Expression of a new porin 'OmpE' by strains of Salmonella enteritidis.


:Strains of Salmonella enteritidis expressed a novel porin with a subunit size of 35.5 kDa as shown by SDS-PAGE. This protein was expressed as a major outer membrane protein (MOMP) when grown on nutrient agar, McConkey agar or blood agar, or in Tris-succinate medium; but was only produced in trace amounts when strains were grown in nutrient broth. This OMP was produced by all strains of S. enteritidis examined, regardless of phage type, and expression was not related to the possession of a 38-MDa mouse-virulence plasmid or the ability of strains to make long-chain lipopolysaccharide. This new porin has been tentatively called OmpE.


FEMS Microbiol Lett


Chart H,Frost JA,Rowe B




Has Abstract


1993-05-15 00:00:00












  • The alternative sigma factor RpoN regulates the quorum sensing gene rhlI in Pseudomonas aeruginosa.

    abstract::The rhl quorum sensing (QS) circuit of Pseudomonas aeruginosa is known to regulate the expression of a number of virulence factors. This study investigates the regulation of rhlI, encoding the auto-inducer synthase RhlI responsible for the synthesis of N-butryl-L-homoserine lactone (BHL). A putative RpoN binding site ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Thompson LS,Webb JS,Rice SA,Kjelleberg S

    更新日期:2003-03-28 00:00:00

  • Halogenation of aromatic compounds: thermodynamic, mechanistic and ecological aspects.

    abstract::Biological halogenation of aromatic compounds implies the generation of reducing equivalents in the form of e.g. NADH. Thermodynamic calculations show that coupling the halogenation step to a step in which the reducing equivalents are oxidized with a potent oxidant such as O2 or N2O makes the halogenation reaction the...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Dolfing J

    更新日期:1998-10-15 00:00:00

  • Identification of Pantoea ananatis gene encoding membrane pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase and pqqABCDEF operon essential for PQQ biosynthesis.

    abstract::Pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. Computer analysis of the P. ananatis SC17(0) sequenced genome revealed an ORF encoding a homologue (named gcd) of the mGDH (EC apoenzyme from Escherichia coli and a putative pyrroloquinoline quinone (PQQ) biosynthetic o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Andreeva IG,Golubeva LI,Kuvaeva TM,Gak ER,Katashkina JI,Mashko SV

    更新日期:2011-05-01 00:00:00

  • Inhibition of peptidoglycan biosynthesis in Bacillus megaterium by daptomycin.

    abstract::The effects of daptomycin on exponential phase cells of Bacillus megaterium were investigated. Bacteriostasis was observed for concentrations between 1 and 3 micrograms/ml and maximal rate of cell lysis at 10 micrograms/ml. At sublytic concentrations (1.5-3 micrograms/ml), the variations of the pools of UDP-N-acetylgl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Mengin-Lecreulx D,Allen NE,Hobbs JN,van Heijenoort J

    更新日期:1990-06-01 00:00:00

  • luxS in bacteria isolated from 25- to 40-million-year-old amber.

    abstract::Interspecies bacterial communication is mediated by autoinducer-2, whose synthesis depends on luxS. Due to the apparent universality of luxS (present in more than 40 bacterial species), it may have an ancient origin; however, no direct evidence is currently available. We amplified luxS in bacteria isolated from 25- to...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Santiago-Rodriguez TM,Patrício AR,Rivera JI,Coradin M,Gonzalez A,Tirado G,Cano RJ,Toranzos GA

    更新日期:2014-01-01 00:00:00

  • Diversity of chromosomal AmpC beta-lactamases from Enterobacter cloacae isolates in a Portuguese hospital.

    abstract::Six clinical isolates of Enterobacter cloacae isolated in a Portuguese hospital, between April 1999 and November 2000, demonstrated resistance to almost all broad-spectrum cephalosporins, except to cefepime. These isolates were susceptible to quinolones and to aminoglycosides. Isoelectric focusing demonstrated product...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Conceição T,Faria N,Lito L,Melo Cristino J,Salgado MJ,Duarte A

    更新日期:2004-01-30 00:00:00

  • Pseudomonas aeruginosa Trent and zinc homeostasis.

    abstract::Pseudomonas aeruginosa is a Gram-negative pathogen and the major cause of mortality in patients with cystic fibrosis. The mechanisms that P. aeruginosa strains use to regulate intracellular zinc have an effect on infection, antibiotic resistance and the propensity to form biofilms. However, zinc homeostasis in P. aeru...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Davies CB,Harrison MD,Huygens F

    更新日期:2017-09-01 00:00:00

  • Determination of viridans streptococci surface lipoteichoic acid by enzyme linked immune sorbent assay.

    abstract::An enzyme linked immune sorbent assay (ELISA) was developed to measure bacterial surface lipoteichoic acid (LTA). Numerous strains of oral streptococci belonging to the 'viridans' group were examined on three separate occasions. The results show that, under these cultural conditions, oral streptococci do not normally ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hogg SD,Manning JE

