Abstract:
:Bacterial specific primers were used to amplify 23S rRNA genes from a representative strain from each of the 23 serogroups of the pathogenic Leptospira interrogans and 8 strains from 6 serogroups of the non-pathogenic Leptospira biflexa. Only regions of extreme variability, which had been identified on the basis of homology-based search of all the 23S rRNA sequences available in GenBank database, were sequenced from the amplified products. PCR primers that had the potential to distinguish L. interrogans from L. biflexa species were designed from the derived sequences and a sensitive PCR protocol developed. The PCR method enabled the differentiation of the 59 strains of the 23 serogroups of L. interrogans from the 8 strains of 6 serogroups of L. biflexa. Further investigation by 16S rDNA sequencing of two strains of L. interrogans, which gave unexpected PCR results, provided evidence that they had been misclassified and hence we propose to reassign them to L. biflexa.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Woo TH,Smythe LD,Symonds ML,Norris MA,Dohnt MF,Patel BKdoi
10.1111/j.1574-6968.1997.tb10343.xsubject
Has Abstractpub_date
1997-05-01 00:00:00pages
9-18issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(97)00066-9journal_volume
150pub_type
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