Fluorescence based measurements of Fusobacterium nucleatum coaggregation and of fusobacterial attachment to mammalian cells.

abstract：:Endophytic fungi colonize plants without causing symptoms of disease and can enhance the resistance of their host to pathogens. We cultivated 53 fungal strains from wild lima bean (Phaseolus lunatus) and investigated their effects on pathogens using in vitro assays and experiments in planta. Most strains were annotate...

journal_title：FEMS microbiology letters

authors： Adame-Álvarez RM,Mendiola-Soto J,Heil M

更新日期：2014-06-01 00:00:00

abstract：:The mosquitocidal crystal protein, Cry19Aa, from Bacillus thuringiensis ssp. jegathesan, has high toxicity to Anopheles stephensi and Culex pipiens but is less toxic to Aedes aegypti. To study the functional role of putative domain II and surface residues in mosquito toxicity, 16 alanine substitution mutations were in...

journal_title：FEMS microbiology letters

authors： Roh JY,Nair MS,Liu XS,Dean DH

更新日期：2009-06-01 00:00:00

abstract：:A gene product with an apparent molecular mass of approximately 39,000 Da can be identified in the cytoplasmic membrane of Escherichia coli upon expression of cloned envC. In this communication we report that the product was labelled with [3H]glycerol and [3H]palmitic acid, and a precursor molecule of increased molecu...

journal_title：FEMS microbiology letters

authors： Seiffer D,Klein JR,Plapp R

更新日期：1993-03-01 00:00:00

abstract：:Brucella usually carry two highly homologous genes (omp2a and omp2b) for porin-like proteins. In several B. abortus biovars the omp2a gene has a large deletion compared to other Brucella omp2's. In this study we have measured Omp2 pore activity in planar bilayers. Omp2b exhibits well-defined trimeric channel activity ...

journal_title：FEMS microbiology letters

authors： Mobasheri H,Ficht TA,Marquis H,Lea EJ,Lakey JH

更新日期：1997-10-01 00:00:00

abstract：:Changes in gene expression play a critical role in enhancing the ability of cyanobacteria to survive under cold conditions. In the present study, Spirulina platensis cultures were grown at the optimal growth temperature, in the light, before being transferred to dark conditions at 22 degrees C. Two dimensional-differe...

journal_title：FEMS microbiology letters

authors： Jeamton W,Mungpakdee S,Sirijuntarut M,Prommeenate P,Cheevadhanarak S,Tanticharoen M,Hongsthong A

更新日期：2008-04-01 00:00:00

abstract：:Lactobacillus johnsonii La1 (La1) is a probiotic strain capable of stimulating the immune system of the host and interfering with gastrointestinal pathogens. This study evaluates how the ingestion of different amounts of La1 influences the main bacterial populations of the fecal microbiota. Eight asymptomatic voluntee...

journal_title：FEMS microbiology letters

authors： Garrido D,Suau A,Pochart P,Cruchet S,Gotteland M

更新日期：2005-07-15 00:00:00

abstract：:To determine the existence of an acid stress response in Helicobacter pylori the global changes in the proteins synthesized by the bacterium when subjected to an acid stress were studied. H. pylori ATCC43504 previously adapted to pH 7 did not show an acid stress response as detected by the two-dimensional electrophore...

journal_title：FEMS microbiology letters

authors： Toledo H,Valenzuela M,Rivas A,Jerez CA

更新日期：2002-07-16 00:00:00

abstract：:Sugar alcohols (polyols) have important roles as nutrients, anti-freezing agents and scavengers of free radicals in cold-adapted bacteria, but the characteristics of polyol dehydrogenases in cold-adapted bacteria remain largely unknown. In this study, based on the observation that a cold-adapted bacterium Pseudomonas ...

journal_title：FEMS microbiology letters

authors： DangThu Q,Nguyen TT,Jang SH,Lee C

更新日期：2021-01-05 00:00:00

abstract：:Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci. Three of the isolates behaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium-supplied t...

journal_title：FEMS microbiology letters

authors： McClarty G,Fan H,Andersen AA

更新日期：1993-04-15 00:00:00

abstract：:The chrysene-degrading bacterium Pseudoxanthomonas sp. PNK-04 was isolated from a coal sample. Three novel metabolites, hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid and salicylic acid, were identified by TLC, HPLC and MS. Key enzyme activities, namely 1-hydroxy-2-naphthoate hydroxylase, 1,2-dihydroxynaphthalen...

