Rhee SK

    更新日期：2001-02-20 00:00:00

  • Mutation of rpoS enhances Pseudomonas sp. KL28 growth at higher concentrations of m-cresol and changes its surface-related phenotypes.

    abstract：:A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2006.00610.x

    authors： Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

    更新日期：2007-04-01 00:00:00

  • Accumulation of ppGpp in Streptococcus pyogenes and Streptococcus rattus following amino acid starvation.

    abstract：:We have re-examined the stringent response of Streptococcus rattus and Streptococcus pyogenes, two organisms that had originally been reported not to accumulate ppGpp following amino acid deprivation. We conclude that ppGpp does accumulate when S. rattus and S. pyogenes are deprived of isoleucine by mupirocin addition...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1998.tb12836.x

    authors： Whitehead KE,Webber GM,England RR

    更新日期：1998-02-01 00:00:00

  • Androgen binding proteins in Cochliobolus lunatus.

    abstract：:Testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus, a filamentous fungus. The maximal androgen binding occurred by 120 min at 0 degrees C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean Kd determ...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1016/0378-1097(93)90498-q

    authors： Kastelic-Suhadolc T,Lenasi H

    更新日期：1993-03-15 00:00:00

  • Regulation of nitrogen fixation in Azospirillum brasilense Sp7: involvement of nifA, glnA and glnB gene products.

    abstract：:The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impa...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1992.tb14028.x

    authors： Liang YY,de Zamaroczy M,Arsène F,Paquelin A,Elmerich C

    更新日期：1992-12-15 00:00:00

  • Sterols in a psychrophilic methanotroph, Methylosphaera hansonii.

    abstract：:A suite of six sterols, lanosterol, lanost-8(9)-en-3beta-ol, 4, 4-dimethylcholesta-8(14),24-dien-3beta-ol, 4, 4-dimethylcholest-8(14)-en-3beta-ol, 4-methylcholesta-8(14), 24-dien-3beta-ol and 4-methylcholest-8(14)-en-3beta-ol, were identified in the psychrophilic methanotrophic bacterium, Methylosphaera hansonii. Thei...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2000.tb09103.x

    authors： Schouten S,Bowman JP,Rijpstra WI,Sinninghe Damsté JS

    更新日期：2000-05-15 00:00:00

  • Purification and characterization of membrane-bound CO-reactive hemoprotein from Tetrahymena pyriformis mitochondria.

    abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1993.tb06423.x

    authors： Inokuchi A,Fukumori Y

    更新日期：1993-08-15 00:00:00

  • ERIC- and REP-PCR amplify non-repetitive fragments from the genome of Drechslera avenae and Stemphylium solani.

    abstract：:In the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were i...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2002.tb11202.x

    authors： Mehta A,Mehta YR,Rosato YB

    更新日期：2002-05-21 00:00:00

  • Bioinformatic and genetic characterization of three genes localized adjacent to the major replication origin of Haloferax volcanii.

    abstract：:In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1093/femsle/fnz238

    authors： Wolters M,Borst A,Pfeiffer F,Soppa J

    更新日期：2019-11-01 00:00:00

  • The Aspergillus niger acuA and acuB genes correspond to the facA and facB genes in Aspergillus nidulans.

    abstract：:Mutants in Aspergillus niger unable to grow on acetate as a sole carbon source were previously isolated by resistance to 1.2% propionate medium containing 0.1% glucose. AcuA mutants lacked acetyl-CoA synthetase (ACS) activity and acuB mutants lacked both ACS and isocitrate lyase activity. An acuA mutant was transforme...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1999.tb13756.x

    authors： Papadopoulou S,Sealy-Lewis HM

    更新日期：1999-09-01 00:00:00

  • CIA2 deficiency results in impaired oxidative stress response and enhanced intracellular basal UPR activity in Saccharomyces cerevisiae.

    abstract：:Yeast Cia2p is a component of the cytosolic Fe/S protein assembly (CIA) machinery. Initial studies of the CIA machinery were performed in yeast, but the precise role of Cia2p in this eukaryote is still unknown. We report that CIA2 deficiency results in impaired oxidative stress response, as evidenced by increased sens...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1093/femsle/fnv013

    authors： Zhao W,Zheng HZ,Niu YJ,Yuan Y,Fang BX,Liu YN,Cai LH,Zhou ZJ,Liu XG

    更新日期：2015-03-01 00:00:00

  • Validation of reference genes for normalization of qPCR mRNA expression levels in Staphylococcus aureus exposed to osmotic and lactic acid stress conditions encountered during food production and preservation.

    abstract：:Staphylococcus aureus represents the most prevalent cause of food-borne intoxications worldwide. While being repressed by competing bacteria in most matrices, this pathogen exhibits crucial competitive advantages during growth at high salt concentrations or low pH, conditions frequently encountered in food production ...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/1574-6968.12491

    authors： Sihto HM,Tasara T,Stephan R,Johler S

    更新日期：2014-07-01 00:00:00

  • The effect of some antibiotic-resistance-conferring plasmids on the removal of the heat-aggregated proteins from Escherichia coli cells.

