What makes the bacteriophage lambda Red system useful for genetic engineering: molecular mechanism and biological function.

abstract：:The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

journal_title：FEMS microbiology letters

authors： Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

更新日期：2002-06-18 00:00:00

abstract：:Rhodopirellula baltica (strain SH 1T) is a free-living marine representative of the phylogenetically independent and environmentally relevant phylum Planctomycetes. Little is known about the regulatory strategies of free-living bacteria with large (7.15 Mb) genomes. Therefore, a consistent, quantitative and qualitativ...

journal_title：FEMS microbiology letters

authors： Lombardot T,Bauer M,Teeling H,Amann R,Glöckner FO

更新日期：2005-01-01 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of...

journal_title：FEMS microbiology letters

authors： Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin S

更新日期：1995-12-01 00:00:00

abstract：:Effects of the two-component sensor-regulator pairs DctBD and NtrBC upon the expression of a dctA::phoA fusion from Rhizobium meliloti were determined under excess and limiting nitrogen concentrations in Escherichia coli. Results indicated that NtrBC affected transcription from the dctA promoter on a number of regulat...

journal_title：FEMS microbiology letters

authors： Allaway D,Boesten B,O'Gara F

更新日期：1995-05-15 00:00:00

abstract：:Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

journal_title：FEMS microbiology letters

authors： Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

更新日期：2005-02-01 00:00:00

abstract：:The ADP-ribosylating enzymes are encoded in many pathogenic bacteria in order to affect essential functions of the host. In this study, we show that Neisseria gonorrhoeae possess a locus that corresponds to the ADP-ribosyltransferase NarE, a previously characterized enzyme in N. meningitidis The 291 bp coding sequence...

journal_title：FEMS microbiology letters

authors： Rodas PI,Álamos-Musre AS,Álvarez FP,Escobar A,Tapia CV,Osorio E,Otero C,Calderón IL,Fuentes JA,Gil F,Paredes-Sabja D,Christodoulides M

更新日期：2016-09-01 00:00:00

abstract：:PM3 is an Aeromonas-specific bacteriophage which was isolated and characterized on A. hydrophila strain TF7. Spontaneous mutants resistant to PM3 were non-motile having lost their characteristic monopolar flagellum. In addition, purified flagella inactivated PM3. PM3 is the first filamentous bacteriophage isolated on ...

journal_title：FEMS microbiology letters

authors： Merino S,Camprubi S,Tomás JM

更新日期：1990-06-01 00:00:00

abstract：:The mol% G + C of DNA extracted from seven different isolates of Renibacterium salmoninarum was determined. Organisms studied were from selected geographical areas (U.S.A., Canada, England and France) and were isolated from five different species of salmonid fish. The mol% G + C was determined to be 55.5, higher than ...

journal_title：FEMS microbiology letters

authors： Banner CR,Rohovec JS,Fryer JL

更新日期：1991-03-15 00:00:00

abstract：:The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

journal_title：FEMS microbiology letters

authors： Alon RN,Gutnick DL

更新日期：1993-09-15 00:00:00

abstract：:Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

journal_title：FEMS microbiology letters

authors： Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

更新日期：1992-05-15 00:00:00

abstract：:Bacteria carry a number of genes that cause cell growth arrest or cell lysis upon expression. Notably, defective prophages retain many lysis proteins. Here, we identified a novel lytic gene, ydfD, on the Qin prophage segment of the Escherichia coli genome. YdfD lyses 99.9% of cells within 2 h of its induction. The co-...

journal_title：FEMS microbiology letters

authors： Masuda H,Awano N,Inouye M

更新日期：2016-03-01 00:00:00

abstract：:Eikenella corrodens 33EK(L), a clinical isolate, was assayed for its ability to utilise amino acids as substrates in the reduction of nitrate to nitrite. The metabolism of proline, glutamate, serine and glutamine was found to result in relatively high rates of nitrate reduction. The ability of cells to metabolise thes...

journal_title：FEMS microbiology letters

authors： Gully NJ,Rogers AH

更新日期：1996-06-01 00:00:00

abstract：:The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 x 10(-4)/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. kanamycin or neomycin...

journal_title：FEMS microbiology letters

authors： Sundström TK,Wieslander A

更新日期：1990-10-01 00:00:00

abstract：:To test the hypothesis that actA allelic variation contributes to virulence differences among Listeria monocytogenes strains, cell-to-cell spread and intracellular ActA phosphorylation patterns were characterized for 14 wild-type isolates and selected isogenic mutants. Our data show that (i) while actA allelic variati...

journal_title：FEMS microbiology letters

authors： Roberts AJ,Wiedmann M

更新日期：2006-01-01 00:00:00

abstract：:Phagemid vectors for display of protein/peptides on the surface of filamentous phage utilize a plasmid genome carrying the phage origin of replication, along with the gene fused to a fragment of gene III. Generation of phage particles displaying the fusion protein also requires superinfection of the host bacterium wit...

journal_title：FEMS microbiology letters

authors： Dueñas M,Borrebaeck CA

更新日期：1995-01-15 00:00:00

abstract：:The hepA gene in Anabaena sp. strain PCC 7120 is required for normal formation of the polysaccharide layer of the heterocyst envelope. A plasmid bearing hepA, interrupted by a neomycin-resistance cassette, was transferred by conjugation to wild type Anabaena variabilis ATCC 29413, so that the interrupted hepA gene rep...

