Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae.

Abstract:

:Cry4Ba is a delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic delta-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC(50)=140 ng ml(-1)) but virtually inactive to Culex quinquefasciatus larvae. Cyt2Aa2 expressed in E. coli exhibited moderate activity against A. aegypti and C. quinquefasciatus larvae with LC(50) values of 350 and 250 ng ml(-1), respectively. Co-expression of both toxins in E. coli dramatically increased toxicity to both A. aegypti andC. quinquefasciatus larvae (LC(50)=7 and 20 ng ml(-1), respectively). This is the first report to demonstrate that Cry4Ba and Cyt2Aa2 have high synergistic activity against C. quinquefasciatus larvae.

journal_name

FEMS Microbiol Lett

authors

Promdonkoy B,Promdonkoy P,Panyim S

doi

10.1016/j.femsle.2005.08.038

keywords:

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

121-6

issue

1

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(05)00590-2

journal_volume

252

pub_type

杂志文章
  • Microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media.

    abstract::Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02184.x

    authors: Dmitriev VV,Crowley D,Rogachevsky VV,Negri CM,Rusakova TG,Kolesnikova SA,Akhmetov LI

    更新日期:2011-02-01 00:00:00

  • Myo-inositol facilitators IolT1 and IolT2 enhance D-mannitol formation from D-fructose in Corynebacterium glutamicum.

    abstract::Reduction of D-fructose to D-mannitol by whole-cell biotransformation with recombinant resting cells of Corynebacterium glutamicum ATCC13032 requires the coexpression of mdh and fdh, which encode mannitol and formate dehydrogenases, respectively. However, d-mannitol formation is limited by the uptake of d-fructose in ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01425.x

    authors: Bäumchen C,Krings E,Bringer S,Eggeling L,Sahm H

    更新日期:2009-01-01 00:00:00

  • Fungal cytochrome P450s catalyzing hydroxylation of substituted toluenes to form their hydroxymethyl derivatives.

    abstract::The degradation of a series of nitroaromatic compounds by the lignin-degrading fungus Phanerochaete chrysosporium was examined. From 4-nitrotoluene (4-NT), several metabolic intermediates were identified. Initially, 4-NT was converted to 4-nitrobenzyl alcohol (4-NBA), followed by the oxidation reactions to form 4-nitr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.03.036

    authors: Teramoto H,Tanaka H,Wariishi H

    更新日期:2004-05-15 00:00:00

  • Glucose stimulates alginate production and algD transcription in Pseudomonas aeruginosa.

    abstract::A previous study [DeVault et al. (1991) Mol. Microbiol. 5, 2503-2509] suggested that growth of Pseudomonas aeruginosa in glucose-containing medium represses algD gene transcription. In this study, growth of P. aeruginosa in rich medium containing glucose or gluconate increased alginate production and algD transcriptio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10291.x

    authors: Ma JF,Phibbs PV,Hassett DJ

    更新日期:1997-03-15 00:00:00

  • Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger.

    abstract::The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08393.x

    authors: Kirimura K,Matsui T,Sugano S,Usami S

    更新日期:1996-08-01 00:00:00

  • A novel prophage lysin Ply5218 with extended lytic activity and stability against Streptococcus suis infection.

    abstract::Streptococcus suis (S. suis) is an emerging zoonotic agent that exhibits high level resistance to classic antibiotics and a heavy burden in the swine industry. Therefore alternative antibacterial agents need to be developed. A novel endolysin derived from the S. suis temperate phage phi5218, termed Ply5218, was identi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnw186

    authors: Zhang H,Zhang C,Wang H,Yan YX,Sun J

    更新日期:2016-09-01 00:00:00

  • Rhizobium leguminosarum bv. trifolii rosR gene expression is regulated by catabolic repression.

    abstract::Rhizobium leguminosarum bv. trifolii rosR gene encodes a transcriptional regulator involved in the positive regulation of exopolysaccharide synthesis. Transcription of rosR is directed by two promoters, distal P1 and proximal P2, of different strengths. We demonstrated that rosR P1 functions as the main promoter and, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2008.01443.x

    authors: Janczarek M,Skorupska A

    更新日期:2009-02-01 00:00:00

  • Comparison of transcription of multiple genes at three developmental stages of the plant pathogen Sclerotinia sclerotiorum.

    abstract::The ascomycete Sclerotinia sclerotiorum is a plant pathogen with a very broad host range. In order to identify and characterize genes involved in S. sclerotiorum infection of Brassica napus (canola), expressed sequence tags (ESTs) were examined from libraries prepared from three tissues: complex appressorium (infectio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00212.x

    authors: Sexton AC,Cozijnsen AJ,Keniry A,Jewell E,Love CG,Batley J,Edwards D,Howlett BJ

    更新日期:2006-05-01 00:00:00

  • Plasmid and chromosomal DNA recovery by electroextraction of cyanobacteria.

