The ligase chain reaction distinguishes hepatitis B virus S-gene variants.


:A novel method for the identification of hepatitis B virus (HBV) variants was developed. The ligase chain reaction (LCR) distinguished the sequences of two isolates from the Gambia showing a change at nucleotide 421 within the region coding for the surface protein a antigenic determinant. One sequence was derived from a child previously immunized with HBV vaccine, while the other, reported here for the first time, was from a chronic carrier. Nucleotide variations within the target sequence at, or up to 3 bases from the point of ligation inhibited the reaction. The LCR recognised variations in as little as 0.9 fmol DNA and will permit the rapid detection of HBV variants in molecular epidemiological studies.


FEMS Microbiol Lett


Karthigesu VD,Mendy M,Fortuin M,Whittle HC,Howard CR,Allison LM




Has Abstract


1995-09-01 00:00:00














  • Correlation between the effects of fosfomycin and chloramphenicol on Escherichia coli.

    abstract::It was speculated that the increase of the UDP-GlcNAc pool observed with chloramphenicol can modulate the residual PEP:UDP-GlcNAc-enolpyruvate activity of fosfomycin-treated cells. This provided an explanation on how chloramphenicol can insure the formation of enough UDP-MurNAc-pentapeptide to sustain peptidoglycan sy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mengin-Lecreulx D,van Heijenoort J

    更新日期:1990-01-01 00:00:00

  • The bifunctional NadR regulator of Salmonella typhimurium: location of regions involved with DNA binding, nucleotide transport and intramolecular communication.

    abstract::NadR is the repressor protein that controls the expression of genes for NAD synthesis. It is also believed to be involved in nucleotide transport. Point mutations conferring different phenotypes were localized to six different regions within the nadR gene. That mutations affecting repression and transport all mapped w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Foster JW,Penfound T

    更新日期:1993-09-01 00:00:00

  • Characterization of pRAS1-like plasmids from atypical North American psychrophilic Aeromonas salmonicida.

    abstract::Atypical psychrophilic Aeromonas salmonicida isolates were obtained from farmed and wild fish in Northeastern North America. These bacteria were isolated between 1992 and 2001 and carried tetracycline resistance (Tc(r)) plasmids of approximately 58 kb. The nine isolates had plasmids which could be divided into four gr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Casas C,Anderson EC,Ojo KK,Keith I,Whelan D,Rainnie D,Roberts MC

    更新日期:2005-01-01 00:00:00

  • Infection by the 'photosynthetic' phage S-PM2 induces increased synthesis of phycoerythrin in Synechococcus sp. WH7803.

    abstract::Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shan J,Jia Y,Clokie MR,Mann NH

    更新日期:2008-06-01 00:00:00

  • Use of lectins for a comparative study of cell wall composition of different anaerobic rumen fungal strains.

    abstract::The technique based on fluorescein-linked lectins used to determine the cell wall structure of anaerobic rumen fungi belonging to genera: Neocallimastix, Piromonas and Sphaeromonas, appears to be an interesting tool for distinguishing between strains. Furthermore this technique shows differences of cell wall compositi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Guillot J,Breton A,Damez M,Dusser M,Gaillard-Martinie B,Millet L

    更新日期:1990-01-15 00:00:00

  • Novel plasmids in clinical strains of Streptococcus pneumoniae.

    abstract::Small plasmids were found in two clinical isolates of Streptococcus pneumoniae from Spain (strains 671 and 678) and in one strain (SpR) isolated in Germany. All three strains contained one plasmid (2.75 to 3.1 kb) which is related to the only previously described pneumococcal plasmid, pDP1. Strains 678 and SpR carried...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sibold C,Markiewicz Z,Latorre C,Hakenbeck R

    更新日期:1991-01-01 00:00:00

  • Is the fur gene of Rhizobium leguminosarum essential?

    abstract::Using primers corresponding to conserved regions of the bacterial regulatory gene fur, a homologue of this gene from the genome of Rhizobium leguminosarum biovar viciae, the nitrogen-fixing symbiont of peas, was isolated and sequenced. The fur gene is normally expressed constitutively, independent of the presence of F...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Luca NG,Wexler M,Pereira MJ,Yeoman KH,Johnston AW

    更新日期:1998-11-15 00:00:00

  • Mercury-resistant actinomycetes from the Chesapeake Bay.

    abstract::Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ravel J,Amoroso MJ,Colwell RR,Hill RT

    更新日期:1998-05-01 00:00:00

  • Characterization of the SOS response of Pseudomonas fluorescens strain DC206 using whole-genome transcript analysis.

    abstract::DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jin H,Retallack DM,Stelman SJ,Hershberger CD,Ramseier T

    更新日期:2007-04-01 00:00:00

  • Roundup Ready soybean gene concentrations in field soil aggregate size classes.

