Abstract:
:Lipoic acid is an essential cofactor for a variety of mitochondrial enzymes. We have characterised a gene from Saccharomyces cerevisiae which appears to encode a protein involved in the attachment of lipoic acid groups to the pyruvate dehydrogenase and glycine decarboxylase complexes. The predicted protein product of this gene has significant identity to the lipoyl ligase B of both Escherichia coli and Kluyveromyces lactis. A strain harbouring a null allele of this S. cerevisiae gene is respiratory deficient due to inactive pyruvate dehydrogenase, and is unable to utilise glycine as a sole nitrogen source.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Marvin ME,Williams PH,Cashmore AMdoi
10.1111/j.1574-6968.2001.tb10663.xkeywords:
subject
Has Abstractpub_date
2001-05-15 00:00:00pages
131-6issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(01)00170-7journal_volume
199pub_type
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