The LIV-I/LS system as a determinant of azaserine sensitivity of Escherichia coli K-12.

Abstract:

:The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidated except that all the azaserine-resistant strains isolated so far carry lesions in the aroP gene as a primary determinant. Here, we demonstrate that, in addition to AroP, the LIV-I/LS system, an ATP-binding cassette type transporter, is involved in azaserine sensitivity of E. coli, by genetic analysis and transport studies, in which Ki value for azaserine was determined to be approximately 10(-3) M.

journal_name

FEMS Microbiol Lett

authors

Koyanagi T,Katayama T,Suzuki H,Kumagai H

doi

10.1016/j.femsle.2004.06.017

keywords:

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

73-7

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378109704004367

journal_volume

237

pub_type

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