The isolation and characterisation of a Saccharomyces cerevisiae gene (LIP2) involved in the attachment of lipoic acid groups to mitochondrial enzymes.

Abstract:

:Lipoic acid is an essential cofactor for a variety of mitochondrial enzymes. We have characterised a gene from Saccharomyces cerevisiae which appears to encode a protein involved in the attachment of lipoic acid groups to the pyruvate dehydrogenase and glycine decarboxylase complexes. The predicted protein product of this gene has significant identity to the lipoyl ligase B of both Escherichia coli and Kluyveromyces lactis. A strain harbouring a null allele of this S. cerevisiae gene is respiratory deficient due to inactive pyruvate dehydrogenase, and is unable to utilise glycine as a sole nitrogen source.

journal_name

FEMS Microbiol Lett

authors

Marvin ME,Williams PH,Cashmore AM

doi

10.1111/j.1574-6968.2001.tb10663.x

keywords:

subject

Has Abstract

pub_date

2001-05-15 00:00:00

pages

131-6

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(01)00170-7

journal_volume

199

pub_type

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