A molecular approach for early monitoring of decay basidiomycetes in wood chips.

abstract：:Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

journal_title：FEMS microbiology letters

authors： Kouzuma A,Hashimoto K,Watanabe K

更新日期：2012-01-01 00:00:00

abstract：:Small plasmids were found in two clinical isolates of Streptococcus pneumoniae from Spain (strains 671 and 678) and in one strain (SpR) isolated in Germany. All three strains contained one plasmid (2.75 to 3.1 kb) which is related to the only previously described pneumococcal plasmid, pDP1. Strains 678 and SpR carried...

journal_title：FEMS microbiology letters

authors： Sibold C,Markiewicz Z,Latorre C,Hakenbeck R

更新日期：1991-01-01 00:00:00

abstract：:An exo-polygalacturonase (EC 3.2.1.15) was purified to apparent homogeneity from cultures of Fusarium oxysporum f.sp. lycopersici on synthetic medium supplemented with citrus pectin, using preparative isoelectric focusing. The enzyme, denominated PG2, had an apparent M(r) of 74000 Da upon SDS-PAGE. The pI of the main ...

journal_title：FEMS microbiology letters

authors： Di Pietro A,Roncero MI

更新日期：1996-12-01 00:00:00

abstract：:To improve expression efficiency of the leuB gene of an extreme thermophile. Thermus thermophilus, in Escherichia coli, the gene was placed under a potent promoter, tac. However, the expression was hardly improved, despite increased transcription. The expression under tac promoter was significantly improved by introdu...

journal_title：FEMS microbiology letters

authors： Ishida M,Oshima T

更新日期：1996-01-01 00:00:00

abstract：:The transport of radioactively labelled uracil into submerged mycelium of T. viride was measured by means of a membrane filtration technique. It was found to be time-dependent (up to 90 min) and concentration-dependent (up to 8 mmol l-1). Its concentration dependence was biphasic and consisted from the saturatable par...

journal_title：FEMS microbiology letters

authors： Lakatos B,Simkovic M,Betina V,Varecka L

更新日期：1999-02-15 00:00:00

abstract：:Brucella abortus B19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. B19 differs from other B. abortus strains in its sensitivity to erythritol. We took advantage of a previously obtained erythritol sensitive Tn5 insertion mutant of B. abortu...

journal_title：FEMS microbiology letters

authors： Sangari FJ,García-Lobo JM,Agüero J

更新日期：1994-09-01 00:00:00

abstract：:Tn4560 (8.6 kb) was derived from Tn4556, a Tn3-like element from Streptomyces fradiae. It contains a viomycin resistance gene that has not been used previously for selection in mycobacteria. Tn4560, cloned in a Streptomyces plasmid, was introduced by electroporation into Mycobacterium smegmatis mc(2)155. Tn4560 transp...

journal_title：FEMS microbiology letters

authors： Bhatt A,Stewart GR,Kieser T

更新日期：2002-01-10 00:00:00

abstract：:A 2918 bp sequence coding for the orotidine-5'-monophosphate decarboxylase enzyme (OMPD) was isolated from the genome of Myrothecium gramineum. This sequence was analysed and, remarkably, it is the first OMPD gene of a Sordariomycete that has an intron. The gene codes for an enzyme of 282 amino acids. The nucleotide s...

journal_title：FEMS microbiology letters

authors： De Maeseneire SL,De Groeve MR,Dauvrin T,De Mey M,Soetaert W,Vandamme EJ

更新日期：2006-08-01 00:00:00

abstract：:Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the vari...

journal_title：FEMS microbiology letters

authors： Fekete C,Giczey G,Papp I,Szabó L,Hornok L

更新日期：1995-09-15 00:00:00

abstract：:Food-borne diseases are a threat to human health and can cause severe economic losses. Nowadays, in a growing and increasingly interconnected world, food-borne diseases need to be dealt with in a global manner. In order to tackle this issue, it is essential to consider all possible entry routes of human pathogens into...

