The ligase chain reaction distinguishes hepatitis B virus S-gene variants.

abstract：:A succinate semialdehyde dehydrogenase gene (gabD) was identified to be disrupted in a transposon-induced mutant of Ralstonia eutropha exhibiting the phenotype 4-hydroxybutyric acid-leaky. The native gabD gene was cloned by colony hybridization using a homologous gabD-specific DNA probe. DNA sequencing revealed an 145...

journal_title：FEMS microbiology letters

authors： Lütke-Eversloh T,Steinbüchel A

更新日期：1999-12-01 00:00:00

abstract：:In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands remove...

journal_title：FEMS microbiology letters

authors： Stepanova H,Volf J,Malcova M,Matiasovic J,Faldyna M,Rychlik I

更新日期：2011-08-01 00:00:00

abstract：:pH is a key environmental factor affecting the physiology, ecology and pathogenicity of the oral biofilms colonizing the hard tissues of the human mouth. Much attention has been focused on the production of organic acids through the metabolism of carbohydrates by pathogenic oral bacteria. Now, evidence is emerging tha...

journal_title：FEMS microbiology letters

authors： Burne RA,Marquis RE

更新日期：2000-12-01 00:00:00

abstract：:Mixed-substrate conversions are an under-regarded option to fix carbon dioxide in significant amounts. In such a conversion, carbon dioxide together with one other carbon source such as glucose is converted to a single carbon product. With mixed-substrate conversions, it is possible to incorporate carbon dioxide into ...

journal_title：FEMS microbiology letters

authors： Steiger MG,Mattanovich D,Sauer M

更新日期：2017-11-15 00:00:00

abstract：:An open reading frame located in the tyrB-uvrA intergenic region of the Escherichia coli MG1655 chromosome was identified as encoding the class B acid phosphatase of this species on the basis of cloning and expression experiments. A protocol for purification of the enzyme (named AphA) was developed, and its properties...

journal_title：FEMS microbiology letters

authors： Thaller MC,Schippa S,Bonci A,Cresti S,Rossolini GM

更新日期：1997-01-15 00:00:00

abstract：:The devR-devS two-component system of Mycobacterium tuberculosis was identified earlier and partially characterized in our laboratory. A devR::kan mutant of M. tuberculosis was constructed by allelic exchange. The devR mutant strain showed reduced cell-to-cell adherence in comparison to the parental strain in laborato...

journal_title：FEMS microbiology letters

authors： Malhotra V,Sharma D,Ramanathan VD,Shakila H,Saini DK,Chakravorty S,Das TK,Li Q,Silver RF,Narayanan PR,Tyagi JS

更新日期：2004-02-16 00:00:00

abstract：:Natural resistance of wheat plants to wheat sharp eyespot is inadequate, and new strategies for controlling the disease are required. Biological control is an alternative and attractive way of reducing the use of chemicals in agriculture. In this study, we investigated the biocontrol properties of endophytic bacterium...

journal_title：FEMS microbiology letters

authors： Xu YB,Chen M,Zhang Y,Wang M,Wang Y,Huang QB,Wang X,Wang G

更新日期：2014-05-01 00:00:00

abstract：:Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...

journal_title：FEMS microbiology letters

authors： Browne HP,Anvar SY,Frank J,Lawley TD,Roberts AP,Smits WK

更新日期：2015-01-01 00:00:00

abstract：:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

journal_title：FEMS microbiology letters

authors： Kling JJ,Wright RL,Moncrief JS,Wilkins TD

更新日期：1997-01-15 00:00:00

abstract：:The scrA gene coding for sucrose EnzymeII of the phosphoenolpyruvate dependent phosphotransferase system previously isolated from Streptococcus mutans was fused in vitro to the promoterless lacZ' gene to monitor the expression of the scrA gene. The scrA::lacZ gene fusion was introduced back into S. mutans GS-5IS3 by t...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Suzuki R,Kizaki H,Kuramitsu HK

