The ligase chain reaction distinguishes hepatitis B virus S-gene variants.

Abstract:

:A novel method for the identification of hepatitis B virus (HBV) variants was developed. The ligase chain reaction (LCR) distinguished the sequences of two isolates from the Gambia showing a change at nucleotide 421 within the region coding for the surface protein a antigenic determinant. One sequence was derived from a child previously immunized with HBV vaccine, while the other, reported here for the first time, was from a chronic carrier. Nucleotide variations within the target sequence at, or up to 3 bases from the point of ligation inhibited the reaction. The LCR recognised variations in as little as 0.9 fmol DNA and will permit the rapid detection of HBV variants in molecular epidemiological studies.

journal_name

FEMS Microbiol Lett

authors

Karthigesu VD,Mendy M,Fortuin M,Whittle HC,Howard CR,Allison LM

doi

10.1111/j.1574-6968.1995.tb07766.x

subject

Has Abstract

pub_date

1995-09-01 00:00:00

pages

127-32

issue

2

eissn

0378-1097

issn

1574-6968

pii

0378-1097(95)00245-Z

journal_volume

131

pub_type

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