Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis.

abstract：:While there is an extensive database of genes encoding ammonia monooxygenase (amo) found in the ammonia-oxidizing beta-proteobacteria, few amo sequences are available representing the gamma-proteobacteria. We sequenced the complete amo operon (amoCAB) for Nitrosococcus oceani (ATCC 19707), a marine, autotrophic, ammon...

journal_title：FEMS microbiology letters

authors： Alzerreca JJ,Norton JM,Klotz MG

更新日期：1999-11-01 00:00:00

abstract：:We have identified phosphatidylethanolamine as one of the major phospholipids of Clostridium perfringens by two dimensional thin layer chromatography of the intact lipids and of their deacylation products and by liquid chromatography followed by mass spectrometry of the intact neutral phospholipid fraction. The princi...

journal_title：FEMS microbiology letters

authors： Johnston NC,Baker JK,Goldfine H

更新日期：2004-04-01 00:00:00

abstract：:TraJ is an activator of the transfer (tra) operon in the F plasmid that counteracts H-NS silencing at the main transfer promoter (P(Y)). TraJ contains 226 aa (26 670 kDa), not 229 aa as reported previously, and forms homodimers. TraJ binds DNA containing P(Y)in vivo as demonstrated using a chromatin-immunoprecipitatio...

journal_title：FEMS microbiology letters

authors： Rodriguez-Maillard JM,Arutyunov D,Frost LS

更新日期：2010-09-01 00:00:00

abstract：:The structural gene, adhA, for a thermostable primary alcohol dehydrogenase was cloned from Thermoanaerobacter ethanolicus JW200. Constitutive expression from its own promoter was observed in Escherichia coli. The nucleotide sequence of adhA corresponded to an open reading frame of 1197 bp, encoding a polypeptide of 3...

journal_title：FEMS microbiology letters

authors： Holt PJ,Williams RE,Jordan KN,Lowe CR,Bruce NC

更新日期：2000-09-01 00:00:00

abstract：:A new rapid and simple method was developed for the detection of Escherichia coli by constructing a recombinant T4 phage carrying the cytochrome c peroxidase gene derived from Saccharomyces cerevisiae (T4ccp) using which, the colorimetric detection of E. coli K12 was examined. The oxidation activity toward the chromog...

journal_title：FEMS microbiology letters

authors： Hoang HA,Abe M,Nakasaki K

更新日期：2014-03-01 00:00:00

abstract：:Wood-rotting fungi possess remarkably diverse extracellular oxidation mechanisms, including enzymes, such as laccase and peroxidases, and Fenton chemistry. The ability to biologically drive Fenton chemistry by the redox cycling of quinones has previously been reported to be present in both ecologically diverging main ...

journal_title：FEMS microbiology letters

authors： Krueger MC,Bergmann M,Schlosser D

更新日期：2016-06-01 00:00:00

abstract：:This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

journal_title：FEMS microbiology letters

authors： Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

更新日期：2017-08-01 00:00:00

abstract：:Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. Howeve...

journal_title：FEMS microbiology letters

authors： Morton DJ,Madore LL,Smith A,Vanwagoner TM,Seale TW,Whitby PW,Stull TL

更新日期：2005-12-15 00:00:00

abstract：:We found the presence of DNA sequence which shows sequence similarity to the class IV chitin synthase gene (CHS3) of Saccharomyces cerevisiae in the genome of 14 Rhizopus species which belong to zygomycetes. We cloned a gene (chs3), which might correspond to one of these homologous sequences, from Rhizopus oligosporus...

journal_title：FEMS microbiology letters

authors： Motoyama T,Horiuchi H,Ohta A,Yamaguchi I,Takagi M

更新日期：1998-12-01 00:00:00

abstract：:PM3 is an Aeromonas-specific bacteriophage which was isolated and characterized on A. hydrophila strain TF7. Spontaneous mutants resistant to PM3 were non-motile having lost their characteristic monopolar flagellum. In addition, purified flagella inactivated PM3. PM3 is the first filamentous bacteriophage isolated on ...

journal_title：FEMS microbiology letters

authors： Merino S,Camprubi S,Tomás JM

更新日期：1990-06-01 00:00:00

abstract：:The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidat...

