Sterols in a psychrophilic methanotroph, Methylosphaera hansonii.

abstract：:The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

journal_title：FEMS microbiology letters

authors： Liao H,Jiang L,Zhang R

更新日期：2018-02-01 00:00:00

abstract：:The gene (open reading frame Tm1155, g6pd) encoding glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparent molecular mass of 95 kDa composed o...

journal_title：FEMS microbiology letters

authors： Hansen T,Schlichting B,Schönheit P

更新日期：2002-11-05 00:00:00

abstract：:OMIZ-W68, a chemically defined medium that contains no long-chain fatty acids and yet supports in vitro proliferation of a wide range of fastidious oral anaerobes, is described. The type strains of Treponema denticola, Treponema lecithinolyticum, Treponema maltophilum, Treponema pectinovorum, Treponema socranskii, and...

journal_title：FEMS microbiology letters

authors： Wyss C

更新日期：2007-04-01 00:00:00

abstract：:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed...

journal_title：FEMS microbiology letters

authors： Vierny C,Iaccarino M

更新日期：1989-07-01 00:00:00

abstract：:To tackle antimicrobial resistance (AMR) is of outmost importance for the general population to understand the severity and the relevance of different routes of transmission. Respondents of different age groups, educational and occupational backgrounds, area of living, diet and household composition participated in an...

journal_title：FEMS microbiology letters

authors： Alexa Oniciuc EA,Likotrafiti E,Garre A,Ruiz L,Prieto M,Alvarez-Ordóñez A

更新日期：2019-09-01 00:00:00

abstract：:The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 2...

journal_title：FEMS microbiology letters

authors： Moran AP,Shiberu B,Ferris JA,Knirel YA,Senchenkova SN,Perepelov AV,Jansson PE,Goldberg JB

更新日期：2004-12-01 00:00:00

abstract：:Four distinct bands of cysteine proteinase activity were detected when stationary-phase populations of Leishmania mexicana mexicana were subjected to gelatin-SDS-PAGE. The highest mobility band contained at least three isoforms separable by mono Q anion exchange chromatography. These high mobility activities were dist...

journal_title：FEMS microbiology letters

authors： Robertson CD,Coombs GH

更新日期：1992-07-01 00:00:00

abstract：:Chondroitin 6-sulfate depolymerizing activity was examined in the culture supernatant of Streptococcus intermedius ATCC 27335. 2-Acetamido-2-deoxy-3-O-(beta-D-gluco-4-enepyranosyluronic acid)-6-O-sulfo-D-galactose was split from the substrate. The enzyme(s) was not active upon chondroitin 4-sulfate or dermatan sulfate...

journal_title：FEMS microbiology letters

authors： Hibi E,Osano E,Fujii Y,Moriyama T

更新日期：1989-01-15 00:00:00

abstract：:Recent studies have generated interest in the use of the homologous recombination system of bacteriophage lambda for genetic engineering. The system, called Red, consists primarily of three proteins: lambda exonuclease, which processively digests the 5'-ended strand of a dsDNA end; beta protein, which binds to ssDNA a...

journal_title：FEMS microbiology letters

authors： Poteete AR

更新日期：2001-07-10 00:00:00

abstract：:Matrix proteins are the driving force of assembly of enveloped viruses. Their main function is to interact with and polymerize at cellular membranes and link other viral components to the matrix-membrane complex resulting in individual particle shapes and ensuring the integrity of the viral particle. Although matrix p...

journal_title：FEMS microbiology letters

authors： Timmins J,Ruigrok RW,Weissenhorn W

更新日期：2004-04-15 00:00:00

abstract：:Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type an...

journal_title：FEMS microbiology letters

authors： Juhas M,Wiehlmann L,Salunkhe P,Lauber J,Buer J,Tümmler B

更新日期：2005-01-15 00:00:00

abstract：:Bordetella calmodulin-like protein was purified from culture supernatant fluid of B. pertussis, B. parapertussis and B. bronchiseptica by successive chromatography on hydroxyapatite, Toyopearl HW-50F and QAE-Toyopearl 550C columns. The purified calmodulin-like protein appeared to be homogeneous by SDS-polyacrylamide g...

journal_title：FEMS microbiology letters

authors： Nagai M,Endoh M,Danbara H,Nakase Y

更新日期：1994-02-15 00:00:00

abstract：:Use of the green fluorescent protein (GFP) of Aequorea victoria as a reporter for protein and DNA localization has provided sensitive, new approaches for studying the organization of the bacterial cell, leading to new insights into diverse cellular processes. GFP has many characteristics that make it useful for locali...

journal_title：FEMS microbiology letters

authors： Phillips GJ

更新日期：2001-10-16 00:00:00

abstract：:Bananas contain large quantities of neurochemicals. Extracts from the peel and pulp of bananas in increasing stages of ripening were prepared and evaluated for their ability to modulate the growth of non-pathogenic and pathogenic bacteria. Extracts from the peel, and to a much lesser degree the pulp, increased the gro...

journal_title：FEMS microbiology letters

authors： Lyte M

更新日期：1997-09-15 00:00:00

abstract：:The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xyla...

journal_title：FEMS microbiology letters

authors： Gomez de Segura B,Durand R,Fèvre M

更新日期：1998-07-01 00:00:00

abstract：:Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral respo...