    abstract::Roundup Ready (RR) soybeans containing recombinant Agrobacterium spp. CP4 5-enol-pyruvyl-shikimate-3-phosphate synthase (cp4 epsps) genes tolerant to the herbicide glyphosate are extensively grown worldwide. The concentration of recombinant DNA from RR soybeans in soil aggregates was studied due to the possibility of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Levy-Booth DJ,Gulden RH,Campbell RG,Powell JR,Klironomos JN,Pauls KP,Swanton CJ,Trevors JT,Dunfield KE

    更新日期:2009-02-01 00:00:00

  • Analysis of Cre1 binding sites in the Trichoderma reesei cbh1 upstream region.

    abstract::A 1.5-kb XbaI-SacII fragment containing the upstream region of the Trichoderma reesei cellobiohydrolase I gene (cbh1) has been sequenced. The 1.5-kb fragment contains eight 6-bp sites having an identical or similar sequence to the consensus sequence for binding a catabolite repressor, Aspergillus nidulans CreA. Result...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Takashima S,Iikura H,Nakamura A,Masaki H,Uozumi T

    更新日期:1996-12-15 00:00:00

  • Molecular mechanism of MexR-mediated regulation of MexAB-OprM efflux pump expression in Pseudomonas aeruginosa.

    abstract::To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction, and transcription of the mexA-lacZ reporter gene containing different lengths of mexOP. Homogeneously purified MexR bound speci...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Saito K,Eda S,Maseda H,Nakae T

    更新日期:2001-02-05 00:00:00

  • Identification Leptospira santarosai serovar shermani specific sequences by suppression subtractive hybridization.

    abstract::In Taiwan, leptospirosis is caused mainly by Leptospira santarosai serovar shermani. Suppression subtractive hybridization was employed to isolate DNA fragments present in pathogenic L. santarosai serovar shermani but absent in non-pathogenic L. biflexa serovar patoc. Analysis of 23 subtracted DNA clones revealed 25 g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hsieh WJ,Pan MJ

    更新日期:2004-06-01 00:00:00

  • Towards biobased industry: acetate as a promising feedstock to enhance the potential of microbial cell factories.

    abstract::A broad range of different chemical and pharmaceutical compounds have been produced in microbial cell factories. To compete with traditional crude oil based production processes, the use of complex alternative raw materials such as lignocellulosic biomass, waste streams and utilization of CO2 in gas fermentations has ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Novak K,Pflügl S

    更新日期:2018-10-01 00:00:00

  • Modulation of the fecal microbiota by the intake of a Lactobacillus johnsonii La1-containing product in human volunteers.

    abstract::Lactobacillus johnsonii La1 (La1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. This study evaluates how the ingestion of different amounts of La1 influences the main bacterial populations of the fecal microbiota. Eight asymptomatic voluntee...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Garrido D,Suau A,Pochart P,Cruchet S,Gotteland M

    更新日期:2005-07-15 00:00:00

  • Magnaporthe oryzae endopolygalacturonase homolog correlates with density-dependent conidial germination.

    abstract::Endopolygalacturonases (endoPGs) of some phytopathogens are virulent factors for dicots. To investigate the function of the endoPG of Magnaporthe oryzae, a disruption mutant of MGG_08938, the homolog of endoPG found in the genome database of this fungus, was generated. The pathogenicity, mycelial growth, and appressor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mori T,Jung HY,Maejima K,Hirata H,Himeno M,Hamamoto H,Namba S

    更新日期:2008-03-01 00:00:00

  • Isolation of Rhodococcus rhodochrous NCIMB13064 derivatives with new biodegradative abilities.

    abstract::Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chlor...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kulakova AN,Reid KA,Larkin MJ,Allen CC,Kulakov LA

    更新日期:1996-12-01 00:00:00

  • Isolation of a novel plasmid, pNi15, from Enterobacter sp. Ni15 containing a nickel resistance gene.

    abstract::We isolated nickel-resistant bacterium from soil in order to identify a novel nickel resistance determinant. Using 16S rRNA gene sequencing, an isolate was identified as Enterobacter sp. Ni15. This species showed a medium-level (resistant to up to 10 mM) nickel resistance in nutrient-rich media. Enterobacter sp. Ni15 ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Lee YK,Chang HH,Lee HJ,Park H,Lee KH,Joe MH

    更新日期:2006-04-01 00:00:00

  • Efficient screening for astaxanthin-overproducing mutants of the yeast Xanthophyllomyces dendrorhous by flow cytometry.

    abstract::Astaxanthin possesses higher antioxidant activity than other carotenoids and, for this and other reasons, has great commercial potential for use in the aquaculture, pharmaceutical, and food industries. The basidiomycetous yeast Xanthophyllomyces dendrorhous is one of the best natural producers of astaxanthin, but wild...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ukibe K,Katsuragi T,Tani Y,Takagi H

    更新日期:2008-09-01 00:00:00

  • Influence of carbon monoxide on metabolite formation in Methanosarcina acetivorans.

    abstract::Methanogenic archaea conserve energy for growth by reducing some one- and two-carbon compounds to methane and concomitantly generating an ion motive force. Growth of Methanosarcina acetivorans on carbon monoxide (CO) is peculiar as it involves formation of, besides methane, formate, acetate and methylated thiols. It h...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Oelgeschläger E,Rother M

