SEF17 fimbriae are essential for the convoluted colonial morphology of Salmonella enteritidis.

Abstract:

:Salmonella enteritidis isolated from poultry infections generated a convoluted colonial morphology after 48 h growth on colonisation factor antigen (CFA) agar at 25 degrees C. A mutant S. enteritidis defective for the elaboration of the SEF17 fimbrial antigen, in which the agf gene cluster was inactivated by insertion of an ampicillin resistance gene cassette, and other wild-type S. enteritidis transduced to this genotype failed to produce convoluted colonies. However, growth of SEF17- mutants at 25 degrees C on CFA agar supplemented with 0.001% Congo red resulted in partial recovery of the phenotype. Immunoelectron microscopy demonstrated that copious amounts of the SEF17 fimbrial antigen were present in the extracellular matrix of convoluted colonies of wild-type virulent S. enteritidis isolates. Bacteria were often hyperflagellated also. Immunoelectron microscopy of SEF17- mutants grown on CFA agar+0.001% Congo red demonstrated the elaboration of an as yet undefined fimbrial structure. Isolates of S. enteritidis which were described previously as avirulent and sensitive to environmental stress failed to express SEF17 or produce convoluted colonies. These data indicate an essential role for SEF17, and possibly for another fimbria and flagella, in the generation of the convoluted colonial phenotype. The relationship between virulence and colonial phenotype is discussed.

journal_name

FEMS Microbiol Lett

authors

Allen-Vercoe E,Dibb-Fuller M,Thorns CJ,Woodward MJ

doi

10.1111/j.1574-6968.1997.tb10460.x

subject

Has Abstract

pub_date

1997-08-01 00:00:00

pages

33-42

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(97)00219-X

journal_volume

153

pub_type

杂志文章
  • The activity of bacteriocins from Carnobacterium maltaromaticum UAL307 against gram-negative bacteria in combination with EDTA treatment.

    abstract::Bacteriocins from gram-positive bacteria are potent antimicrobial peptides that inhibit pathogenic and food-spoilage bacteria. They are usually ineffective against gram-negative bacteria because they cannot penetrate the outer membrane (OM). Disruption of the OM of some gram-negative bacteria was reported to sensitize...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02223.x

    authors: Martin-Visscher LA,Yoganathan S,Sit CS,Lohans CT,Vederas JC

    更新日期:2011-04-01 00:00:00

  • Induction of a viable but non-culturable state in Salmonella Typhimurium by thermosonication and factors affecting resuscitation.

    abstract::The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx249

    authors: Liao H,Jiang L,Zhang R

    更新日期:2018-02-01 00:00:00

  • Characteristics of Streptomyces griseus biofilms in continuous flow tubular reactors.

    abstract::The purpose of this study was to investigate the feasibility of cultivating the biotechnologically important bacterium Streptomyces griseus in single-species and mixed-species biofilms using a tubular biofilm reactor (TBR). Streptomyces griseus biofilm development was found to be cyclical, starting with the initial ad...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/1574-6968.12378

    authors: Winn M,Casey E,Habimana O,Murphy CD

    更新日期:2014-03-01 00:00:00

  • Morphological and intracellular alterations induced by cytotoxin VT2y produced by Escherichia coli isolated from chickens with swollen head syndrome.

    abstract::Recently, a novel verocytotoxin named VT2y was described which belongs to the STx family and is produced by Escherichia coli isolated from domestic poultry with swollen head syndrome (SHS). The VT2y toxin induced apoptosis in Vero, HeLa, CHO, CEF (primary chicken embryo fibroblast) and PCK (primary chicken kidney) cel...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10586.x

    authors: Salvadori MR,Yamada AT,Yano T

    更新日期:2001-04-01 00:00:00

  • Transcription and genetic analyses of a putative N-acetylmuramyl-L-alanine amidase in Borrelia burgdorferi.

    abstract::In this study, a putative N-acetylmuramyl-l-alanine amidase gene (bb0666) was identified in the genome of the Lyme disease spirochete Borrelia burgdorferi. This protein shares c. 30% identity with its counterparts from other bacteria. Reverse transcriptase-PCR analysis showed that bb0666 along with two other genes (bb...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01416.x

    authors: Yang Y,Li C

    更新日期:2009-01-01 00:00:00

  • Identification of the transcription factor responsible for L-malate-dependent regulation in the marine Antarctic bacterium Pseudoalteromonas haloplanktis TAC125.