journal_title：FEMS microbiology letters

authors： Nayak AS,Sanjeev Kumar S,Santosh Kumar M,Anjaneya O,Karegoudar TB

更新日期：2011-07-01 00:00:00

abstract：:Quantitative cloning efficiencies for B. megaterium, B. subtilis, and E. coli were compared. Transformation of B. megaterium is less efficient than transformation of B. subtilis or E. coli. The frequency of recombinant clones was equal in E. coli and B. megaterium; both somewhat higher than in B. subtilis. Equivalent ...

journal_title：FEMS microbiology letters

authors： Von Tersch MA,Robbins HL

更新日期：1990-08-01 00:00:00

abstract：:A newly isolated strain of Variovorax paradoxus could grow on homovanillate and several monohydroxylated phenylacetic acids. During growth on homovanillate, the organism formed separate NAD(P)H-dependent hydroxylases with activity towards 4-hydroxyphenylacetic acid and homovanillate. Homovanillate hydroxylase catalyse...

journal_title：FEMS microbiology letters

authors： Allison N,Turner JE,Wait R

更新日期：1995-12-15 00:00:00

abstract：:A red-pigmented, Gram-negative, motile, strictly aerobic, mesophilic, oval- or short rod-shaped bacterium (TDMA-17(T)) was isolated from fresh water collected at Misasa, a radioactive site in Japan. TDMA-17(T) was slightly tolerant against gamma-ray irradiation, and effectively produced carotenoids (2.8 mg g(-1) dry c...

journal_title：FEMS microbiology letters

authors： Asker D,Beppu T,Ueda K

更新日期：2007-08-01 00:00:00

abstract：:The use of plasmids possessing a regulatable gene coding for a site-specific recombinase together with its recognition sequences significantly facilitates genome manipulations since it allows self-excision of the portion of the genetic construct integrated into the host genome. Stable maintenance of such plasmids in E...

journal_title：FEMS microbiology letters

authors： Agaphonov MO

更新日期：2017-12-01 00:00:00

abstract：:The enterobacterium Photorhabdus luminescens produces a number of toxins to kill its insect host. By analyzing the genomic sequence of P. luminescens TT01, we found that amino acid sequences encoded by plu1961 and plu1962 showed high similarity to XaxAB binary toxin of Xenorhabuds nematophila, which has both necrotic ...

journal_title：FEMS microbiology letters

authors： Zhang X,Hu X,Li Y,Ding X,Yang Q,Sun Y,Yu Z,Xia L,Hu S

更新日期：2014-01-01 00:00:00

abstract：:Streptococcus suis (S. suis) is an emerging zoonotic agent that exhibits high level resistance to classic antibiotics and a heavy burden in the swine industry. Therefore alternative antibacterial agents need to be developed. A novel endolysin derived from the S. suis temperate phage phi5218, termed Ply5218, was identi...

journal_title：FEMS microbiology letters

authors： Zhang H,Zhang C,Wang H,Yan YX,Sun J

更新日期：2016-09-01 00:00:00

abstract：:Klebsiella aerogenes W70 was reported previously to produce a membrane-associated tyramine oxidase (TynA) that did not act on 2-phenylethylamine. Subsequently, a gene cloned from K. aerogenes W70 produced a soluble amine oxidase (MaoA) that acted readily on 2-phenylethylamine and tyramine. This enzyme appeared to be e...

journal_title：FEMS microbiology letters

authors： Cooper RA

更新日期：1997-01-01 00:00:00

abstract：:Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (FADB::...

journal_title：FEMS microbiology letters

authors： Hang X,Lin Z,Chen J,Wang G,Hong K,Chen GQ

更新日期：2002-06-18 00:00:00

abstract：:We recently described adhesion to and invasion of bovine mammary gland cells by Staphylococcus aureus in vitro. Here, we show that the levels of adhesion and invasion are dependent on the bacterial growth phase and are controlled by the agr locus. Incubation of exponential growth phase cells of S. aureus with mammary ...

journal_title：FEMS microbiology letters

authors： Lammers A,Nuijten PJ,Smith HE

更新日期：1999-11-01 00:00:00

abstract：:The emergence of antibiotic resistance has necessitated new therapeutic approaches for combating persistent bacterial infection. An alternative approach is regulation of bacterial virulence instead of growth suppression, which can readily lead to drug resistance. The virulence of the opportunistic human pathogen Pseud...

journal_title：FEMS microbiology letters

authors： Lee JH,Kim YG,Cho MH,Kim JA,Lee J

更新日期：2012-04-01 00:00:00

abstract：:Haemophilus influenzae has an absolute growth requirement for heme and a heme binding lipoprotein (HbpA) has been implicated in the utilization of this essential nutrient. HbpA was identified by examining clones from an H. influenzae genomic library that caused Escherichia coli harboring the clone to bind heme. Howeve...

