The phylogenetic position of Hydrogenobacter acidophilus based on 16S rRNA sequence analysis.

abstract：:The identification of virulence factors in Rhodococcus equi has been severely hampered by the lack of a method for in vivo random insertion mutagenesis. This study reports the use of transposomes to generate random insertions of a gene conferring kanamycin resistance into the genome of R. equi ATCC 33701. Southern hyb...

journal_title：FEMS microbiology letters

authors： Mangan MW,Meijer WG

更新日期：2001-12-18 00:00:00

abstract：:A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculat...

journal_title：FEMS microbiology letters

authors： Akiba M,Sameshima T,Nakazawa M

更新日期：2000-03-01 00:00:00

abstract：:Studies on cultured cells and in infection models have shown that cell density-dependent quorum-sensing (QS) controls many of the known virulence factors of Pseudomonas aeruginosa. However, it is less clear what role QS plays in chronic human lung infections associated with cystic fibrosis (CF). The involvement of QS ...

journal_title：FEMS microbiology letters

authors： Winstanley C,Fothergill JL

更新日期：2009-01-01 00:00:00

abstract：:The physiology of the response in the methanotrophic bacterium Methylococcus capsulatus Bath towards thermal and solvent stress was studied. A systematic investigation of the toxic effects of organic compounds (chlorinated phenols and alkanols) on the growth of this bacterium was carried out. The sensitivity to the te...

journal_title：FEMS microbiology letters

authors： Löffler C,Eberlein C,Mäusezahl I,Kappelmeyer U,Heipieper HJ

更新日期：2010-07-01 00:00:00

abstract：:Staphylococcus aureus represents the most prevalent cause of food-borne intoxications worldwide. While being repressed by competing bacteria in most matrices, this pathogen exhibits crucial competitive advantages during growth at high salt concentrations or low pH, conditions frequently encountered in food production ...

journal_title：FEMS microbiology letters

authors： Sihto HM,Tasara T,Stephan R,Johler S

更新日期：2014-07-01 00:00:00

abstract：:In the purple sulphur bacterium Allochromatium vinosum, the prosthetic group of dissimilatory sulphite reductase (DsrAB) was identified as siroamide, an amidated form of the classical sirohaem. The genes dsrAB are the first two of a large cluster of genes necessary for the oxidation of sulphur globules stored intracel...

journal_title：FEMS microbiology letters

authors： Lübbe YJ,Youn HS,Timkovich R,Dahl C

更新日期：2006-08-01 00:00:00

abstract：:Aspergillus niger represents a promising host for the expression of recombinant proteins, but only a few expression systems are available for this organism. In this study, the inducible catalase promoter (PcatR) from A. niger was characterized. For this, constructs were developed and checked for the expression of the ...

journal_title：FEMS microbiology letters

authors： Sharma R,Katoch M,Govindappa N,Srivastava PS,Sastry KN,Qazi GN

更新日期：2012-02-01 00:00:00

abstract：:A cold shock domain (CSD)-containing protein, CspD, of molecular mass ~7.28 kDa in a psychrotolerant Antarctic Janthinobacterium sp. Ant5-2 (ATCC BAA-2154) exhibited constitutive expression at 37, 22, 15, 4 and -1°C. The cspD gene encoding the CspD protein of Ant5-2 was cloned, sequenced and analyzed. The deduced prot...

journal_title：FEMS microbiology letters

authors： Mojib N,Andersen DT,Bej AK

更新日期：2011-06-01 00:00:00

abstract：:Fluorescence in situ hybridisation (FISH) has become one of the major techniques in environmental microbiology. The original version of this technique often suffered from limited sensitivity due to low target copy number or target inaccessibility. In recent years there have been several developments to amend this prob...

journal_title：FEMS microbiology letters

authors： Zwirglmaier K

更新日期：2005-05-15 00:00:00

abstract：:The gene encoding Aquifex aeolicus (Aae) DNA polymerase was expressed under the control of the trp promoter on a high-copy plasmid, pTRPNS, in Escherichia coli. The expressed enzyme was purified 11-fold with a 13.8% yield and a specific activity of 2268.3 U mg(-1). The optimum pH of the enzyme was 6.8-7.2. The optimal...