    abstract::High voltage electroporation has been investigated as a method for rapid recovery of plasmid and chromosomal DNA from the cyanobacteria Nostoc PCC 7121, Synechococcus PCC 7002, and Anabaena PCC 7120. Pulses of 18 kV/cm and higher applied to concentrated Nostoc cells carrying a shuttle plasmid (pRL25) resulted in copio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07541.x

    authors: Moser D,Zarka D,Hedman C,Kallas T

    更新日期:1995-05-15 00:00:00

  • Linkage between cellular adherence and biofilm formation in Escherichia coli O157:H7 EDL933.

    abstract::During the establishment of Escherichia coli O157:H7 infection, its capacity to adhere to host intestinal epithelial cells is the critical first step in pathogenesis. It also has the capability to form biofilms, and because both are surface activities, we sought to gain insight into a potential linkage between biofilm...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02173.x

    authors: Puttamreddy S,Minion FC

    更新日期:2011-02-01 00:00:00

  • Genetic analysis of Carboxydothermus hydrogenoformans carbon monoxide dehydrogenase genes cooF and cooS.

    abstract::Carboxydothermus hydrogenoformans is an extremely thermophilic, Gram-positive bacterium growing on carbon monoxide (CO) as single carbon and energy source and producing only H(2) and CO(2). Carbon monoxide dehydrogenase is a key enzyme for CO metabolism. The carbon monoxide dehydrogenase genes cooF and cooS from C. hy...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09346.x

    authors: González JM,Robb FT

    更新日期:2000-10-15 00:00:00

  • Mutation in dipZ leads to reduced production of active human placental alkaline phosphatase in Escherichia coli.

    abstract::We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(94)90251-8

    authors: Beck R,Crooke H,Jarsch M,Cole J,Burtscher H

    更新日期:1994-12-01 00:00:00

  • Sequencing and characterization of a novel serine metalloprotease from Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei, a Gram-negative bacterium is found in the soil and water, mainly in Southeast Asia and Northern Australia. It is responsible for melioidosis in human and animals. The bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. The is...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09360.x

    authors: Lee MA,Liu Y

    更新日期:2000-11-01 00:00:00

  • Biogenic amine-forming microbial communities in cheese.

    abstract::The aim of this study was to screen two cheese starter cultures and cheese-borne microbial communities with the potential to produce biogenic amines in cheese during ripening. Bacteria of the genera Enterococcus and Lactobacillus and coliform bacteria were isolated from Dutch-type semi-hard cheese at the beginning of ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00922.x

    authors: Burdychova R,Komprda T

    更新日期:2007-11-01 00:00:00

  • Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae.

    abstract::Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: How...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13460.x

    authors: Bricker AL,Camilli A

    更新日期:1999-03-15 00:00:00

  • Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei.

    abstract::Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00916-9

    authors: Diener SE,Dunn-Coleman N,Foreman P,Houfek TD,Teunissen PJ,van Solingen P,Dankmeyer L,Mitchell TK,Ward M,Dean RA

    更新日期:2004-01-30 00:00:00

  • Induction of laccase activity in the edible straw mushroom, Volvariella volvacea.

    abstract::Volvariella volvacea, strain V14, produces multiple forms of extracellular laccase when grown in submerged culture in a defined medium with glucose as sole carbon source, and on cotton waste 'compost' representative of the conditions used for industrial-scale mushroom cultivation. In liquid culture, enzyme synthesis i...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2003.tb11510.x

    authors: Chen S,Ma D,Ge W,Buswell JA

    更新日期:2003-01-21 00:00:00

  • IdeE, an IgG-endopeptidase of Streptococcus equi ssp. equi.

    abstract::Streptococcus equi ssp. equi is the causative agent of strangles, a highly contagious and serious disease in the upper respiratory tract of horses. The present study describes the characterization of IdeE, a homolog of the secreted IgG-specific protease IdeS/Mac of Streptococcus pyogenes. The activity of IdeE is compa...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00404.x

    authors: Lannergård J,Guss B

    更新日期:2006-09-01 00:00:00

  • Structure of the Escherichia coli 0104 polysaccharide and its identity with the capsular K9 polysaccharide.

    abstract::The 0104 antigen (lipopolysaccharide, LPS) of Escherichia coli has an acidic O specific polysaccharide. From the aqueous phase of a phenol water extraction of E. coli O104: K-, a fraction was obtained by ultracentrifugation and Cetavlon precipitation of the supernatant, which was enriched in long-chain LPS. Compositio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90673-c

    authors: Kogan G,Jann B,Jann K

    更新日期:1992-03-01 00:00:00

  • RD1 region in mycobacterial genome is involved in the induction of necrosis in infected RAW264 cells via mitochondrial membrane damage and ATP depletion.

    abstract::It was shown that virulent Mycobacterium tuberculosis H37Rv induces necrosis of infected RAW264 cells at 24 h post infection while avirulent H37Ra and an attenuated H37Rv mutant that is deficient for RD1 region (H37RvDeltaRD1) cause less necrosis of the infected cells. While H37Rv caused damage of the mitochondrial in...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00838.x

    authors: Kaku T,Kawamura I,Uchiyama R,Kurenuma T,Mitsuyama M

    更新日期:2007-09-01 00:00:00

  • Development of a gene knockout system for Ralstonia eutropha H16 based on the broad-host-range vector expressing a mobile group II intron.