journal_title：FEMS microbiology letters

authors： Koyanagi T,Katayama T,Suzuki H,Kumagai H

更新日期：2004-08-01 00:00:00

abstract：:Streptococcus suis (S. suis) is an emerging zoonotic agent that exhibits high level resistance to classic antibiotics and a heavy burden in the swine industry. Therefore alternative antibacterial agents need to be developed. A novel endolysin derived from the S. suis temperate phage phi5218, termed Ply5218, was identi...

journal_title：FEMS microbiology letters

authors： Zhang H,Zhang C,Wang H,Yan YX,Sun J

更新日期：2016-09-01 00:00:00

abstract：:Haemophilus parasuis, the etiological agent of Glässer's disease in pigs, possesses iron acquisition pathways mediated by a surface receptor that specifically bind porcine transferrin. This receptor is composed of transferrin-binding protein A (TbpA) and TbpB. As it has been reported for other gram-negative organisms,...

journal_title：FEMS microbiology letters

authors： Martínez S,Frandoloso R,Rodríguez-Ferri EF,González-Zorn B,Gutiérrez-Martín CB

更新日期：2010-06-01 00:00:00

abstract：:The 16S rDNA of 17 strains of Azospirillum, 14 assigned to one of the known species A. amazonense, A. brasilense, A. halopraeferens, A. irakense and A. lipoferum, and the other three of uncertain taxonomic position, was sequenced after polymerase chain reaction amplification and analysed in order to investigate the ph...

journal_title：FEMS microbiology letters

authors： Fani R,Bandi C,Bazzicalupo M,Ceccherini MT,Fancelli S,Gallori E,Gerace L,Grifoni A,Miclaus N,Damiani G

更新日期：1995-06-15 00:00:00

abstract：:Phytochromes are red/far-red light photoreceptors found in plants, cyanobacteria and heterotrophic bacteria. Biochemical analyses have established that the genes bphO and bphP (PA4116 and PA4117) of Pseudomonas aeruginosa encode both phytochrome components: BphO, a heme oxygenase that produces the linear tetrapyrrole ...

journal_title：FEMS microbiology letters

authors： Barkovits K,Harms A,Benkartek C,Smart JL,Frankenberg-Dinkel N

更新日期：2008-03-01 00:00:00

abstract：:Biochemical responses to cold and osmotic stresses overlap because each decreases the availability of free water. Since RNA-binding proteins are known to accumulate following cold stress and play key roles in regulating transcription termination, the effect of osmotic stress on expression of RNA-binding proteins was e...

journal_title：FEMS microbiology letters

authors： Mori S,Castoreno A,Mulligan ME,Lammers PJ

更新日期：2003-10-24 00:00:00

abstract：:A typing method for Clostridium difficile based on restriction fragment length polymorphisms (RFLP) is described. The technique utilizes commercially available Escherichia coli ribosomal ribonucleic acid (rRNA) as probe material. Probe labelling, hybridization and detection was performed using the Enhanced Chemilumine...

journal_title：FEMS microbiology letters

authors： Bowman RA,O'Neill GL,Riley TV

更新日期：1991-04-15 00:00:00

abstract：:Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to p...

journal_title：FEMS microbiology letters

authors： Powell NG,Threlfall EJ,Chart H,Rowe B

更新日期：1994-06-01 00:00:00

abstract：:Mini-Tn10luxABcam/Ptac-ATS was constructed in order to develop a luciferase-transducing bacteriophage for detecting Escherichia coli O157:H7. The transposon was designed to deliver a 3.6-kb insertion that confers n-decanal-dependent bioluminescence and resistance to chloramphenicol and was constructed using mini-Tn10c...

journal_title：FEMS microbiology letters

authors： Waddell TE,Poppe C

更新日期：2000-01-15 00:00:00

abstract：:A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

journal_title：FEMS microbiology letters

authors： Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

更新日期：2002-10-29 00:00:00

abstract：:Clostridium difficile, a Gram-positive spore-forming anaerobe, causes infections in humans ranging from mild diarrhoeal to potentially life-threatening pseudomembranous colitis. The availability of genomic information for a range of C. difficile strains affords researchers the opportunity to better understand not only...