journal_title：FEMS microbiology letters

authors： Chang JR,Choi JJ,Kim HK,Kwon ST

更新日期：2001-07-10 00:00:00

abstract：:The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated...

journal_title：FEMS microbiology letters

authors： Kurth JM,Schuster A,Seel W,Herresthal S,Simon J,Dahl C

更新日期：2017-02-01 00:00:00

abstract：:The bft gene encoding Bacteroides fragilis toxin (BFT) has been devided into two subtypes, bft-1 and bft-2. We found a novel subtype by sequencing a segment of the bft gene from 64 enterotoxigenic B. fragilis (ETBF) strains isolated in Japan. The 1548-bp nucleotide sequences of the new bft, the bft-1, and bft-2 genes ...

journal_title：FEMS microbiology letters

authors： Kato N,Liu CX,Kato H,Watanabe K,Tanaka Y,Yamamoto T,Suzuki K,Ueno K

更新日期：2000-01-01 00:00:00

abstract：:The influence of nutritional and physical stress on sporulation, conidial germination and vegetative biomass of Ophiocordyceps sinensis, one of the most important medicinal fungi in China and now globally, was evaluated using a two-stage culture method. All the treatments, except nutrient deprivation, enhanced conidia...

journal_title：FEMS microbiology letters

authors： Ren SY,Yao YJ

更新日期：2013-09-01 00:00:00

abstract：:Rhodococcus rhodochrous NCIMB13064 can dehalogenate and utilise a number of halogenated aliphatic compounds as sole carbon and energy source. Mutants of NCIMB13064 can be easily isolated with an enlarged range of 1-chloroalkane utilising ability. Dehalogenation of 1-chlorononane, 1-chlorodecane and short-chain 1-chlor...

journal_title：FEMS microbiology letters

authors： Kulakova AN,Reid KA,Larkin MJ,Allen CC,Kulakov LA

更新日期：1996-12-01 00:00:00

abstract：:Researchers studying both cellular and viral disease agents in the laboratory have become infected since the early days of microbiology 150 years ago. However, in the early 21st century, new concerns about bioweapons being used to generate terror and also with a series of newly emerging or newly understood disease-cau...

journal_title：FEMS microbiology letters

authors： Silver S

更新日期：2015-01-01 00:00:00

abstract：:During programs for the selection of enological yeasts, several hundred natural isolates are usually screened. The scope of these operations is to isolate strains possessing good fermentative properties without necessarily arriving at a precise species designation: in other words, to detect strains belonging to the Sa...

journal_title：FEMS microbiology letters

authors： Nardi T,Carlot M,De Bortoli E,Corich V,Giacomini A

更新日期：2006-11-01 00:00:00

abstract：:Linkage of rumen microbial structure to host phenotypical traits may enhance the understanding of host-microbial interactions in livestock species. This study used culture-independent PCR-denaturing gradient gel electrophoresis (PCR-DGGE) to investigate the microbial profiles in the rumen of cattle differing in feed e...

journal_title：FEMS microbiology letters

authors： Guan LL,Nkrumah JD,Basarab JA,Moore SS

更新日期：2008-11-01 00:00:00

abstract：:We present evidence that the anthrax toxin lethal factor binds multiple zinc atoms. Results from atomic adsorption spectroscopy indicate that lethal factor contains approximately three zinc atoms per toxin molecule. Lethal factor treated with EDTA and o-phenanthroline contained a similar number of zinc atoms, indicati...

journal_title：FEMS microbiology letters

authors： Kochi SK,Schiavo G,Mock M,Montecucco C

更新日期：1994-12-15 00:00:00

abstract：:A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 other bacterial species. A detection limit of 1 cell equivalent wa...

journal_title：FEMS microbiology letters

authors： O'Grady J,Lacey K,Glynn B,Smith TJ,Barry T,Maher M

更新日期：2009-12-01 00:00:00

abstract：:A previous study [DeVault et al. (1991) Mol. Microbiol. 5, 2503-2509] suggested that growth of Pseudomonas aeruginosa in glucose-containing medium represses algD gene transcription. In this study, growth of P. aeruginosa in rich medium containing glucose or gluconate increased alginate production and algD transcriptio...