    更新日期:2009-03-01 00:00:00

  • Isolation of insertion elements from gram-positive Brevibacterium, Corynebacterium and Rhodococcus strains using the Bacillus subtilis sacB gene as a positive selection marker.

    abstract::The sacB gene of Bacillus subtilis was successfully applied in various Arthrobacter, Brevibacterium, Corynebacterium and Rhodococcus strains for the isolation of transposable elements. Three different insertion sequence (IS) elements entrapped in sacB were isolated. The IS elements IS-Bl and IS-Cg isolated from Brevib...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jäger W,Schäfer A,Kalinowski J,Pühler A

    更新日期:1995-02-01 00:00:00

  • Role of the Talaromyces marneffei (Penicillium marneffei) sakA gene in nitrosative stress response, conidiation and red pigment production.

    abstract::Stress-activated MAPK pathways are systems used to regulate the stress adaptation of most fungi. It has been shown that in Talaromyces marneffei (Penicillium marneffei), a pathogenic dimorphic fungus, the sakA gene is involved, not only in tolerance against oxidative and heat stresses, but also in playing a role in as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nimmanee P,Tam EWT,Woo PCY,Vanittanakom P,Vanittanakom N

    更新日期:2017-04-01 00:00:00

  • Bacterial triterpenoids of the hopane series as biomarkers for the chemotaxonomy of Burkholderia, Pseudomonas and Ralstonia spp.

    abstract::Hopanoid fingerprints allowed to differentiate bacteria formerly connected to the genus Pseudomonas. Whereas all strains related to Pseudomonas and Ralstonia were devoid of any detectable hopanoid, these pentacyclic triterpenoids were found in the Burkholderia species and in related soil isolates, which contained as m...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cvejic JH,Putra SR,El-Beltagy A,Hattori R,Hattori T,Rohmer M

    更新日期:2000-02-15 00:00:00

  • Identification of a Ferric uptake regulator from Microcystis aeruginosa PCC7806.

    abstract::Ferric uptake regulator (Fur) proteins are widely recognized as repressors that in many prokaryotes regulate a large number of genes involved in iron homeostasis and oxidative stress response. In our study, we were able to identify the complete sequence of the fur gene from Microcystis aeruginosa using inverse-polymer...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Martin-Luna B,Hernandez JA,Bes MT,Fillat MF,Peleato ML

    更新日期:2006-01-01 00:00:00

  • Purification and properties of an esterase from Aspergillus nomius HS-1 degrading ethylene glycol dibenzoate.

    abstract::We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electroph...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Uchida H,Kurakata Y,Sawamura H,Inamura N,Kotani T,Uwajima T

    更新日期:2003-06-06 00:00:00

  • Kluyveromyces wickerhamii killer toxin: purification and activity towards Brettanomyces/Dekkera yeasts in grape must.

    abstract::Brettanomyces/Dekkera yeasts have been identified as part of the grape yeast flora. They are well known for colonizing the cellar environmental and spoiling wines, causing haze, turbidity and strong off-flavours in wines and enhancing the volatile acidity. As the general practices applied to combat Brettanomyces/Dekke...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Comitini F,Ciani M

    更新日期:2011-03-01 00:00:00

  • Denaturing gradient gel electrophoresis gel expansion (DGGEGE)--an attempt to resolve the limitations of co-migration in the DGGE of complex polymicrobial communities.

    abstract::Recent molecular approaches for the study of microbial communities such as PCR-cloning have enabled the detection and identification of as-yet-unculturable taxa. Cloning and sequencing of multiple samples is extremely laborious and expensive to perform thoroughly due to the large diversity involved. For this purpose, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gafan GP,Spratt DA

    更新日期:2005-12-15 00:00:00

  • Alternative strategies for lignocellulose fermentation through lactic acid bacteria: the state of the art and perspectives.

    abstract::Lactic acid bacteria (LAB) have a long history in industrial processes as food starters and biocontrol agents, and also as producers of high-value compounds. Lactic acid, their main product, is among the most requested chemicals because of its multiple applications, including the synthesis of biodegradable plastic pol...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审


    authors: Tarraran L,Mazzoli R

    更新日期:2018-08-01 00:00:00

  • Biological activity of the azlactone derivative EPA-35 against Trypanosoma cruzi.

    abstract::Chagas disease, caused by Trypanosoma cruzi, affects six to seven million people worldwide. Treatment is based on benznidazole, producing several side effects and debatable efficacy, highlighting the need for new alternative drugs. We investigated the activity of four C-4 functionalized azlactone derivatives (EPA-27, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: de Azeredo CM,Ávila EP,Pinheiro DL,Amarante GW,Soares MJ

    更新日期:2017-02-01 00:00:00

  • Cloning and analysis of the polyhydroxyalkanoic acid synthase gene from an Acinetobacter sp.: evidence that the gene is both plasmid and chromosomally located.

    abstract::The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Schembri MA,Bayly RC,Davies JK

    更新日期:1994-05-01 00:00:00