Conservation of surface epitopes in Pseudomonas aeruginosa outer membrane porin protein OprF.

Abstract:

:The outer membrane proteins of several prominent bacterial pathogens demonstrate substantial variation in their surface antigenic epitopes. To determine if this was also true for Pseudomonas aeruginosa outer membrane protein OprF, gene sequencing of a serotype 5 isolate was performed to permit comparison with the published serotype 12 oprF gene sequence. Only 16 nucleotide substitutions in the 1053 nucleotide coding region were observed; none of these changed the amino acid sequence. A panel of 10 monoclonal antibodies (mAbs) reacted with each of 46 P. aeruginosa strains representing all 17 serotype strains, 12 clinical isolates, 15 environmental isolates and 2 laboratory isolates. Between two and eight of these mAbs also reacted with proteins from representatives of the rRNA homology group I of the Pseudomonadaceae. Nine of the ten mAbs recognized surface antigenic epitopes as determined by indirect immunofluorescence techniques and their ability to opsonize P. aeruginosa for phagocytosis. These epitopes were partially masked by lipopolysaccharide side chains as revealed using a side chain-deficient mutant. It is concluded that OprF is a highly conserved protein with several conserved surface antigenic epitopes.

journal_name

FEMS Microbiol Lett

authors

Martin NL,Rawling EG,Wong RS,Rosok M,Hancock RE

doi

10.1111/j.1574-6968.1993.tb06524.x

subject

Has Abstract

pub_date

1993-11-01 00:00:00

pages

261-6

issue

3

eissn

0378-1097

issn

1574-6968

journal_volume

113

pub_type

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