    abstract::Ralstonia eutropha H16 is a Gram-negative lithoautotrophic bacterium and is one of the best biopolymer-producing bacteria. It can grow to high cell densities either under lithoautotrophic or under heterotrophic conditions, which makes it suitable for a number of biotechnological applications. Also, R. eutropha H16 can...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02041.x

    authors: Park JM,Jang YS,Kim TY,Lee SY

    更新日期:2010-08-01 00:00:00

  • Escherichia coli strain Nissle 1917-from bench to bedside and back: history of a special Escherichia coli strain with probiotic properties.

    abstract::Among the gram-negative microorganisms with probiotic properties, Escherichia coli strain Nissle 1917 (briefly EcN) is probably the most intensively investigated bacterial strain today. Since nearly 100 years, the EcN strain is used as the active pharmaceutical ingredient in a licensed medicinal product that is distri...

    journal_title:FEMS microbiology letters

    pub_type: 历史文章,杂志文章,评审

    doi:10.1093/femsle/fnw212

    authors: Sonnenborn U

    更新日期:2016-10-01 00:00:00

  • Biological activity of the azlactone derivative EPA-35 against Trypanosoma cruzi.

    abstract::Chagas disease, caused by Trypanosoma cruzi, affects six to seven million people worldwide. Treatment is based on benznidazole, producing several side effects and debatable efficacy, highlighting the need for new alternative drugs. We investigated the activity of four C-4 functionalized azlactone derivatives (EPA-27, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx020

    authors: de Azeredo CM,Ávila EP,Pinheiro DL,Amarante GW,Soares MJ

    更新日期:2017-02-01 00:00:00

  • Characterization of dextran-producing Weissella strains isolated from sourdoughs and evidence of constitutive dextransucrase expression.

    abstract::The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02067.x

    authors: Bounaix MS,Robert H,Gabriel V,Morel S,Remaud-Siméon M,Gabriel B,Fontagné-Faucher C

    更新日期:2010-10-01 00:00:00

  • Comparison of the thickening properties of four Lactobacillus delbrueckii subsp. bulgaricus strains and physicochemical characterization of their exopolysaccharides.

    abstract::It is now well established that physicochemical properties of exopolysaccharides (EPS) can vary between strains of a given species and according to growth conditions. The EPS production of four strains of Lactobacillus bulgaricus was monitored during growth in milk and in a chemically defined media. All strains, inclu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00214-3

    authors: Petry S,Furlan S,Waghorne E,Saulnier L,Cerning dagger J,Maguin E

    更新日期:2003-04-25 00:00:00

  • The immune response to Lactococcus lactis: implications for its use as a vaccine delivery vehicle.

    abstract::The development of natural antibodies to Lactococcus lactis in three inbred strains of mice and the effect of inoculating L. lactis into these mice has been investigated. All animals developed detectable levels of natural (systemic and secretory) anti-lactococcal antibodies. Systemic anti-lactococcal antibodies were p...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07041.x

    authors: Norton PM,Brown HW,Le Page RW

    更新日期:1994-07-15 00:00:00

  • Bacteria associated with arbuscular mycorrhizal fungi within roots of plants growing in a soil highly contaminated with aliphatic and aromatic petroleum hydrocarbons.

    abstract::Arbuscular mycorrhizal fungi (AMF) belong to phylum Glomeromycota, an early divergent fungal lineage forming symbiosis with plant roots. Many reports have documented that bacteria are intimately associated with AMF mycelia in the soil. However, the role of these bacteria remains unclear and their diversity within intr...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12533

    authors: Iffis B,St-Arnaud M,Hijri M

    更新日期:2014-09-01 00:00:00

  • Bacterial gamma-glutamyltranspeptidases: comparison of Escherichia coli and Pseudomonas gamma-glutamyltranspeptidase.

    abstract::A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90342-l

    authors: Ishiye M,Niwa M

    更新日期:1992-10-15 00:00:00

  • Outer membrane proteins of gentamicin induced small colony variants of Pseudomonas aeruginosa.

    abstract::Small colony variants (SCVs) of Pseudomonas aeruginosa NCTC 6750 (WT) were repeatedly isolated in an in vitro kinetic model after exposure to gentamicin (GM). There were minor differences biochemically and in phage and serotyping between the wild type (WT) strain and SCVs. Changes in outer membrane protein profiles we...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90165-1

    authors: Langford PR,Anwar H,Gonda I,Brown MR

    更新日期:1989-10-01 00:00:00

  • Diversity of endophytic fungi of Paeonia lactiflora Pallas and screening for fungal paeoniflorin producers.

    abstract::Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny263

    authors: Cheng X,Wei Z,Pu S,Xiang M,Yan A,Zhang Y,Wang X

    更新日期:2018-12-01 00:00:00