Abstract:
:Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits and internal transcribed spacers sequences, indicating that endophytic fungi of P. lactiflora are abundant and diverse. The dominant endophytic fungi were Aspergillus, Alternaria and Penicillium. More fungi were recovered from leaves than from roots and stems. The similarity index was highest between the stems and leaves (0.733), followed by the roots and leaves (0.615) and the stems and roots (0.563). Analyses of the fermentation extracts of 22 endophytic fungi by high-performance liquid chromatography and mass spectrometry revealed that three strains (R12, Alternaria tenuissima; S4, Aspergillus flavus; and R17 Penicillium commune) were able to produce paeoniflorin. Among the paeoniflorin-producing fungi, the yield of paeoniflorin from A. flavus S4 was 342.4 μg/L, and this strain could be used as a candidate for the industrial production of paeoniflorin.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Cheng X,Wei Z,Pu S,Xiang M,Yan A,Zhang Y,Wang Xdoi
10.1093/femsle/fny263subject
Has Abstractpub_date
2018-12-01 00:00:00issue
24eissn
0378-1097issn
1574-6968pii
5184457journal_volume
365pub_type
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