    abstract：:We found that the presence of plasmids expressing tetracycline resistance or chloramphenicol resistance genes, but not those expressing ampicillin resistance or kanamycin resistance genes, in Escherichia coli led to the retardation of the process of removal of the heat-aggregated proteins (i.e. the S fraction) from th...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1999.tb13673.x

    authors： Kedzierska S,Staniszewska M,Potrykus J,Wegrzyn G

    更新日期：1999-07-15 00:00:00

  • Molecular characterization of a new acetyl xylan esterase (AXEII) from edible straw mushroom Volvariella volvacea with both de-O-acetylation and de-N-acetylation activity.

    abstract：:A new Volvariella volvacea gene encoding a carbohydrate esterase (CE) family 4 acetyl xylan esterase (AXE) (designated as VvaxeII) was cloned and characterized. The coded polypeptide had 253 amino acid residues, with the first 19 serving as a secretion signal peptide. The VvaxeII transcript levels were high when the f...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2009.01585.x

    authors： Liu X,Ding S

    更新日期：2009-06-01 00:00:00

  • 5-Carboxymethyl-2-hydroxymuconic semialdehyde dehydrogenases of Escherichia coli C and Klebsiella pneumoniae M5a1 show very high N-terminal sequence homology.

    abstract：:5-Carboxymethyl-2-hydroxymuconic semialdehyde (CHMS) dehydrogenase from Escherichia coli C and Klebsiella pneumoniae M5a1 have been purified and some of their properties studied. The apparent Km values for NAD and CHMS were 11.7 +/- 1.5 microM and 5.2 +/- 1.9 microM, respectively, for the K. pneumoniae enzyme, and 19....

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1016/0378-1097(89)90319-4

    authors： Fawcett T,Garrido-Pertierra A,Cooper RA

    更新日期：1989-02-01 00:00:00

  • Adherence to host extracellular matrix and serum components by Enterococcus faecium isolates of diverse origin.

    abstract：:Enterococcus faecium has emerged as an important cause of nosocomial infections over the last two decades. We recently demonstrated collagen type I (CI) as a common adherence target for some E. faecium isolates and a significant correlation was found to exist between acm-mediated CI adherence and clinical origin. Here...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2009.01806.x

    authors： Zhao M,Sillanpää J,Nallapareddy SR,Murray BE

    更新日期：2009-11-01 00:00:00

  • A molecular marker diagnostic of a specific isolate of an arbuscular mycorrhizal fungus, Gigaspora margarita.

    abstract：:To investigate the auto-ecology of a strain of Gigaspora margarita in a commercial inoculum, we found a pair of PCR primers amplifying a sequence of 235 bp diagnostic of the isolate. We designed an oligonucleotide probe based on the DNA sequence. The combination of PCR and the probing successfully detected the diagnos...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2002.tb11262.x

    authors： Yokoyama K,Tateishi T,Marumoto T,Saito M

    更新日期：2002-07-02 00:00:00

  • The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov.

    abstract：:The phylogenetic interrelationships of the genera Pediococcus and Aerococcus were investigated using reverse transcriptase sequencing of 16S rRNA. The genus Pediococcus was found to be phylogenetically heterogeneous. The four species P. acidilactici, P. damnosus, P. parvulus and P. pentosaceus formed a phylogeneticall...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1016/s0378-1097(05)80004-7

    authors： Collins MD,Williams AM,Wallbanks S

    更新日期：1990-08-01 00:00:00

  • The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source.

    abstract：:Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we tested whether this pathogen also uses a synthetic heme compound, Fe-alpha...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1999.tb13452.x

    authors： Miyoshi S,Kamei T,Inami Y,Ota Y,Yamamoto S,Tomochika K,Shinoda S

    更新日期：1999-03-01 00:00:00

  • A novel dextranase gene from the marine bacterium Bacillus aquimaris S5 and its expression and characteristics.

    abstract：:Dextranase specifically hydrolyzes dextran and is used to produce functional isomalto-saccharide prebiotics. Moreover, dextranase is used as an additive in mouthwash to remove dental plaque. We cloned and expressed the dextranase gene of the marine bacterium Bacillus aquimaris S5. The length of the BaDex gene was 1788...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1093/femsle/fnab007

    authors： Dong D,Wang X,Deng T,Ning Z,Tian X,Zu H,Ding Y,Wang C,Wang S,Lyu M

    更新日期：2021-01-20 00:00:00

  • Immuno-crossreactivity and toxicity assessment of conjugation products of the cyanobacterial toxin, microcystin-LR.

    abstract：:Immunoassays are increasingly used to investigate the production, properties and fates of the cyanobacterial hepatotoxic microcystins in vitro and in vivo. Responses of an ELISA immunoassay to microcystins have been determined using the authentic toxin antigen, microcystin-LR, and conjugation products between the toxi...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2000.tb09222.x

    authors： Metcalf JS,Beattie KA,Pflugmacher S,Codd GA

    更新日期：2000-08-15 00:00:00

  • Iron repressibility of siderophore and transferrin-binding protein in Staphylococcus aureus.