journal_title：FEMS microbiology letters

authors： Cunningham AF,Ward ME

更新日期：2003-10-10 00:00:00

abstract：:It is known that certain yeast strains, so called 'killers', can produce and excrete proteinaceous toxins that can induce death of other sensitive strains. We obtained a stable fungicidal factor (SKF) through concentration and stabilization of the excretion product of certain killer strains of Saccharomyces cerevisiae...

journal_title：FEMS microbiology letters

authors： Bracesco N,Salvo VA,Carrau FM,Nunes E

更新日期：2006-03-01 00:00:00

abstract：:The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K-, a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositio...

journal_title：FEMS microbiology letters

authors： Kogan G,Jann B,Jann K

更新日期：1992-03-01 00:00:00

abstract：:Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chlor...

journal_title：FEMS microbiology letters

authors： Kulakova AN,Reid KA,Larkin MJ,Allen CC,Kulakov LA

更新日期：1996-12-01 00:00:00

abstract：:The best yield of lipopolysaccharide (LPS) of Pseudomonas pseudomallei GIFU 12046 was obtained by extraction of defatted cells by phenol/chloroform/petroleum ether. The LPS showed a smooth character on SDS-polyacrylamide gel electrophoresis and contained D-glucose, L-glycero-D-manno-heptose, and D-glucosamine as the m...

journal_title：FEMS microbiology letters

authors： Kawahara K,Dejsirilert S,Danbara H,Ezaki T

更新日期：1992-09-15 00:00:00

abstract：:The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isot...

journal_title：FEMS microbiology letters

authors： Vázquez-Novelle MD,Pazos AJ,Abad M,Sánchez JL,Pérez-Parallé ML

更新日期：2005-02-01 00:00:00

abstract：:Control of Helminthosporium solani, the cause of silver scurf in potato tubers, has been impaired by selection of benzimidazole-resistant strains as a result of repeated use of the fungicide thiabendazole. Identification of thiabendazole-resistant strains of H. solani by conventional techniques takes several weeks. Pr...

journal_title：FEMS microbiology letters

authors： McKay GJ,Cooke LR

更新日期：1997-07-15 00:00:00

abstract：:Triclosan is the active ingredient in a multitude of health care and consumer products with germicidal properties, which have flooded the market in recent years in response to the public's fear of communicable bacteria. Although originally thought to kill bacteria by attacking multiple cellular targets, triclosan was ...

journal_title：FEMS microbiology letters

authors： Schweizer HP

更新日期：2001-08-07 00:00:00

abstract：:Streptococcus suis (S. suis) is an emerging zoonotic agent that exhibits high level resistance to classic antibiotics and a heavy burden in the swine industry. Therefore alternative antibacterial agents need to be developed. A novel endolysin derived from the S. suis temperate phage phi5218, termed Ply5218, was identi...

journal_title：FEMS microbiology letters

authors： Zhang H,Zhang C,Wang H,Yan YX,Sun J

更新日期：2016-09-01 00:00:00

abstract：:Using a specialized ribosome system, previous studies have identified G791 in Escherichia coli 16S rRNA as an invariant and essential residue for ribosome function. To investigate the functional role of G791, we searched for multicopy suppressors that partially restored the protein synthesis ability of mutant ribosome...

journal_title：FEMS microbiology letters

authors： Song WS,Ryou SM,Kim HM,Jeon CO,Kim JM,Han SH,Kim SW,Szatkiewicz JP,Cunningham PR,Lee K

更新日期：2010-12-01 00:00:00

abstract：:Specific non-typhoid Salmonella serovars carry large virulence plasmids that promote sustained extra-intestinal infections. These plasmids all share a highly conserved 8-kb region containing the spv operon, consisting of the regulatory spvR locus and the four structural spvABCD genes. The SpvR protein belongs to the L...

journal_title：FEMS microbiology letters

authors： Guiney DG,Fang FC,Krause M,Libby S

更新日期：1994-11-15 00:00:00

abstract：:Staphylococcus carnosus 833, inoculated into sausage meat, increased the level of methyl ketones, which contributed to the cured aroma. These ketones can arise from incomplete beta-oxidation followed by two enzymatic activities: a thioesterase and a decarboxylase. In this study we identified the beta-oxidative pathway...

journal_title：FEMS microbiology letters

authors： Engelvin G,Feron G,Perrin C,Mollé D,Talon R

更新日期：2000-09-01 00:00:00

abstract：:The quorum quenching (QQ) activity of endophytic bacteria associated with medicinal plants was explored. Extracts of the Gram-negative Enterobacter sp. CS66 possessed potent N-acylhomoserine lactone (AHL) hydrolytic activity in vitro. Using degenerate primers, we PCR-amplified an open reading frame (denoted aiiE) from...

journal_title：FEMS microbiology letters

authors： Shastry RP,Dolan SK,Abdelhamid Y,Vittal RR,Welch M

更新日期：2018-05-01 00:00:00

abstract：:This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

journal_title：FEMS microbiology letters

authors： Nastos S,Rangachari PK

更新日期：2016-08-01 00:00:00

abstract：:Neisseria gonorrhoeae and Neisseria meningitidis are exclusively human pathogens. A crucial property of the pathogenicity of neisserial infection is the ability to adhere to human epithelial cells. Pili mediate adherence of these bacteria to target cells and thereby promote colonization and infection of mucosal surfac...

journal_title：FEMS microbiology letters

authors： Jonsson AB

更新日期：1998-05-01 00:00:00