Abstract:
:Brucella usually carry two highly homologous genes (omp2a and omp2b) for porin-like proteins. In several B. abortus biovars the omp2a gene has a large deletion compared to other Brucella omp2's. In this study we have measured Omp2 pore activity in planar bilayers. Omp2b exhibits well-defined trimeric channel activity whilst Omp2a forms monomeric pores of variable size which are smaller than Omp2b. No sequence homology exists between Omp2 and porins of known structure, so hydrophobic moment analysis has been used to model their membrane topology. From this it appears likely that the deletion removes the crucial L3 internal loop.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Mobasheri H,Ficht TA,Marquis H,Lea EJ,Lakey JHdoi
10.1111/j.1574-6968.1997.tb12681.xsubject
Has Abstractpub_date
1997-10-01 00:00:00pages
23-30issue
1eissn
0378-1097issn
1574-6968pii
S0378-1097(97)00341-8journal_volume
155pub_type
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