Abstract:
:In the current studies, we used Lineweaver-Burke analysis to examine the role of 1-hydroxybenzotriazole (HBT) in the oxidation of various compounds by laccase from Trametes versicolor. At low concentrations, HBT was a competitive inhibitor of the oxidation, but at high concentrations, it was a noncompetitive inhibitor. Analysis of the oxidation of ferrocytochrome c by the laccase-HBT couple showed that increasing the concentration of ferrocytochrome c did not affect the V(max) but reduced the apparent K(m). In addition, in the manganese peroxidase-Mn(II) reaction, which is a typical oxidation system by mediator, the apparent K(m) and V(max) increased as the concentration of the substrate 2,6-dimethoxyphenol was increased. These results indicate that HBT is involved in the binding of laccase and substrates that laccase cannot oxidize alone.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Hirai H,Shibata H,Kawai S,Nishida Tdoi
10.1111/j.1574-6968.2006.00474.xsubject
Has Abstractpub_date
2006-12-01 00:00:00pages
56-9issue
1eissn
0378-1097issn
1574-6968pii
FML474journal_volume
265pub_type
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journal_title:FEMS microbiology letters
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