Increased expression and hotspot mutations of the multidrug efflux transporter, CDR1 in azole-resistant Candida albicans isolates from vaginitis patients.

abstract：:micF RNA, whose sequence is highly complementary to a 5'-portion of ompF mRNA, has been implicated in the osmoregulation and thermoregulation of the ompF porin gene in Escherichia coli. To define and characterize cis-acting regulatory regions upstream of the micF promoter, a series of deletions of the micF promoter fu...

journal_title：FEMS microbiology letters

authors： Takayanagi K,Maeda S,Mizuno T

更新日期：1991-09-15 00:00:00

abstract：:Probing protein extracts from exponentially growing and stationary phase cultures of Mycobacterium bovis BCG with anti-phospho amino acid antibodies revealed a 31-kDa anti-phospho threonine antibody-reactive protein specific to growing culture. The corresponding protein was purified via two-dimensional gel electrophor...

journal_title：FEMS microbiology letters

authors： Sinha I,Boon C,Dick T

更新日期：2003-10-10 00:00:00

abstract：:The small heat shock protein (smHsp) Lo18 from lactic acid bacteria Oenococcus oeni reduces in vitro thermal aggregation of proteins and modulates the membrane fluidity of native liposomes. An absence of information relating to the way in which the smHsp demonstrates a stabilizing effect for both proteins and membrane...

journal_title：FEMS microbiology letters

authors： Weidmann S,Rieu A,Rega M,Coucheney F,Guzzo J

更新日期：2010-08-01 00:00:00

abstract：:The purpose of this study was to generate highly specific serological reagents for the quantitative identification of Actinomyces naeslundii in clinical samples, in particular dental plaque. Balb/c mice were immunized with pasteurized human A. naeslundii strains representing different genospecies and serotypes. Ten hy...

journal_title：FEMS microbiology letters

authors： Thurnheer T,Guggenheim B,Gmür R

更新日期：1997-05-15 00:00:00

abstract：:The phylogenetic position of an acidophilic chemo-organotrophic menaquinone-containing bacterium, Acidobacterium capsulatum, was studied on the basis of 16S rRNA gene sequence information. A. capsulatum showed the highest level of sequence similarity to Heliobacterium chlorum, a member of the Gram-positive group, yet ...

journal_title：FEMS microbiology letters

authors： Hiraishi A,Kishimoto N,Kosako Y,Wakao N,Tano T

更新日期：1995-10-01 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:A CO-reactive hemoprotein was purified from the mitochondrial membrane fraction of Tetrahymena pyriformis. It showed absorption peaks at 615 and 455 nm in the reduced form and an alpha peak at 565 nm in the pyridine ferrohemochrome spectrum. Although the spectral properties were apparently similar to those of 'cytochr...

journal_title：FEMS microbiology letters

authors： Inokuchi A,Fukumori Y

更新日期：1993-08-15 00:00:00

abstract：:Brucella melitensis possesses an operon with two components: the response regulator OtpR and a putative cAMP-dependent protein kinase regulatory subunit encoded by the BMEI0067 gene. In the previous study, the function of OtpR has been studied, while little is known about the function of the BMEI0067 gene. Using a bio...

journal_title：FEMS microbiology letters

authors： Liu WJ,Dong H,Peng XW,Wu QM

更新日期：2014-07-01 00:00:00

abstract：:The polymerase chain reaction (PCR)-based procedures of randomly amplified polymorphic DNA (RAPD) and repetitive element (RE)-based PCR were used to amplify total DNA prepared from each of 62 clinical Serratia marcescens isolates. Three different random primers, designated 1060, 1254 and 1283, were used individually i...

journal_title：FEMS microbiology letters

authors： Patton TG,Katz S,Sobieski RJ,Crupper SS

更新日期：2001-01-01 00:00:00

abstract：:The CotSA protein, encoded by cotSA (ytxN) of Bacillus subtilis, was detected from the cells at 5 h after the onset of sporulation (T5) and in the spore coat of wild-type cells, but not in cotE, cotS, gerE, or cotSA mutant spores. CotSA was also detected in the sporangium at T5 to T7 but not in the sporangium at T18 o...

journal_title：FEMS microbiology letters

authors： Takamatsu H,Kodama T,Watabe K

更新日期：1999-05-01 00:00:00

abstract：:Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...

