Abstract:
:The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidated except that all the azaserine-resistant strains isolated so far carry lesions in the aroP gene as a primary determinant. Here, we demonstrate that, in addition to AroP, the LIV-I/LS system, an ATP-binding cassette type transporter, is involved in azaserine sensitivity of E. coli, by genetic analysis and transport studies, in which Ki value for azaserine was determined to be approximately 10(-3) M.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Koyanagi T,Katayama T,Suzuki H,Kumagai Hdoi
10.1016/j.femsle.2004.06.017keywords:
subject
Has Abstractpub_date
2004-08-01 00:00:00pages
73-7issue
1eissn
0378-1097issn
1574-6968pii
S0378109704004367journal_volume
237pub_type
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