Characterization of the gltF gene product of Escherichia coli.

Abstract:

:The glt operon of Escherichia coli comprises the structural genes for the glutamate synthase subunits (gltB and gltD) and gltF, whose product was previously suggested to have regulatory functions. The A/T-rich region between gltD and gltF contains a weak promoter and a translation initiation site for gltF. The GltF protein is preceded by a signal peptide, which is cleaved off during export into the periplasmic space. A gltF::Km(R) insertion mutant was constructed and shown here to have no detectable phenotype with respect to amino acid utilization or ammonium transport. Thus, GltF is apparently not involved in regulation of nitrogen catabolism.

journal_name

FEMS Microbiol Lett

authors

Grassl G,Bufe B,Müller B,Rösel M,Kleiner D

doi

10.1111/j.1574-6968.1999.tb08711.x

keywords:

subject

Has Abstract

pub_date

1999-10-01 00:00:00

pages

79-84

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(99)00397-3

journal_volume

179

pub_type

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