journal_title：FEMS microbiology letters

authors： Ma JF,Phibbs PV,Hassett DJ

更新日期：1997-03-15 00:00:00

abstract：:Plasmodium falciparum sirtuin, PfSir2, contains histone deacetylase (HDAC) activity that may be central to the regulation of virulence gene expression in the parasites. Although a few reports have been published recently regarding in vitro and in vivo function of PfSir2, expression of the endogenous protein (c. 30 kDa...

journal_title：FEMS microbiology letters

authors： Prusty D,Mehra P,Srivastava S,Shivange AV,Gupta A,Roy N,Dhar SK

更新日期：2008-05-01 00:00:00

abstract：:A plasmid-based food-grade vector system was developed for Lactococcus lactis by exploiting the genes for lactose metabolism. L. lactis MG5267 is a plasmid-free strain containing the entire lactose operon as a chromosomal insertion. The lacF gene was deleted from this strain by a double cross-over homologous recombina...

journal_title：FEMS microbiology letters

authors： MacCormick CA,Griffin HG,Gasson MJ

更新日期：1995-03-15 00:00:00

abstract：:Bordetella pertussis is the causative agent of whooping cough. Traditional vaccines against this disease are inherently reactogenic, thus research is currently focussed on the production of less reactive, acellular vaccines. Expression of candidate antigens for these vaccines in Escherichia coli would be preferable, h...

journal_title：FEMS microbiology letters

authors： Smith AM,Yan H,Groves N,Dalla Pozza T,Walker MJ

更新日期：2000-10-15 00:00:00

abstract：:The porin-associated protein of Rhodospirillum rubrum FR1 was found to contain a peptidoglycan binding motif. A partial fragment of 179 amino acids, obtained by cleavage of PAP with trypsin, Asp-N protease, and CNBr, was sequenced. Substantial sequence homology was found of the C-terminal part (residues 126-179) of po...

journal_title：FEMS microbiology letters

authors： Neumann U,Schiltz E,Stahl B,Hillenkamp F,Weckesser J

更新日期：1996-04-15 00:00:00

abstract：:Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y. pestis genome databases revealed the presence of n...

journal_title：FEMS microbiology letters

authors： Bobrov AG,Perry RD

更新日期：2006-02-01 00:00:00

abstract：:Bacterial specific primers were used to amplify 23S rRNA genes from a representative strain from each of the 23 serogroups of the pathogenic Leptospira interrogans and 8 strains from 6 serogroups of the non-pathogenic Leptospira biflexa. Only regions of extreme variability, which had been identified on the basis of ho...

journal_title：FEMS microbiology letters

authors： Woo TH,Smythe LD,Symonds ML,Norris MA,Dohnt MF,Patel BK

更新日期：1997-05-01 00:00:00

abstract：:Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two grou...

journal_title：FEMS microbiology letters

authors： Yamamura H,Hanawa K,Kusunoki M,Nakamura K,Nakagawa Y,Ishida Y,Hamada M,Otoguro M,Tamura T,Fujita N,Hayakawa M

更新日期：2011-11-01 00:00:00

abstract：:In several bacterial species that show natural transformation, dprA has been described as a competence gene. The DprA protein has been suggested to be involved in the protection of incoming DNA. However, members of the dprA gene family (also called smf) can be detected in virtually all bacterial species, which suggest...

journal_title：FEMS microbiology letters

authors： Smeets LC,Becker SC,Barcak GJ,Vandenbroucke-Grauls CM,Bitter W,Goosen N

更新日期：2006-10-01 00:00:00

abstract：:Mesophilic Aeromonas hydrophila from serotypes O:11 and O:34 grown in a glucose-rich medium produce a capsule that can be seen under light and electron microscopy. The purified capsular polysaccharide has a composition qualitatively similar for strains O:11 and O:34, but quantitatively different. The capsular polysacc...

journal_title：FEMS microbiology letters

authors： Martínez MJ,Simon-Pujol D,Congregado F,Merino S,Rubires X,Tomás JM

更新日期：1995-04-15 00:00:00

abstract：:A bacterial isolate, tentatively identified as Pseudomonas sp. strain TR3, was found to utilize the diaryl ester phenylbenzoate as sole source of carbon and energy. This strain has the ability to productively degrade phenylbenzoate and some substituted derivatives by a catabolic sequence which was characterized bioche...

journal_title：FEMS microbiology letters

authors： Reich T,Schmidt S,Fortnagel P

更新日期：1999-07-15 00:00:00