journal_title：FEMS microbiology letters

authors： Schierstaedt J,Grosch R,Schikora A

更新日期：2019-12-01 00:00:00

abstract：:The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

journal_title：FEMS microbiology letters

authors： Argüelles JC,Argüelles-Prieto R

更新日期：2019-06-01 00:00:00

abstract：:Pseudomonas aeruginosa are known to have a wide physiological potential allowing them to constantly populate diverse environments leading to severe infections of humans such as septicemia, leg ulcers, and burn wounds. We set out to probe physiological characteristics of P. aeruginosa isolates from diabetic leg ulcers ...

journal_title：FEMS microbiology letters

authors： Buivydas A,Pasanen T,Senčilo A,Daugelavičius R,Vaara M,Bamford DH

更新日期：2013-06-01 00:00:00

abstract：:The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF pr...

journal_title：FEMS microbiology letters

authors： Grassl G,Bufe B,Müller B,Rösel M,Kleiner D

更新日期：1999-10-01 00:00:00

abstract：:Bacteria have the exquisite ability to maintain a precise diameter, cell length, and shape. The dimensions of bacteria size and shape are a classical metric in the distinction of bacterial species. Much of what we know about the particular morphology of any given species is the result of investigations of planktonic c...

journal_title：FEMS microbiology letters

authors： Justice SS,Harrison A,Becknell B,Mason KM

更新日期：2014-11-01 00:00:00

abstract：:We recently identified the phaG(Pp) gene encoding (R)-3-hydroxydecanoyl-ACP:CoA transacylase in Pseudomonas putida, which directly links the fatty acid de novo biosynthesis and polyhydroxyalkanoate (PHA) biosynthesis. An open reading frame (ORF) of which the deduced amino acid sequence shared about 57% identity with P...

journal_title：FEMS microbiology letters

authors： Hoffmann N,Steinbüchel A,Rehm BH

更新日期：2000-03-15 00:00:00

abstract：:The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

journal_title：FEMS microbiology letters

authors： Alon RN,Gutnick DL

更新日期：1993-09-15 00:00:00

abstract：:To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulm...

journal_title：FEMS microbiology letters

authors： Moran AJ,Treit JD,Whitney JL,Abomoelak B,Houghton R,Skeiky YA,Sampaio DP,Badaró R,Nano FE

更新日期：2001-04-20 00:00:00

abstract：:In a previous study, we isolated the spaA gene encoding the surface protective antigen A, SpaA, of Erysipelothrix rhusiopathiae, and found that the N-terminal region of SpaA was responsible for protective immunity against erysipelas and that the C-terminal region contained eight repeat units consisting of 20 amino aci...

journal_title：FEMS microbiology letters

authors： Makino SI,Yamamoto K,Asakura H,Shirahata T

更新日期：2000-05-15 00:00:00

abstract：:There are arguments against the conclusion drawn by Haselwandter and Ebner that fungal spores have survived for some 5300 years on hay padding in the leather boots of a frozen body discovered in the Austrian Alps. According to cryobiological experience, long-term survival of fungal spores is very unlikely at temperatu...

journal_title：FEMS microbiology letters

authors： Gams W,Stalpers JA

更新日期：1994-07-01 00:00:00

abstract：:We have identified, cloned and characterized a formerly unknown protein from Streptomyces lividans spores. The deduced protein belongs to a novel member of the metallophosphatase superfamily and contains a phosphatase domain and predicted binding sites for divalent ions. Very close relatives are encoded in the genomic...

journal_title：FEMS microbiology letters

authors： Lamp J,Weber M,Cingöz G,Ortiz de Orué Lucana D,Schrempf H

更新日期：2013-05-01 00:00:00

abstract：:Clostridium thermocellum, a Gram-positive, thermophilic anaerobe produces a highly active cellulase system. This system, termed the cellulosome, is a complex composed of at least 14-18 different types of components organized around a large, cellulose-binding protein. Combining recombinant DNA technology and protein bi...

journal_title：FEMS microbiology letters

authors： Béguin P,Millet J,Aubert JP

更新日期：1992-12-15 00:00:00

abstract：:The enzymatic activity of salivary amylase bound to the surface of several species of oral streptococci was determined by the production of acid from starch and by the degradation of maltotetraose to glucose in a coupled, spectrophotometric assay. Most strains able to bind amylase exhibited functional enzyme on their ...

