Abstract:
:The enteric conjugative transposon-like IncJ elements R391, R392, R705, R706 and pMERPH, all demonstrated increased conjugative transfer upon UV irradiation. The transfer frequency increased on average from its basal rate of 10(-5) to 10(-3) per recipient, upon pre-exposure to UV irradiation. However, the transfer frequency of R997, which was higher than the other IncJ elements at 10(-3) per donor, showed a smaller increase. This effect was shown to be recA+ dependent in all cases. Using PCR primers directed outwards from the ends of the integrated R391 element it was observed that a circular intermediate of the element forms within the host, which has been proposed to be a transfer intermediate. Using real-time PCR, it was determined that the amount of the circular intermediate produced increased substantially upon UV irradiation.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
McGrath BM,O'Halloran JA,Pembroke JTdoi
10.1016/j.femsle.2005.01.013keywords:
subject
Has Abstractpub_date
2005-02-15 00:00:00pages
461-5issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(05)00015-7journal_volume
243pub_type
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