更新日期：1991-04-15 00:00:00

abstract：:Mathematical modeling is an important tool in biological research, allowing for the synthesis of results from many studies into an understanding of a system. Despite this, the need for extensive subject matter knowledge and complex mathematics often leaves modeling as an esoteric subspecialty. A 2-fold approach can be...

journal_title：FEMS microbiology letters

authors： Lofgren ET

更新日期：2016-10-01 00:00:00

abstract：:The Chaetomium globosum strain F0142, which was isolated from barnyard grass, showed potent disease control efficacy against rice blast (Magnaporthe grisea) and wheat leaf rust (Puccinia recondita). Two antifungal substances were purified from broth from this organism and identified as chaetoviridins A and B. Chaetovi...

journal_title：FEMS microbiology letters

authors： Park JH,Choi GJ,Jang KS,Lim HK,Kim HT,Cho KY,Kim JC

更新日期：2005-11-15 00:00:00

abstract：:Roundup Ready (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of ...

journal_title：FEMS microbiology letters

authors： Levy-Booth DJ,Gulden RH,Campbell RG,Powell JR,Klironomos JN,Pauls KP,Swanton CJ,Trevors JT,Dunfield KE

更新日期：2009-02-01 00:00:00

abstract：:The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidat...

journal_title：FEMS microbiology letters

authors： Koyanagi T,Katayama T,Suzuki H,Kumagai H

更新日期：2004-08-01 00:00:00

abstract：:Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

journal_title：FEMS microbiology letters

authors： Ukibe K,Katsuragi T,Tani Y,Takagi H

更新日期：2008-09-01 00:00:00

abstract：:Nitrogenase is oxygen-labile. Cyanobacterial heterocysts can fix N(2) in an oxic milieu because their interior is micro-oxic, for which the glycolipid layer of the heterocyst envelope is required. ORF all5341 of the Anabaena sp. genome predicts a glycosyl transferase. An insertional mutant of all5341 synthesized only ...

journal_title：FEMS microbiology letters

authors： Awai K,Wolk CP

更新日期：2007-01-01 00:00:00

abstract：:Brucella usually carry two highly homologous genes (omp2a and omp2b) for porin-like proteins. In several B. abortus biovars the omp2a gene has a large deletion compared to other Brucella omp2's. In this study we have measured Omp2 pore activity in planar bilayers. Omp2b exhibits well-defined trimeric channel activity ...

journal_title：FEMS microbiology letters

authors： Mobasheri H,Ficht TA,Marquis H,Lea EJ,Lakey JH

更新日期：1997-10-01 00:00:00

abstract：:Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic too...

journal_title：FEMS microbiology letters

authors： Chopra-Dewasthaly R,Marenda M,Rosengarten R,Jechlinger W,Citti C

更新日期：2005-12-01 00:00:00

abstract：:A novel 36-kDa endochitinase named chit36 has been isolated and characterized from Trichoderma harzianum Rifai TM. Partial amino acid sequences from the purified protein were used to clone the fungal cDNA, based on polymerase chain reaction with degenerate primers. The complete open reading frame encodes a 344-amino a...

journal_title：FEMS microbiology letters

authors： Viterbo A,Haran S,Friesem D,Ramot O,Chet I

更新日期：2001-06-25 00:00:00

abstract：:The new thermophilic Bacillus thermoleovorans strain A2 degrades phenol and cresols via the meta cleavage pathway. The first two enzymes involved in this process, the phenol hydroxylase and catechol 2,3-dioxygenase, encoded by the pheA and pheB genes respectively, were cloned and sequenced. The deduced amino acid sequ...

journal_title：FEMS microbiology letters

authors： Duffner FM,Müller R

更新日期：1998-04-01 00:00:00

abstract：:A protease (protease A) was successfully purified from the extracellular proteins of Vibrio parahaemolyticus no. 93, a clinical strain carrying neither tdh nor trh genes, using phenyl-Sepharose CL-4B hydrophobic interaction chromatography. The molecular mass of protease A was 43 kDa using gel filtration, which was in ...