journal_title：FEMS microbiology letters

authors： Liu X,Feng Z,Harris NB,Cirillo JD,Bercovier H,Barletta RG

更新日期：2001-08-21 00:00:00

abstract：:The American Society for Microbiology's curricular guidelines for Introductory Microbiology highlighted key laboratory skills in the isolation, visualization and identification of microorganisms as core learning objectives in the discipline. Since the publication of these guidelines in 2012, there has been a paucity o...

journal_title：FEMS microbiology letters

authors： Wang JTH,Huston WM,Johanesen P,Lloyd M,Waller KL

更新日期：2018-10-01 00:00:00

abstract：:Pseudomonas aeruginosa is a Gram-negative pathogen and the major cause of mortality in patients with cystic fibrosis. The mechanisms that P. aeruginosa strains use to regulate intracellular zinc have an effect on infection, antibiotic resistance and the propensity to form biofilms. However, zinc homeostasis in P. aeru...

journal_title：FEMS microbiology letters

authors： Davies CB,Harrison MD,Huygens F

更新日期：2017-09-01 00:00:00

abstract：:A complementary DNA of the Aspergillus nidulans chsB gene encoding chitin synthase, an essential gene for hyphal growth, was obtained by RT-PCR and expressed in Saccharomyces cerevisiae by using the GAL1 promoter in a multicopy plasmid. The biochemical characteristics of chitin synthase B (ChsB) expressed in S. cerevi...

journal_title：FEMS microbiology letters

authors： Tatsuno K,Yamada-Okabe H,Takagi M,Arisawa M,Sudoh M

更新日期：1997-04-15 00:00:00

abstract：:We examined the effect of the pyridoxal 5'-phosphate (PLP) cofactor on the activity and stability of the psychrophilic alanine racemase, having a high catalytic activity at low temperature, from Bacillus psychrosaccharolyticus at high temperatures. The decrease in the enzyme activity at incubation temperatures over 40...

journal_title：FEMS microbiology letters

authors： Okubo Y,Yokoigawa K,Esaki N,Soda K,Misono H

更新日期：2000-11-15 00:00:00

abstract：:The nucleotide sequence of the gene specifying the ethidium efflux system of Escherichia coli has been determined. The translated open reading frame has identified a membrane-bound polypeptide of 110 amino acids (11,960 Da) which shares 42% identity with a staphylococcal protein specifying resistance to ethidium. ...

journal_title：FEMS microbiology letters

authors： Purewal AS

更新日期：1991-08-01 00:00:00

abstract：:Weissella cibaria isolated from human saliva produces a soluble glucan that predominantly has alpha-1,6-glucosidic type linkages. Using degenerated primers that were selected based on the amino acid sequences of conserved regions from known glucansucrases, a single 2.7-kb fragment was isolated. In subsequent steps, a ...

journal_title：FEMS microbiology letters

authors： Kang HK,Oh JS,Kim D

更新日期：2009-03-01 00:00:00

abstract：:Aceticlastic methanogens metabolize acetate to methane and carbon dioxide. The central metabolism and the electron transport chains of these organisms have already been investigated. However, no particular attention has been paid to the mechanism by which acetate enters the archaeal cell. In our study we investigated ...

journal_title：FEMS microbiology letters

authors： Welte C,Kröninger L,Deppenmeier U

更新日期：2014-10-01 00:00:00

abstract：:SPC(3) is a multiple antigen peptide derived from the V(3) loop of human immunodeficiency virus (HIV) envelope (Env). It exerts a potent anti-HIV activity whereas it alters neither Env expression nor binding to CD(4). Here, SPC(3) binding characteristics, its subsequent intracellular fate and the fact that it inhibite...

journal_title：FEMS microbiology letters

authors： Barbouche R,Papandréou MJ,Miquelis R,Guieu R,Fenouillet E

更新日期：2000-02-15 00:00:00

abstract：:In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

journal_title：FEMS microbiology letters

authors： Wolters M,Borst A,Pfeiffer F,Soppa J

更新日期：2019-11-01 00:00:00

abstract：:Bacillus subtilis strains BS49 and BS34A, both derived from a common ancestor, carry one or more copies of Tn916, an extremely common mobile genetic element capable of transfer to and from a broad range of microorganisms. Here, we report the complete genome sequence of BS49 and the draft genome sequence of BS34A, whic...

