Comparison of Mycoplasma agalactiae isolates by pulsed field gel electrophoresis, SDS-PAGE and immunoblotting.

Abstract:

:We have analyzed 81 isolates of Mycoplasma agalactiae from four different regions of Italy between 1990 and 1995 in order to identify antigenic differences through SDS-PAGE and Western blotting and chromosomal DNA restriction endonuclease cleavage pattern differences. Antigenic variability in M. agalactiae isolates was investigated analyzing hydrophobic membrane protein fractions by immunoblotting using pooled sheep antiserum from naturally infected sheep. Large restriction fragments obtained cleaving genomic DNAs with SmaI, NruI, SalI, XhoI, BssHII and KpnI were analyzed by pulsed field gel electrophoresis. Genetic analysis indicates that isolates are all similar without intraspecific differences. This homogeneity was confirmed by immunoblotting: 80 and 50 kDa antigens are present in all strains analyzed.

journal_name

FEMS Microbiol Lett

authors

Tola S,Idini G,Manunta D,Casciano I,Rocchigiani AM,Angioi A,Leori G

doi

10.1111/j.1574-6968.1996.tb08490.x

subject

Has Abstract

pub_date

1996-10-01 00:00:00

pages

259-65

issue

2-3

eissn

0378-1097

issn

1574-6968

journal_volume

143

pub_type

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