journal_title：FEMS microbiology letters

authors： Lee CY,Cheng MF,Yu MS,Pan MJ

更新日期：2002-03-19 00:00:00

abstract：:When bacterial cells are subjected to stress, cellular and population characteristics can change significantly. Rapid acquisition of these properties of cells in their native physiological state is necessary in order to precisely interpret their survival modes. The multiparametric analytical capability of flow cytomet...

journal_title：FEMS microbiology letters

authors： Sachidanandham R,Gin KY

更新日期：2009-01-01 00:00:00

abstract：:The development of a methodology to identify the origin of fecal pollution is important both for assessing the degree of risk posed to public health and for developing strategies to mitigate the environmental loading of pathogens associated with waterborne disease transmission. Five rep-PCR genomic fingerprinting meth...

journal_title：FEMS microbiology letters

authors： Mohapatra BR,Broersma K,Mazumder A

更新日期：2007-12-01 00:00:00

abstract：:In this report we describe and characterize two oligomer probes that are broadly homologous to conserved eubacterial 16S ribosomal RNA (rRNA) sequences not present in human 18 rRNA or human mitochondrial 12S rRNA. One or both of the probes can detect all of 23 phylogenetically diverse eubacterial nucleic acids against...

journal_title：FEMS microbiology letters

authors： Chen K,Neimark H,Rumore P,Steinman CR

更新日期：1989-01-01 00:00:00

abstract：:The capsular polysaccharide released from the bacterial surface by cell wall turnover during growth exhibited less size heterogeneity and a higher average molecular mass than the polysaccharide extracted from the cell by treatment with lysostaphin or low pH. Treatment of turnover polysaccharide, radiolabelled by growt...

journal_title：FEMS microbiology letters

authors： Havaei SA,Hancock IC

更新日期：1994-05-01 00:00:00

abstract：:A number of hybrids obtained by fusion of protoplasts of complementary auxotrophic strains of Candida albicans were analysed for growth rates, cell volume, DNA content, stability and adherence to human buccal epithelial cells. SDS-PAGE analysis of the cell wall proteins indicated that the hybrid cell walls contained m...

journal_title：FEMS microbiology letters

authors： Law C,Kavanagh K,Whittaker P

更新日期：1994-01-01 00:00:00

abstract：:Bacillus cereus strains involved in food poisoning cases of the diarrheal type may produce two different enterotoxin complexes. To facilitate the identification of hemolysin BL-enterotoxin complex (HBL) and/or the nonhemolytic enterotoxin (NHE) producing colonies a colony immunoblot procedure was developed, which allo...

journal_title：FEMS microbiology letters

authors： Moravek M,Wegscheider M,Schulz A,Dietrich R,Bürk C,Märtlbauer E

更新日期：2004-09-01 00:00:00

abstract：:Haloarchaea thrive under saline and hypersaline conditions and often dominate microbial communities in saltmarshes, salted lakes/soils and some oceanic areas. Some of the predominant species show denitrifying capabilities, although it remains unclear whether they are complete or partial denitrifiers. As complete denit...

journal_title：FEMS microbiology letters

authors： Torregrosa-Crespo J,Bergaust L,Pire C,Martínez-Espinosa RM

更新日期：2018-02-01 00:00:00

abstract：:A search of the complete genome sequence of the halophilic archaeon Halobacterium salinarum failed to identify a gene homologous to the thymidylate synthase (thyA) gene present in the closely related Haloferax volcanii. To understand the source of thymidine synthesis in Hbt. salinarum, a genomic library of Hbt. salina...

journal_title：FEMS microbiology letters

authors： Giladi M,Bitan-Banin G,Mevarech M,Ortenberg R

更新日期：2002-10-29 00:00:00

abstract：:The biosynthesis of glyantrypine from radiolabelled amino acid precursors has been shown experimentally to involve anthranilic acid, tryptophan and glycine. Low values for percentage incorporation of radiolabel into glyantrypine were partly influenced by a complex array of other novel alkaloids shown by the radiolabel...

journal_title：FEMS microbiology letters

authors： Penn J,Purcell M,Mantle PG

更新日期：1992-05-01 00:00:00