    abstract：:In order to investigate whether the iron acquisition mechanisms of Staphylococcus aureus are induced by iron restriction in vitro, we examined S. aureus ATCC 6538 for production of siderophore and expression of transferrin-binding protein (SA-tbp) in normal or deferrated brain heart infusion broth (BHI). Siderophore p...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1998.tb13020.x

    authors： Lim Y,Shin SH,Lee SI,Kim IS,Rhee JH

    更新日期：1998-06-01 00:00:00

  • Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci.

    abstract：:A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three add...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2002.tb11041.x

    authors： Oana K,Okimura Y,Kawakami Y,Hayashida N,Shimosaka M,Okazaki M,Hayashi T,Ohnishi M

    更新日期：2002-02-05 00:00:00

  • ADAMs are present in fungi: identification of two novel ADAM genes in Aspergillus fumigatus.

    abstract：:The ADAMs are a family of integral membrane proteases involved in shedding and fusion events in animal tissues. Here, we report the identification of two ADAMs, ADM-A and ADM-B, in the pathogenic fungus Aspergillus fumigatus. The domain structure of metazoan ADAMs was seen in ADM-A and -B, although with some differenc...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1016/j.femsle.2005.05.017

    authors： Lavens SE,Rovira-Graells N,Birch M,Tuckwell D

    更新日期：2005-07-01 00:00:00

  • Molecular characterization of benzimidazole-resistant isolates of Cladosporium fulvum.

    abstract：:The benzimidazole fungicide thiophanate-methyl is commonly applied to control leaf mould of tomato caused by Cladosporium fulvum in China. In this study, 32 isolates of C. fulvum were examined for their sensitivities to thiophanate-methyl, and two benzimidazole-resistant (BenR) phenotypes BenR1 and BenR2 were identifi...

    journal_title：FEMS microbiology letters

    pub_type： 信件

    doi：10.1111/j.1574-6968.2007.00999.x

    authors： Yan L,Chen J,Zhang C,Ma Z

    更新日期：2008-01-01 00:00:00

  • The intracellular proteolytic system of Yarrowia lipolytica and characterization of an aminopeptidase.

    abstract：:Intracellular proteases of Yarrowia lipolytica have been scarcely studied. These enzymes may play an important role in nitrogen metabolism, posttranslational processing, nutritional stress, dimorphism, etc.; biochemical and genetic control of these enzymes can help in obtaining high-level expression of recombinant pro...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2006.00578.x

    authors： Hernández-Montañez Z,Araujo-Osorio J,Noriega-Reyes Y,Chávez-Camarillo G,Villa-Tanaca L

    更新日期：2007-03-01 00:00:00

  • Physical and genetic map of the Listeria monocytogenes EGD serotype 1/2a chromosome.

    abstract：:Listeria monocytogenes is a facultative intracellular pathogen responsible for both invasive and non-invasive food-borne illness in animals and humans. In this study, macrorestriction analysis following pulsed-field gel electrophoresis was used to show that Listeria monocytogenes serovar 1/2a strain EGD has a single c...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.1999.tb13632.x

    authors： von Both U,Otten S,Darbouche A,Domann E,Chakraborty T

    更新日期：1999-06-15 00:00:00

  • Binding study of AfsK, a Ser/Thr kinase from Streptomyces coelicolor A3(2) and S-adenosyl-L-methionine.

    abstract：:Streptomyces coelicolor A3(2) produces an antibiotic, actinorhodin, which belongs to the aromatic polyketides and which can function as an acid/base indicator. Its production results in the death of microorganisms in the vicinity of S. coelicolor A3(2), and this phenomenon can be used in concert with biopesticides. Th...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2006.00531.x

    authors： Lee Y,Kim K,Suh JW,Rhee S,Lim Y

    更新日期：2007-01-01 00:00:00

  • Chitin degradation by cellulolytic anaerobes and facultative aerobes from soils and sediments.

    abstract：:Species of strictly and facultatively anaerobic cellulolytic bacteria from soils and sediments were examined for the ability to degrade chitin. Of 22 species studied, 16 degraded insoluble chitin. Cellulomonas uda, which was selected for a comparative study of its cellulase and chitinase enzyme systems, produced diffe...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1111/j.1574-6968.2001.tb10912.x

    authors： Reguera G,Leschine SB

    更新日期：2001-11-13 00:00:00

  • Quantification of Agrobacterium tumefaciens C58 attachment to Arabidopsis thaliana roots.

    abstract：:Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

    journal_title：FEMS microbiology letters

    pub_type： 杂志文章

    doi：10.1093/femsle/fnx158

    authors： Petrovicheva A,Joyner J,Muth TR

    更新日期：2017-10-02 00:00:00