journal_title：FEMS microbiology letters

authors： Leganés F

更新日期：1994-10-15 00:00:00

abstract：:This review is devoted to the mechanisms of antibiotic resistance in mollicutes (class Bacilli, subclass Mollicutes), the smallest self-replicating bacteria, that can cause diseases in plants, animals and humans, and also contaminate cell cultures and vaccine preparations. Research in this area has been mainly based o...

journal_title：FEMS microbiology letters

authors： Chernov VM,Chernova OA,Mouzykantov AA,Medvedeva ES,Baranova NB,Malygina TY,Aminov RI,Trushin MV

更新日期：2018-09-01 00:00:00

abstract：:Carboxydothermus hydrogenoformans is an extremely thermophilic, Gram-positive bacterium growing on carbon monoxide (CO) as single carbon and energy source and producing only H(2) and CO(2). Carbon monoxide dehydrogenase is a key enzyme for CO metabolism. The carbon monoxide dehydrogenase genes cooF and cooS from C. hy...

journal_title：FEMS microbiology letters

authors： González JM,Robb FT

更新日期：2000-10-15 00:00:00

abstract：:We have investigated the mechanisms used by Trichomonas vaginalis to damage cellular membranes, using human erythrocytes as target cells. Haemolysis is a contact- and temperature-dependent phenomenon, and is inhibited in 4 mM EGTA. Osmotic protection experiments using carbohydrates with different molecular diameters a...

journal_title：FEMS microbiology letters

authors： Fiori PL,Rappelli P,Rocchigiani AM,Cappuccinelli P

更新日期：1993-05-01 00:00:00

abstract：:The coiled-coil protein DivIVA is a determinant of apical growth and hyphal branching in Streptomyces coelicolor. We have investigated the properties of this protein and the involvement of different domains in its essential function and subcellular targeting. In S. coelicolor cell extracts, DivIVA was present as large...

journal_title：FEMS microbiology letters

authors： Wang SB,Cantlay S,Nordberg N,Letek M,Gil JA,Flärdh K

更新日期：2009-08-01 00:00:00

abstract：:Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-charact...

journal_title：FEMS microbiology letters

authors： Vos M,Sibleyras L,Lo LK,Hesse E,Gaze W,Klümper U

更新日期：2020-02-01 00:00:00

abstract：:A 3.5-kb native plasmid (pND103) was identified in Streptococcus thermophilus ST2-1. Preliminary sequence analysis indicated that pND103 belongs to group I S. thermophilus plasmids. A region of approximately 2 kb appears to contain three components: a plus origin of replication (ori) typical of plasmids that replicate...

journal_title：FEMS microbiology letters

authors： Su P,Jury K,Allison GE,Wong WY,Kim WS,Liu CQ,Vancov T,Dunn NW

更新日期：2002-10-29 00:00:00

abstract：:A bacterial strain, designated as TSB-6, was isolated from the sediments of a Tantloi (India) hot spring at 65 °C. The strain showed 98% 16S rRNA gene sequence similarity with Anoxybacillus kualawohkensis strain KW12 and was found to grow optimally at 37 °C. However, growing cells, cell suspensions, and cell-free extr...

journal_title：FEMS microbiology letters

authors： Paul SC,Jain P,Mitra J,Dutta S,Bhattacharya P,Bal B,Bhattacharyya D,Das Gupta S,Pal S

更新日期：2012-06-01 00:00:00

abstract：:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

journal_title：FEMS microbiology letters

authors： Edwards K,Linetsky I,Hueser C,Eisenstark A

更新日期：2001-05-30 00:00:00

abstract：:An accurate monitoring of disease progression is important to evaluate disease susceptibility phenotypes. Over the years, Arabidopsis thaliana has become the model species to serve as a host in plant-pathogen interactions. Despite the efforts to study genetic mechanisms of host defense, little efforts are made for a t...

journal_title：FEMS microbiology letters

authors： Brouwer M,Lievens B,Van Hemelrijck W,Van den Ackerveken G,Cammue BP,Thomma BP

更新日期：2003-11-21 00:00:00

abstract：:Using natural rubber latex as the sole source of carbon and energy 50 rubber-degrading bacteria were isolated. Out of those 50 isolates, 33 were identified as Streptomyces species and 8 as Micromonospora species. Screening of 1220 bacteria obtained from different culture collections revealed 46 additional rubber-degra...

journal_title：FEMS microbiology letters

authors： Jendrossek D,Tomasi G,Kroppenstedt RM

更新日期：1997-05-15 00:00:00

abstract：:Acetobacter spp. are used for industrial vinegar production because of their high ability to oxidize ethanol to acetic acid and high resistance to acetic acid. Two-dimensional gel electrophoretic analysis of a soluble fraction of Acetobacter aceti revealed the presence of several proteins whose production was enhanced...

journal_title：FEMS microbiology letters

authors： Nakano S,Fukaya M,Horinouchi S

更新日期：2004-06-15 00:00:00

abstract：:Shigella flexneri, which causes shigellosis in humans, evolved from Escherichia coli. The sequencing of Shigella genomes has revealed that a large number of insertion sequence (IS) elements (over 200 elements) reside in the genome. Although the presence of these elements has been noted previously and summarized, more ...

journal_title：FEMS microbiology letters

authors： Zaghloul L,Tang C,Chin HY,Bek EJ,Lan R,Tanaka MM

更新日期：2007-12-01 00:00:00

abstract：:A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three add...

journal_title：FEMS microbiology letters

authors： Oana K,Okimura Y,Kawakami Y,Hayashida N,Shimosaka M,Okazaki M,Hayashi T,Ohnishi M

更新日期：2002-02-05 00:00:00