journal_title：FEMS microbiology letters

authors： Morton DJ,Madore LL,Smith A,Vanwagoner TM,Seale TW,Whitby PW,Stull TL

更新日期：2005-12-15 00:00:00

abstract：:beta-Glucosidase in Aspergillus nidulans was found to be both intracellular and extracellular. The intracellular beta-glucosidase was synthesized after the exhaustion of carbon source in the medium. The extracellular enzyme appeared with autolysis of the mycelium. Biosynthesis of beta-glucosidase was not induced by va...

journal_title：FEMS microbiology letters

authors： Lee J,Kwon KS,Hah YC

更新日期：1996-01-01 00:00:00

abstract：:We present evidence that the anthrax toxin lethal factor binds multiple zinc atoms. Results from atomic adsorption spectroscopy indicate that lethal factor contains approximately three zinc atoms per toxin molecule. Lethal factor treated with EDTA and o-phenanthroline contained a similar number of zinc atoms, indicati...

journal_title：FEMS microbiology letters

authors： Kochi SK,Schiavo G,Mock M,Montecucco C

更新日期：1994-12-15 00:00:00

abstract：:The physiology of the response in the methanotrophic bacterium Methylococcus capsulatus Bath towards thermal and solvent stress was studied. A systematic investigation of the toxic effects of organic compounds (chlorinated phenols and alkanols) on the growth of this bacterium was carried out. The sensitivity to the te...

journal_title：FEMS microbiology letters

authors： Löffler C,Eberlein C,Mäusezahl I,Kappelmeyer U,Heipieper HJ

更新日期：2010-07-01 00:00:00

abstract：:The mechanism of adaptation of bacteria to survive at elevated temperature in the human host and the expression of heat-shock proteins in response to stress was examined by labelling with [35S]methionine. An increase in culture temperature from 26 degrees C to 37 degrees C induced expression of certain bacterial prote...

journal_title：FEMS microbiology letters

authors： Salotra P,Singh DK,Seal KP,Krishna N,Jaffe H,Bhatnagar R

更新日期：1995-08-15 00:00:00

abstract：:A critical step in the process of metagenome analysis is to screen for clones that contain specific genes among a large number of clones. To form one of the sequence-based screening tools of a metagenome library, we designed a format of microarray [metagenome microarray (MGA)] that is arrayed with fosmid library clone...

journal_title：FEMS microbiology letters

authors： Park SJ,Kang CH,Chae JC,Rhee SK

更新日期：2008-07-01 00:00:00

abstract：:Culture medium from an isolate of the fungus Aspergillus candidus was extracted, fractionated and examined to discover compounds antagonistic to plant-parasitic nematodes that are important pathogens of agricultural crops. Column, thin layer and preparative chromatographies and spectral and elemental analyses, were us...

journal_title：FEMS microbiology letters

authors： Shemshura ON,Bekmakhanova NE,Mazunina MN,Meyer SL,Rice CP,Masler EP

更新日期：2016-03-01 00:00:00

abstract：:The ascomycetous black yeasts Hortaea werneckii, Phaeotheca triangularis, and Trimmatostroma salinum are halophilic fungi that inhabit hypersaline water of solar salterns. They are characterized by slow, meristematic growth and very thick, darkly pigmented cell walls. The dark pigment, generally thought to be melanin,...

journal_title：FEMS microbiology letters

authors： Kogej T,Wheeler MH,Lanisnik Rizner T,Gunde-Cimerman N

更新日期：2004-03-19 00:00:00

abstract：:Bacillus anthracis can be identified on the basis of the detection of virulence factor genes located on two plasmids, pXO1 and pXO2. Thus isolates lacking both pXO1 and pXO2 are indistinguishable from closely related B. cereus group bacteria. We developed a multiplex PCR assay for characterization of B. anthracis isol...

journal_title：FEMS microbiology letters

authors： Ramisse V,Patra G,Garrigue H,Guesdon JL,Mock M

更新日期：1996-11-15 00:00:00

abstract：:The acquisition of tolerance to high hydrostatic pressure of 220 MPa (HHP) in response to a 0.4 mM hydrogen peroxide, 6% ethanol and cold-shock (10 degrees C) pretreatment for different lengths of times was studied in the yeast Saccharomyces cerevisiae. The protection conferred by these different treatments was simila...

journal_title：FEMS microbiology letters

authors： Palhano FL,Orlando MT,Fernandes PM

更新日期：2004-04-01 00:00:00