    abstract::Two-component systems are widespread in nature and constitute the most common mechanism of transmembrane signal transduction in bacteria. Recently, a functionally active two-component system consisting of malS and malR genes possibly involved in the expression of a C4-dicarboxylate transporter system (dctAB operon) wa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01589.x

    authors: Papa R,Rippa V,Duilio A

    更新日期:2009-06-01 00:00:00

  • Increased expression and hotspot mutations of the multidrug efflux transporter, CDR1 in azole-resistant Candida albicans isolates from vaginitis patients.

    abstract::The aims of our research were to investigate the gene expression of the multidrug efflux transporter, CDR1 and the major drug facilitator superfamily transporter, MDR1 gene in azole drug-resistant Candida albicans and Candida glabrata clinical isolates recovered from vaginitis patients; and to identify hotspot mutatio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.06.036

    authors: Looi CY,D' Silva EC,Seow HF,Rosli R,Ng KP,Chong PP

    更新日期:2005-08-15 00:00:00

  • Role of oxidative stress and mitochondrial changes in cyanobacteria-induced apoptosis and hepatotoxicity.

    abstract::Microcystins produced by cyanobacteria are potent and specific hepatotoxins; however, the mechanisms of microcystin-induced hepatotoxicity have not been fully elucidated. The induction of free radical formation and mitochondrial alterations are two major events found in microcystin-treated cultured rat hepatocytes. Th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1016/S0378-1097(03)00100-9

    authors: Ding WX,Nam Ong C

    更新日期:2003-03-14 00:00:00

  • Biological activity of the azlactone derivative EPA-35 against Trypanosoma cruzi.

    abstract::Chagas disease, caused by Trypanosoma cruzi, affects six to seven million people worldwide. Treatment is based on benznidazole, producing several side effects and debatable efficacy, highlighting the need for new alternative drugs. We investigated the activity of four C-4 functionalized azlactone derivatives (EPA-27, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx020

    authors: de Azeredo CM,Ávila EP,Pinheiro DL,Amarante GW,Soares MJ

    更新日期:2017-02-01 00:00:00

  • Rhizobium leguminosarum bv. trifolii rosR gene expression is regulated by catabolic repression.

    abstract::Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis. Transcription of rosR is directed by two promoters, distal P1 and proximal P2, of different strengths. We demonstrated that rosR P1 functions as the main promoter and, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01443.x

    authors: Janczarek M,Skorupska A

    更新日期:2009-02-01 00:00:00

  • Expression of green fluorescent protein in Bacillus brevis under the control of a novel constitutive promoter F1 and insertion mutagenesis of F1 in Escherichia coli DH5alpha.

    abstract::The constitutive expression vector pHY300-F1gfp was constructed to test the function of a promoter, F1, cloned from the rice epiphyte Bacillus brevis strain DX01. The DX01 cells harboring the plasmid pHY300-F1gfp were shown to produce bright green fluorescence. The results were confirmed by Western blot analysis and f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00797-3

    authors: Chen Y,Yan J,Yang M,Wang J,Shen D

    更新日期:2003-12-05 00:00:00

  • Antibiotic resistance in oral commensal streptococci from healthy Mexicans and Cubans: resistance prevalence does not mirror antibiotic usage.

    abstract::Antibiotic resistance genes might be maintained by selection pressures different from those which are responsible for initially selecting resistant bacteria. This possibility was suggested from a comparison of oral commensal streptococci isolated from healthy people not taking antibiotics. Resistance frequencies were ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11471.x

    authors: Díaz-Mejía JJ,Carbajal-Saucedo A,Amábile-Cuevas CF

    更新日期:2002-12-17 00:00:00

  • Denitrification by yeasts and occurrence of cytochrome P450nor in Trichosporon cutaneum.

    abstract::Yeasts of various genera were screened for denitrifying activity, and several yeast strains such as Trichosporon cutaneum, Fellomyces fuzhouensis, and Candida sp. were found to exhibit distinct activities to convert nitrite to nitrous oxide. Dissimilatory nitrite reductase (Nir) or nitric oxide reductase (Nor) activit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13262.x

    authors: Tsuruta S,Takaya N,Zhang L,Shoun H,Kimura K,Hamamoto M,Nakase T

    更新日期:1998-11-01 00:00:00

  • Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii.

