Abstract:
:A total of 129 sRNA candidates were identified in Brucella abortus 2308 in our previous work, and one candidate with potential to regulate expression of hemH gene was further analyzed in this study. We found that the novel sRNA can inhibit the expression of hemH and called it BsrH (Brucella sRNA regulating HemH). The expression level of BsrH was tested in four different stress conditions. A significant upregulation was detected during the growth in acidic and Brucella minimal media, as well as in the presence of hydroxyl peroxide, while iron deficiency caused the opposite effect. As expected, BsrH strongly affected the survival ratio of the Brucella cells under iron-limitation conditions, though overexpression of BsrH did not affect Brucella virulence. Thus, we conclude that BsrH plays a regulatory role in bacterial heme biosynthesis and can be considered as the first Brucella sRNA involved in stress responses.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Peng X,Dong H,Wu Qdoi
10.1093/femsle/fnu017subject
Has Abstractpub_date
2015-01-01 00:00:00pages
1-7issue
2eissn
0378-1097issn
1574-6968pii
fnu017journal_volume
362pub_type
杂志文章abstract::An iterative position-specific score matrix (PSSM)-based approach was used to predict sigma(28) promoters in 11 Shewanella genomes. The Shewanella Correlation Browser was used to distinguish true-positive predictions from false-positive predictions in Shewanella oneidensis MR-1 by generating a sigma(28)-regulated tran...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.2001.tb10638.x
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1997.tb10460.x
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1993.tb06360.x
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1996.tb08548.x
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journal_title:FEMS microbiology letters
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abstract::The locus of enterocyte effacement pathogenicity island confers the attaching and effacing histopathology on epithelial cells infected with enteropathogenic and enterohemorrhagic Escherichia coli. We investigated the site of insertion of the locus of enterocyte effacement in E. coli strains in relation to their evolut...
journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1997.tb12704.x
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abstract::DNA polymerase from the archaeon Pyrococcus abyssi strain Orsay was expressed in Escherichia coli. The recombinant DNA polymerase (Pab) was purified to homogeneity by heat treatment followed by 5 steps of chromatography and characterized for PCR applications. Buffer optimization experiments indicated that Pab PCR perf...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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doi:10.1016/j.femsle.2004.06.041
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.2007.00744.x
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journal_title:FEMS microbiology letters
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更新日期:2008-04-01 00:00:00
abstract::Legionella pneumophila, the aetiologic agent of legionnaires' disease, contains a single, monopolar flagellum which is composed of one major subunit, the FlaA protein. Expression studies using a reporter gene fusion of the flaA promoter with the luxAB gene revealed that the flaA expression is not only temperature regu...
journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1999.tb13603.x
更新日期:1999-06-01 00:00:00
abstract::The expression of nifA-, niH- and nifB-lacZ fusions was examined in different mutants of Azospirillum brasilense. Mutations in nifA, glnA and glnB severely impaired the expression of nifH- and nifB-lacZ fusions. By contrast, a nifA-lacZ fusion was not affected in a nifA or a glnB background and was only partially impa...
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doi:10.1111/j.1574-6968.1992.tb14028.x
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1995.tb07511.x
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1997.tb10249.x
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journal_title:FEMS microbiology letters
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abstract::Streptococcus suis serotype 2 (S. suis 2 or SS2) is the causative agent of several diseases in both pigs and humans. 6-Phosphogluconate dehydrogenase (6PGD) is a cell surface protein in SS2. In this study, the immunogenicity and protective efficacy of recombinant 6PGD (r6PGD) from SS2 was evaluated in piglets. Immuniz...
journal_title:FEMS microbiology letters
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更新日期:2009-07-01 00:00:00
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journal_title:FEMS microbiology letters
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doi:10.1016/j.femsle.2004.12.030
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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doi:10.1111/j.1574-6968.1999.tb13495.x
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
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更新日期:2017-08-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1990.tb13994.x
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abstract::The 181 251 bp accessory plasmid pSmeSM11b of Sinorhizobium meliloti strain SM11, belonging to a dominant indigenous S. meliloti subpopulation identified during a long-term field release experiment, was sequenced. This plasmid has 166 coding sequences (CDSs), 42% of which encode proteins with homology to proteins of k...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2007.00731.x
更新日期:2007-06-01 00:00:00