GeneChip expression analysis of the VqsR regulon of Pseudomonas aeruginosa TB.

Abstract:

:Two interlinked quorum sensing circuits, las and rhl, which control pathogenesis of Pseudomonas aeruginosa are further modulated by numerous regulators, including VqsR (virulence and quorum sensing regulator). High-density oligonucleotide microarrays were used to compare the global expression profile of a wild-type and VqsR mutant in ABC minimal medium. The expression of a large group of metabolic genes, ECF sigma factors as well as of many quorum-sensing genes previously not assigned to VqsR-regulon was found to be affected by the disruption of vqsR.

journal_name

FEMS Microbiol Lett

authors

Juhas M,Wiehlmann L,Salunkhe P,Lauber J,Buer J,Tümmler B

doi

10.1016/j.femsle.2004.11.020

keywords:

subject

Has Abstract

pub_date

2005-01-15 00:00:00

pages

287-95

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(04)00829-8

journal_volume

242

pub_type

杂志文章
  • Investigation of direct vs. indirect involvement of the c-type cytochrome MacA in Fe(III) reduction by Geobacter sulfurreducens.

    abstract::The electron transfer pathway to Fe(III) reduction in Geobacter sulfurreducens has been hypothesized to consist of a series of c-type cytochromes. Previous genetic studies suggested that the inner membrane-associated, c-type cytochrome, MacA, was a component of the electron transfer chain leading to Fe(III) reduction ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01252.x

    authors: Kim BC,Lovley DR

    更新日期:2008-09-01 00:00:00

  • Changes in the virulence of Mycobacterium avium after passage through embryonated hens' eggs.

    abstract::Eight-day-old embryonated hen's eggs were used as a model to study Mycobacterium avium virulence. Strains isolated from human patients caused 20-90% mortality when eggs were infected by injection of bacterial suspensions into the amniotic sac. Virulence of examined strains subsequently decreased with passage through e...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09297.x

    authors: Long EG,Ewing EP Jr,Bartlett JH,Horsburgh CR Jr,Birkness KA,Yakrus MA,Newman GW,Quinn FD

    更新日期:2000-09-15 00:00:00

  • Involvement of the mpo operon in resistance to class IIa bacteriocins in Listeria monocytogenes.

    abstract::High resistance to class IIa bacteriocins in Listeria monocytogenes has been clearly linked to lack of expression of the mptACD operon, encoding the EIIt Man mannose PTS permease. Also, intermediate resistance has been associated with membrane phospholipid modifications in the spontaneous mutants L. monocytogenes B73-...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.014

    authors: Arous S,Dalet K,Héchard Y

    更新日期:2004-09-01 00:00:00

  • Purification and characteristics of cytosolic chitinase from Piromyces communis OTS1.

    abstract::A chitinase was purified from the cytosolic fraction of the anaerobic rumen fungus Piromyces communis OTS1 by affinity chromatography using regenerated chitin, gel filtration and chromatofocusing. The chitinase was most active at pH 6.2 and at 60 degrees C in a 20-min assay. The molecular mass of the purified protein ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08085.x

    authors: Sakurada M,Morgavi DP,Komatani K,Tomita Y,Onodera R

    更新日期:1996-03-15 00:00:00

  • Accumulation of PHA granules in Cupriavidus necator as seen by confocal fluorescence microscopy.

    abstract::Many bacteria are capable of accumulating intracellular granules of polyhydroxyalkanoates (PHA). In this work, we developed confocal microscopy analysis of bacterial cells to study changes in the diameters of cells as well as PHA granules during growth and PHA accumulation in the bacterium Cupriavidus necator H16 (for...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw094

    authors: Mravec F,Obruca S,Krzyzanek V,Sedlacek P,Hrubanova K,Samek O,Kucera D,Benesova P,Nebesarova J

    更新日期:2016-05-01 00:00:00

  • Persister cells and tolerance to antimicrobials.

    abstract::Bacterial populations produce persister cells that neither grow nor die in the presence of microbicidal antibiotics. Persisters are largely responsible for high levels of biofilm tolerance to antimicrobials, but virtually nothing was known about their biology. Tolerance of Escherichia coli to ampicillin and ofloxacin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00856-5

    authors: Keren I,Kaldalu N,Spoering A,Wang Y,Lewis K

    更新日期:2004-01-15 00:00:00

  • Glucomannan utilization operon of Bacillus subtilis.

    abstract::This study characterizes the glucomannan utilization operon (gmuBACDREFG, formerly ydhMNOPQRST) of Bacillus subtilis. Transcription of the operon is induced by konjac glucomannan and requires the last mannanase gene (gmuG). Cellobiose and mannobiose, possible degradation products of glucomannan by GmuG, are strong ind...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.01018.x

    authors: Sadaie Y,Nakadate H,Fukui R,Yee LM,Asai K

    更新日期:2008-02-01 00:00:00

  • Exploitation of a chromosomally integrated lactose operon for controlled gene expression in Lactococcus lactis.

