Isolation of Rhodococcus rhodochrous NCIMB13064 derivatives with new biodegradative abilities.

Abstract:

:Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chloroalkanes (C3-C8) is encoded by the same plasmid pRTL1. However, a different genetic element(s) is required for the dehalogenation of 3-chloropropionic acid. Two derivatives (P200 and P400) of R. rhodochrous NCIMB13064 were isolated which had acquired the ability to utilise naphthalene as sole carbon and energy source. Both strains lost the ability to utilise short-chain 1-chloroalkanes and underwent some rearrangements associated with pRTL1 plasmid.

journal_name

FEMS Microbiol Lett

authors

Kulakova AN,Reid KA,Larkin MJ,Allen CC,Kulakov LA

doi

10.1111/j.1574-6968.1996.tb08582.x

subject

Has Abstract

pub_date

1996-12-01 00:00:00

pages

227-31

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109796004144

journal_volume

145

pub_type

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