    abstract::Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02261.x

    authors: Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

    更新日期:2011-06-01 00:00:00

  • Screening and analysis of DNA fragments that show promoter activities in Thermus thermophilus.

    abstract::We have constructed a Thermus promoter probe vector pPP11 containing the promoterless heat-stable kanamycin resistance (Kmr) gene. Total DNA of T. thermophilus HB27 was randomly sheared into 100-200 bp fragments and inserted into the EcoRI site of pPP11. About 1000 clones which showed Kmr were obtained, and they were ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07511.x

    authors: Maseda H,Hoshino T

    更新日期:1995-05-01 00:00:00

  • An extra large insertion in the polyhydroxyalkanoate synthase from Delftia acidovorans DS-17: its deletion effects and relation to cellular proteolysis.

    abstract::The polyhydroxyalkanoate (PHA) synthase (PhaC(Da)) from Delftia acidovorans DS-17 (formerly Comamonas acidovorans) has a unique large insertion consisting of 40 amino acid residues in the alpha/beta hydrolase fold region. In order to examine whether this insertion is necessary for enzyme function, we generated a mutan...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00930-3

    authors: Tsuge T,Imazu S,Takase K,Taguchi S,Doi Y

    更新日期:2004-02-09 00:00:00

  • Predicting sigma28 promoters in eleven Shewanella genomes.

    abstract::An iterative position-specific score matrix (PSSM)-based approach was used to predict sigma(28) promoters in 11 Shewanella genomes. The Shewanella Correlation Browser was used to distinguish true-positive predictions from false-positive predictions in Shewanella oneidensis MR-1 by generating a sigma(28)-regulated tran...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01175.x

    authors: Song W,Juhn FS,Naiman DQ,Konstantinidis KT,Gardner TS,Ward MJ

    更新日期:2008-06-01 00:00:00

  • Proteomics and the stressful life of lactobacilli.

    abstract::Lactobacilli occupy specific ecological niches, where they represent a major component of foods, human and animal microbial communities. Employing these bacteria in industrial fermentations or for human health benefits exposes them to certain life-threatening conditions where their ability to adapt plays a key role in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/1574-6968.12274

    authors: Hussain MA,Hosseini Nezhad M,Sheng Y,Amoafo O

    更新日期:2013-12-01 00:00:00

  • The LIV-I/LS system as a determinant of azaserine sensitivity of Escherichia coli K-12.

    abstract::The growth of Escherichia coli is inhibited by an antibiotic compound, azaserine (O-diazoacetyl-L-serine). Previous studies revealed the biochemical properties of azaserine, which involves inhibition of various enzymatic reactions as well as introduction of DNA breakage. However, genetically, nothing has been elucidat...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.06.017

    authors: Koyanagi T,Katayama T,Suzuki H,Kumagai H

    更新日期:2004-08-01 00:00:00

  • Isolation and partial characterization of a compound with siderophore activity from Vibrio parahaemolyticus.

    abstract::A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compou...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90605-n

    authors: Yamamoto S,Fujita Y,Okujo N,Minami C,Matsuura S,Shinoda S

    更新日期:1992-07-01 00:00:00

  • Effect of Mg(2+) ion in protein secretion by magnesium-resistant strains of Pseudomonas aeruginosa and Vibrio parahaemolyticus isolated from the coastal water of Haldia port.

    abstract::A rapidly growing industrial complex including oil refineries and chemical industries has developed around the coastal area of Haldia port in the district of Midnapore, West Bengal, India. The coastal water is highly polluted with industrial wastes along with petroleum hydrocarbons. The bacteria isolated from the diff...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09054.x

    authors: Bhattacharya M,Roy SS,Biswas D,Kumar R

    更新日期:2000-04-15 00:00:00

  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions.

    abstract::In artificial engineered systems, nitrification is a key reaction that accounts for the removal of biological nitrogen. Recently, a single microbe capable of oxidizing ammonia to nitrate, known as a complete ammonia oxidizer (comammox), has been discovered. Although the abundance and diversity of comammox Nitrospira i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnaa025

    authors: Fujitani H,Nomachi M,Takahashi Y,Hasebe Y,Eguchi M,Tsuneda S

    更新日期:2020-01-01 00:00:00

  • RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion.