    abstract::Lactococcus lactis MG5267 is a plasmid-free strain in which the lactose operon is integrated in the bacterial chromosome. The chromosomal lacG gene which encodes phospho-beta-galactosidase was inactivated by a double cross-over integration event. Unexpectedly, the resultant mutant was shown to retain a Lac-positive ph...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(95)00474-2

    authors: Payne J,MacCormick CA,Griffin HG,Gasson MJ

    更新日期:1996-02-01 00:00:00

  • Glucose-6-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: expression of the g6pd gene and characterization of an extremely thermophilic enzyme.

    abstract::The gene (open reading frame Tm1155, g6pd) encoding glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49) of the hyperthermophilic bacterium Thermotoga maritima was cloned and functionally expressed in Escherichia coli. The purified recombinant enzyme is a homodimer with an apparent molecular mass of 95 kDa composed o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11443.x

    authors: Hansen T,Schlichting B,Schönheit P

    更新日期:2002-11-05 00:00:00

  • The ligase chain reaction distinguishes hepatitis B virus S-gene variants.

    abstract::A novel method for the identification of hepatitis B virus (HBV) variants was developed. The ligase chain reaction (LCR) distinguished the sequences of two isolates from the Gambia showing a change at nucleotide 421 within the region coding for the surface protein a antigenic determinant. One sequence was derived from...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07766.x

    authors: Karthigesu VD,Mendy M,Fortuin M,Whittle HC,Howard CR,Allison LM

    更新日期:1995-09-01 00:00:00

  • Polyhydroxyalkanoate biosynthesis in Pseudomonas pseudoalcaligenes YS1.

    abstract::Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (FADB::...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11247.x

    authors: Hang X,Lin Z,Chen J,Wang G,Hong K,Chen GQ

    更新日期:2002-06-18 00:00:00

  • Construction and characterization of a Xanthomonas oryzae pv. oryzae bacterial artificial chromosome library.

    abstract::Xanthomonas oryzae pv. oryzae is an important plant pathogen which causes bacterial blight of rice. To facilitate genome studies of this bacterium, we have constructed a bacterial artificial chromosome (BAC) library of strain MAFF 311018. It consisted of 750 clones representing 16 genome equivalents, and had an insert...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10693.x

    authors: Ochiai H,Inoue Y,Hasebe A,Kaku H

    更新日期:2001-06-12 00:00:00

  • The effects of oligosaccharide trimming inhibitors on glycoprotein expression and infectivity of Junin virus.

    abstract::The effects of specific inhibitors of glycoprotein trimming reactions on Junin virus (JV) replication were investigated. Bromoconduritol, an inhibitor of glucosidase II, significantly reduced infective virus production (DE50: 1.1 mM) and viral protein expression. Neither 1-deoxynojirimycin, an inhibitor of both glucos...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06140.x

    authors: Silber AM,Candurra NA,Damonte EB

    更新日期:1993-05-01 00:00:00

  • Not all animals need a microbiome.

    abstract::It is often taken for granted that all animals host and depend upon a microbiome, yet this has only been shown for a small proportion of species. We propose that animals span a continuum of reliance on microbial symbionts. At one end are the famously symbiont-dependent species such as aphids, humans, corals and cows, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1093/femsle/fnz117

    authors: Hammer TJ,Sanders JG,Fierer N

    更新日期:2019-05-01 00:00:00

  • Biotype and macromolecular profiles of cytotoxin-producing strains of Helicobacter pylori from antral gastric mucosa.

    abstract::Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90086-p

    authors: Owen RJ,Bickley J,Moreno M,Costas M,Morgan DR

    更新日期:1991-04-15 00:00:00

  • Mycobacterium marinum biofilm formation reveals cording morphology.

    abstract::Abstract The emergence of the nontuberculosis mycobacteria (NTM) as clinically relevant pathogens has warranted the study of these ubiquitous organisms in the context of their likely environmental niche, the biofilm. We assayed the NTM bacterium Mycobacterium marinum strain 1218R, a fish outbreak isolate, for biofilm ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00143.x

    authors: Hall-Stoodley L,Brun OS,Polshyna G,Barker LP

    更新日期:2006-04-01 00:00:00

  • RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion.

    abstract::It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00838.x

    authors: Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

    更新日期:2007-09-01 00:00:00

  • The phosphotransferase system gene ptsI in the endophytic bacterium Bacillus cereus is required for biofilm formation, colonization, and biocontrol against wheat sharp eyespot.

    abstract::Natural resistance of wheat plants to wheat sharp eyespot is inadequate, and new strategies for controlling the disease are required. Biological control is an alternative and attractive way of reducing the use of chemicals in agriculture. In this study, we investigated the biocontrol properties of endophytic bacterium...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12438

    authors: Xu YB,Chen M,Zhang Y,Wang M,Wang Y,Huang QB,Wang X,Wang G

    更新日期:2014-05-01 00:00:00

  • Far different levels of gene expression provided by an oriented cloning system in Bacillus subtilis and Escherichia coli.