journal_title：FEMS microbiology letters

authors： Jain S,Graham RL,McMullan G,Ternan NG

更新日期：2010-11-01 00:00:00

abstract：:The branched-chain amino acid permease Bap2p is a transport system for leucine, isoleucine, and valine in Saccharomyces cerevisiae, and its synthesis is regulated transcriptionally. However, the downregulation mechanisms of Bap2p have not been established. Here we demonstrate that the C-terminal region of Bap2p plays ...

journal_title：FEMS microbiology letters

authors： Omura F,Kodama Y,Ashikari T

更新日期：2001-01-15 00:00:00

abstract：:Type II toxin-antitoxin systems (TAs) are small autoregulated bicistronic operons that encode a toxin protein with the potential to inhibit metabolic processes and an antitoxin protein to neutralize the toxin. Most of the bacterial genomes encode multiple TAs. However, the diversity and accumulation of TAs on bacteria...

journal_title：FEMS microbiology letters

authors： Ramisetty BC,Santhosh RS

更新日期：2016-02-01 00:00:00

abstract：:We have constructed a DNA library from a virulent Salmonella typhimurium strain, in an avirulent strain. The process of selecting the components of interest from the library involved iterative growth in liquid culture. This resulted, after four cycles, in the culture becoming homogeneous for a single plasmid, which wa...

journal_title：FEMS microbiology letters

authors： Lodge JM,Robey M,Stephen J,Brown NL

更新日期：1999-01-15 00:00:00

abstract：:Rainbow trout gastroenteritis has been related to the accumulation of segmented filamentous bacteria in the digestive tract of fish, which presents lethargy, reduced appetite and accumulation of mucoid faeces. Some authors associate the comparison of illness with the presence of viable filaments, which produce and rel...

journal_title：FEMS microbiology letters

authors： Manzano M,Giusto C,Iacumin L,Patthey C,Cecchini F,Fontanillas R,Comi G

更新日期：2010-07-01 00:00:00

abstract：:Legionella pneumophila, an intracellular parasite of macrophages and protozoa, requires iron for extra- and intracellular growth. In a new screen of a mutant library of L. pneumophila for strains defective for growth on agar media lacking supplemental iron, seven mutants were obtained. All of the mutants had a disrupt...

journal_title：FEMS microbiology letters

authors： Naylor J,Cianciotto NP

更新日期：2004-12-15 00:00:00

abstract：:Two thermostable phytases were identified from Thai isolates of Aspergillus japonicus BCC18313 (TR86) and Aspergillus niger BCC18081 (TR170). Both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into Pichia pastoris. The recombinant phytases, r-PhyA86 and r...

journal_title：FEMS microbiology letters

authors： Promdonkoy P,Tang K,Sornlake W,Harnpicharnchai P,Kobayashi RS,Ruanglek V,Upathanpreecha T,Vesaratchavest M,Eurwilaichitr L,Tanapongpipat S

更新日期：2009-01-01 00:00:00

abstract：:Eighty-six strains of S. thermophilus were examined for their plasmid content. Thirteen strains were found to contain one or two plasmids ranging in size from 2.1 to 7.4 kb. DNA-DNA hybridization analysis revealed the presence of five distinct groups of DNA homology. The complete nucleotide sequence of plasmid pST1 (A...

journal_title：FEMS microbiology letters

authors： Janzen T,Kleinschmidt J,Neve H,Geis A

更新日期：1992-08-15 00:00:00

abstract：:Bananas contain large quantities of neurochemicals. Extracts from the peel and pulp of bananas in increasing stages of ripening were prepared and evaluated for their ability to modulate the growth of non-pathogenic and pathogenic bacteria. Extracts from the peel, and to a much lesser degree the pulp, increased the gro...

journal_title：FEMS microbiology letters

authors： Lyte M

更新日期：1997-09-15 00:00:00

abstract：:Streptococcus equi ssp. equi is the causative agent of strangles, a highly contagious and serious disease in the upper respiratory tract of horses. The present study describes the characterization of IdeE, a homolog of the secreted IgG-specific protease IdeS/Mac of Streptococcus pyogenes. The activity of IdeE is compa...

journal_title：FEMS microbiology letters

authors： Lannergård J,Guss B

更新日期：2006-09-01 00:00:00