journal_title：FEMS microbiology letters

authors： Douglas CW,Heath J,Gwynn JP

更新日期：1992-04-15 00:00:00

abstract：:Macrolide is inactivated with ATP plus crude extract of Escherichia coli producing macrolide 2'-phosphotransferase (MPH(2')), but not by living cells. Therefore, a convenient method for detection of MPH(2') using intact cells is needed. In this report, we determine that the modified lysozyme-DNase-RNase (LDR) method (...

journal_title：FEMS microbiology letters

authors： Taniguchi K,Nakamura A,Tsurubuchi K,O'Hara K,Sawai T

更新日期：1998-10-15 00:00:00

abstract：:Two type B15 P1.16 strains of Neisseria meningitidis were examined by immunogold electron microscopy for accessibility of two outer membrane protein (OMPs) to monoclonal antibodies. Both strains exhibited cell-to-cell variation of one epitope of the Class 3 OMP (P3.15) and one of the Class 1 OMPs (P1.16) when grown in...

journal_title：FEMS microbiology letters

authors： Wall RA,Davies HA,Borriello SP

更新日期：1989-11-01 00:00:00

abstract：:Salmonella is a leading cause of waterborne diseases. Salmonella can survive for a long time in aquatic environments, and its persistence in the environment is of great concern to public health. Nonetheless, the presence and diversity of Salmonella in the aquatic environments in most areas remain relatively unknown. I...

journal_title：FEMS microbiology letters

authors： Huang KH,Hsu BM,Chou MY,Tsai HL,Kao PM,Wang HJ,Hsiao HY,Su MJ,Huang YL

更新日期：2014-03-01 00:00:00

abstract：:Comparison of sequence data is necessary in older to investigate virus origins, identify features common to virulent strains, and characterize genomic organization within virus families. A virulent caliciviral disease of rabbits recently emerged in China. We have sequenced 1100 bases from the 3' ends of two independen...

journal_title：FEMS microbiology letters

authors： Milton ID,Vlasak R,Nowotny N,Rodak L,Carter MJ

更新日期：1992-05-15 00:00:00

abstract：:Outer membrane-associated proteins of 18 kDa were expressed by enteroaggregative Escherichia coli (EAggEC) belonging to serotypes O126:H27 and O44:H18, which hybridized with a probe derived from a plasmid necessary for enteroaggregative adhesion. The 18 kDa proteins expressed by strains of E. coli, belonging to these ...

journal_title：FEMS microbiology letters

authors： Chart H,Smith HR,Rowe B

更新日期：1995-10-01 00:00:00

abstract：:Research on the porcine respiratory tract pathogen Actinobacillus pleuropneumoniae requires the availability of improved genetic tools. Therefore, using the sacB gene of Bacillus subtilis, we developed a sucrose-based counterselection system that allows rapid curing of an Escherichia coli-A. pleuropneumoniae shuttle v...

journal_title：FEMS microbiology letters

authors： Oswald W,Tonpitak W,Ohrt G,Gerlach G

更新日期：1999-10-01 00:00:00

abstract：:Xylose reductase catalyzes the NAD(P)H-dependent reduction of xylose to xylitol and is essential for growth on xylose by yeasts. To understand the nature of coenzyme binding to the Pichia stipitis xylose reductase, we investigated the role of the strictly conserved Lys270 in the putative IPKS coenzyme binding motif by...

journal_title：FEMS microbiology letters

authors： Kostrzynska M,Sopher CR,Lee H

更新日期：1998-02-01 00:00:00

abstract：:We isolated a fungal strain HS-1 utilizing either ethylene glycol dibenzoate or ethyl benzoate as sole source of carbon and energy, and identified it as Aspergillus nomius, based on morphological and rDNA analyses. An enzyme hydrolyzing the esters was purified from the culture supernatant of the strain to an electroph...

journal_title：FEMS microbiology letters

authors： Uchida H,Kurakata Y,Sawamura H,Inamura N,Kotani T,Uwajima T

更新日期：2003-06-06 00:00:00