    abstract::A gene expression system for both Bacillus subtilis and Escherichia coli was developed. The expression vector, pHASH102, produces any combination of promoter and open reading frame to be expressed based on the T-extended cloning method. Because the pHASH series vectors are designed to shuttle between the genome and a ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00171-X

    authors: Ohashi Y,Ohshima H,Tsuge K,Itaya M

    更新日期:2003-04-11 00:00:00

  • Phase I and phase II enzymes produced by Cunninghamella elegans for the metabolism of xenobiotics.

    abstract::The filamentous fungus Cunninghamella elegans has the ability to metabolize xenobiotics, including polycyclic aromatic hydrocarbons and pharmaceutical drugs, by both phase I and II biotransformations. Cytosolic and microsomal fractions were assayed for activities of cytochrome P450 monooxygenase, aryl sulfotransferase...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08161.x

    authors: Zhang D,Yang Y,Leakey JE,Cerniglia CE

    更新日期:1996-05-01 00:00:00

  • Functional interactions between homologous conditional killer systems of plasmid and chromosomal origin.

    abstract::parD and chpA are homologous conditional killer systems of plasmid and chromosomal origin, respectively, encoding a killer protein (Kid and ChpAK) and an antidote (Kis and ChpAI). Here it is shown that these systems can functionally interact. A multicopy chpA recombinant partially complements two mutations in the anti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10408.x

    authors: Santos-Sierra S,Giraldo R,Díaz-Orejas R

    更新日期:1997-07-01 00:00:00

  • Minocycline resistance in an oral Streptococcus infantis isolate is encoded by tet(S) on a novel small, low copy number plasmid.

    abstract::We have determined the genetic basis of minocycline resistance in a strain of Streptococcus infantis isolated from a healthy human oral cavity. We demonstrate that tet(S), identical to tet(S) found on the enterococcal conjugative transposon Tn6000, is responsible for the observed resistance. The gene is located on a s...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12410

    authors: Ciric L,Brouwer MS,Mullany P,Roberts AP

    更新日期:2014-04-01 00:00:00

  • Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii.

    abstract::Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02261.x

    authors: Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

    更新日期:2011-06-01 00:00:00

  • Isozyme and morphological characteristics of the anaerobic fungus Piromyces mae isolated from the duodenum, rumen and faeces of sheep.

    abstract::Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07906.x

    authors: Wong MV,Ho YW,Tan SG,Abdullah N,Jalaludin S

    更新日期:1995-12-01 00:00:00

  • Replicating function of the RS1 element associated with Vibrio cholerae CTX phi prophage.

    abstract::The RS1 element associated with Vibrio cholerae CTX phi prophage was cloned from an E1 Tor biotype Vibrio cholerae strain. We used the recA- vaccine strain Peru-15, that lacks the target for RS-mediated site-specific integration, to show that RS1 promotes autonomous replication of a suicide vector. A linker insertion ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13079.x

    authors: Campos J,Fando R,Silva A,Rodriguez BL,Benitez JA

    更新日期:1998-07-01 00:00:00

  • Roundup Ready soybean gene concentrations in field soil aggregate size classes.

    abstract::Roundup Ready (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01449.x

    authors: Levy-Booth DJ,Gulden RH,Campbell RG,Powell JR,Klironomos JN,Pauls KP,Swanton CJ,Trevors JT,Dunfield KE

    更新日期:2009-02-01 00:00:00

  • New genus-specific primers for the PCR identification of novel isolates of the genus Streptomonospora.

    abstract::The halophilic actinomycete genus Streptomonospora, forming a distinct branch in the 16S rRNA gene phylogenetic tree adjacent to the genera Nocardiopsis and Thermobifida, was first proposed by Cui et al. to accommodate the species type Streptomonospora salina. During a biodiversity and taxonomic study on halophilic fi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00402.x

    authors: Zhi XY,Tang SK,Li WJ,Xu LH,Jiang CL

    更新日期:2006-10-01 00:00:00

  • Functional investigation of residue G791 of Escherichia coli 16S rRNA: implication of initiation factor 1 in the restoration of P-site function.

    abstract::Using a specialized ribosome system, previous studies have identified G791 in Escherichia coli 16S rRNA as an invariant and essential residue for ribosome function. To investigate the functional role of G791, we searched for multicopy suppressors that partially restored the protein synthesis ability of mutant ribosome...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02137.x

    authors: Song WS,Ryou SM,Kim HM,Jeon CO,Kim JM,Han SH,Kim SW,Szatkiewicz JP,Cunningham PR,Lee K

    更新日期:2010-12-01 00:00:00

  • Adherence of Candida albicans to components of the subendothelial extracellular matrix.

    abstract::Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(05)80049-7

    authors: Klotz SA

    更新日期:1990-03-15 00:00:00

  • Adherence to host extracellular matrix and serum components by Enterococcus faecium isolates of diverse origin.

    abstract::Enterococcus faecium has emerged as an important cause of nosocomial infections over the last two decades. We recently demonstrated collagen type I (CI) as a common adherence target for some E. faecium isolates and a significant correlation was found to exist between acm-mediated CI adherence and clinical origin. Here...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01806.x

    authors: Zhao M,Sillanpää J,Nallapareddy SR,Murray BE

    更新日期:2009-11-01 00:00:00