    abstract::It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00838.x

    authors: Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

    更新日期:2007-09-01 00:00:00

  • Ku70 or Ku80 deficiencies in the fungus Botrytis cinerea facilitate targeting of genes that are hard to knock out in a wild-type context.

    abstract::The filamentous ascomycete Botrytis cinerea is one of the most studied models for understanding the necrotrophic behaviour of phytopathogenic fungi. The genomes of two strains of B. cinerea have been sequenced (B05.10 and T4), which may contribute to elucidating the virulence polymorphism in this fungus. In this study...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01388.x

    authors: Choquer M,Robin G,Le Pêcheur P,Giraud C,Levis C,Viaud M

    更新日期:2008-12-01 00:00:00

  • Diversity of chromosomal AmpC beta-lactamases from Enterobacter cloacae isolates in a Portuguese hospital.

    abstract::Six clinical isolates of Enterobacter cloacae isolated in a Portuguese hospital, between April 1999 and November 2000, demonstrated resistance to almost all broad-spectrum cephalosporins, except to cefepime. These isolates were susceptible to quinolones and to aminoglycosides. Isoelectric focusing demonstrated product...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00891-7

    authors: Conceição T,Faria N,Lito L,Melo Cristino J,Salgado MJ,Duarte A

    更新日期:2004-01-30 00:00:00

  • The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis.

    abstract::Hydrogenobacter acidophilus strain 3H-1 is a thermoacidophilic, obligately chemolithoautotrophic, hydrogen-oxidizer isolated from a Japanese solfataric field. Strain 3H-1 requires elemental sulfur for growth. We used PCR to amplify the 16S rRNA gene of strain 3H-1, and sequenced the amplification product directly. Phy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06877.x

    authors: Shima S,Yanagi M,Saiki H

    更新日期:1994-06-01 00:00:00

  • Multiplicity and expression of xylanases in the rumen fungus Neocallimastix frontalis.

    abstract::The time course production of xylanolytic enzymes by the rumen anaerobic fungus Neocallimastix frontalis was studied during growth on different carbon sources and revealed using isoelectric focusing and immunoblotting. A constant low level of endoxylanase expression was observed in glucose medium. A high level of xyla...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13066.x

    authors: Gomez de Segura B,Durand R,Fèvre M

    更新日期:1998-07-01 00:00:00

  • Comparative analysis of gene expression in Streptococcus pneumoniae and Lactococcus lactis.

    abstract::The pFL10 plasmid vector for translational fusions was constructed. pFL10 is based in the promiscuous pLS1 replicon and contains the pC194 cat gene deprived of its transcriptional promoter and Shine-Dalgarno (SD) sequence. Three promoters (Pcit, PpolA and PtetL) from Gram-positive bacteria, inserted in pFL10, were tes...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07182.x

    authors: López de Felipe F,Corrales MA,López P

    更新日期:1994-10-01 00:00:00

  • Distribution of the IS elements ISS1 and IS904 in lactococci.

    abstract::A broad distribution of the lactococcal IS elements ISS1 [1] and IS904 [2] in several lactococcal plasmids and chromosomal DNA was observed. Hybridization of the ISS1 and IS904 oligonucleotide gene probes with DNA of lactococcal phages showed that none of these tested bacteriophages contained one of the IS elements. O...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1991.tb04681.x

    authors: Schäfer A,Jahns A,Geis A,Teuber M

    更新日期:1991-05-15 00:00:00

  • Extracellular proteolytic activation of bacteriolytic peptidoglycan hydrolases of Staphylococcus simulans biovar staphylolyticus.

    abstract::Staphylococcus simulans biovar staphylolyticus secreted two bacteriolytic peptidoglycan hydrolases as proproteins that were activated as they were processed by an extracellular sulphydryl protease. This processing resulted in the production of multiple molecular-mass forms of each enzyme. Cells from early exponential ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06321.x

    authors: Neumann VC,Heath HE,LeBlanc PA,Sloan GL

    更新日期:1993-06-15 00:00:00