journal_title：FEMS microbiology letters

authors： Browne HP,Anvar SY,Frank J,Lawley TD,Roberts AP,Smits WK

更新日期：2015-01-01 00:00:00

abstract：:The cyanobacterium Synechocystis 6803 was tagged by chromosomal integration of the firefly luciferase gene, luc, resulting in the modified strain Synechocystis 6803-luc. The tagged cells were monitored in Baltic Sea microcosms both by detection of the luc gene by PCR amplification and by measurement of luc gene expres...

journal_title：FEMS microbiology letters

authors： Möller A,Norrby AM,Gustafsson K,Jansson J

更新日期：1995-06-01 00:00:00

abstract：:The multiple antibiotic resistance operon (marRAB) is a member of the multidrug resistance (mdr) systems. Similar to other mdr systems, this operon when induced encodes resistance to structurally and functionally unrelated antibiotics. marRAB has been shown to be conserved in the family Enterobacteriaceae, but within ...

journal_title：FEMS microbiology letters

authors： Kunonga NI,Sobieski RJ,Crupper SS

更新日期：2000-06-15 00:00:00

abstract：:This study reports the production of astaxanthin in the photosynthetic bacterium Rhodovulum sulfidophilum, which has adequate precursor pools and storage capabilities for heterologous carotenoid production. Chemical mutagenesis was carried out using ethylmethane sulfonate to produce mutants with a modified carotenoid ...

journal_title：FEMS microbiology letters

authors： Mukoyama D,Takeyama H,Kondo Y,Matsunaga T

更新日期：2006-12-01 00:00:00

abstract：:Bjerkandera adusta produces many chlorometabolites including chlorinated anisyl metabolites (CAMs) and 1-arylpropane-1,2-diols (1, 2, 3, 4) as idiophasic metabolic products of L-phenylalanine. These diols are stereoselectively biosynthesized from a C7-unit (benzylic, from L-phenylalanine) and a C2-unit, of unknown ori...

journal_title：FEMS microbiology letters

authors： Silk PJ,Macaulay JB

更新日期：2003-11-07 00:00:00

abstract：:Pneumocystis is a parasitic fungus causing pneumonia in immunosuppressed mammals and S-adenosylmethionine a key intermediary metabolite for all cells. Other than a species of Rickettsia bacteria and an aberrant strain of the protozoan Amoeba proteus, Pneumocystis is the only cell known unable to synthesize AdoMet; it ...

journal_title：FEMS microbiology letters

authors： Merali S,Clarkson AB Jr

更新日期：2004-08-15 00:00:00

abstract：:We present a method to directly characterize the yeast diversity present in wine fermentations by employing denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 26S ribosomal RNA (rRNA) genes. PCR-DGGE of a portion of the 26S rRNA gene was shown to distinguish most yeast genera a...

journal_title：FEMS microbiology letters

authors： Cocolin L,Bisson LF,Mills DA

更新日期：2000-08-01 00:00:00

abstract：:The number and incidence of Collinsella aerofaciens in the human intestine are the highest among Gram-positive non-spore-forming bacilli. Identification of this species is very difficult and requires considerable time. A PCR-based identification system using C. aerofaciens-specific primers is described. Using this PCR...

journal_title：FEMS microbiology letters

authors： Kageyama A,Sakamoto M,Benno Y

更新日期：2000-02-01 00:00:00

abstract：:Isolated membranes of the extreme haloalkaliphilic archaeon Natronococcus occultus were able to hydrolyze ATP via an ATPase, which required the presence of Mg(2+), high concentrations of NaCl, and a pH value of 9. The native molecular mass of the purified ATPase was 130 kDa and was composed of 74- and 61-kDa subunits....

journal_title：FEMS microbiology letters

authors： Eddy ML,Jablonski PE

更新日期：2000-08-15 00:00:00

abstract：:The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...

journal_title：FEMS microbiology letters

authors： Esteves MG,Gonzales-Perdomo M,Alviano CS,Angluster J,Goldenberg S

更新日期：1989-05-01 00:00:00