    更新日期:1989-04-01 00:00:00

  • Characterization of SCAR markers of Eimeria spp. of domestic fowl and construction of a public relational database (The Eimeria SCARdb).

    abstract::This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all mark...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fernandez S,Katsuyama AM,Kashiwabara AY,Madeira AM,Durham AM,Gruber A

    更新日期:2004-09-01 00:00:00

  • Genome-wide identification, characterization and expression analysis of lineage-specific genes within Hanseniaspora yeasts.

    abstract::Lineage-specific genes (LSGs) are defined as genes with sequences that are not significantly similar to those in any other lineage. LSGs have been proposed, and sometimes shown, to have significant effects in the evolution of biological function. In this study, two sets of Hanseniaspora spp. LSGs were identified by co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Chen K,Tian Z,Chen P,He H,Jiang F,Long CA

    更新日期:2020-06-01 00:00:00

  • Coordination of export and glycosylation of landomycins in Streptomyces cyanogenus S136.

    abstract::In Streptomyces cyanogenus S136 gene cluster for biosynthesis of polyglycosylated angucycline landomycin A (LaA), a divergently oriented gene pair for a TetR-family regulator (lanK) and an efflux protein (lanJ) is located, whose functions remained obscure. Overexpression and disruption studies showed that lanK and lan...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ostash I,Ostash B,Luzhetskyy A,Bechthold A,Walker S,Fedorenko V

    更新日期:2008-08-01 00:00:00

  • Phase I and phase II enzymes produced by Cunninghamella elegans for the metabolism of xenobiotics.

    abstract::The filamentous fungus Cunninghamella elegans has the ability to metabolize xenobiotics, including polycyclic aromatic hydrocarbons and pharmaceutical drugs, by both phase I and II biotransformations. Cytosolic and microsomal fractions were assayed for activities of cytochrome P450 monooxygenase, aryl sulfotransferase...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Zhang D,Yang Y,Leakey JE,Cerniglia CE

    更新日期:1996-05-01 00:00:00

  • The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes.

    abstract::Brucella abortus B19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. B19 differs from other B. abortus strains in its sensitivity to erythritol. We took advantage of a previously obtained erythritol sensitive Tn5 insertion mutant of B. abortu...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Sangari FJ,García-Lobo JM,Agüero J

    更新日期:1994-09-01 00:00:00

  • Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7.

    abstract::The molecular mechanisms controlling expression of the long polar fimbriae 2 (Lpf2) of enterohemorrhagic Escherichia coli (EHEC) O157:H7 were evaluated. Primer extension was used to locate the lpfA2 transcriptional start site in EHEC strain EDL933 at 171 bp upstream of the lpfA2 start codon. Semi-quantitative RT-PCR d...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Arenas-Hernández MM,Rojas-López M,Medrano-López A,Nuñez-Reza KJ,Puente JL,Martínez-Laguna Y,Torres AG

    更新日期:2014-08-01 00:00:00

  • Construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene.

    abstract::A gene fusion between the Saccharomyces cerevisiae actin gene promoter and the cDNA of the Fusarium solani f. sp. pisi pelA gene has been constructed. This expression cassette has been introduced into the industrial wine yeast strain T73. The resulting recombinant strain is able to secrete active PELA enzyme into the ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: González-Candelas L,Cortell A,Ramon D

    更新日期:1995-03-01 00:00:00

  • The IS1 elements in Shigella boydii: horizontal transfer, vertical inactivation and target duplication.

    abstract::IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E. coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E. coli O157:H7 and K12. T...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Hsu WB,Chen JH

    更新日期:2003-05-28 00:00:00

  • Roles of siderophore in manganese-oxide reduction by Shewanella oneidensis MR-1.

    abstract::Dissimilatory metal-reducing bacteria (DMRB), such as Shewanella oneidensis MR-1, are of great interest for their importance in the biogeochemical cycling of metals and utility in biotechnological processes, such as bioremediation and microbial fuel cells. To identify genes necessary for metal reduction, this study co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kouzuma A,Hashimoto K,Watanabe K

    更新日期:2012-01-01 00:00:00

  • Expression of the short fimbriae of Porphyromonas gingivalis is regulated in oral bacterial consortia.

    abstract::The Mfa1 protein of Porphyromonas gingivalis is the structural subunit of the short fimbriae and mediates coadhesion between P. gingivalis and Streptococcus gordonii. We utilized a promoter-lacZ reporter construct to examine the regulation of mfa1 expression in consortia with common oral plaque bacteria. Promoter acti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park Y,James CE,Yoshimura F,Lamont RJ

    更新日期:2006-09-01 00:00:00

  • Structure and properties of novel inclusions in Shewanella putrefaciens.

    abstract::Cytoplasmic inclusions surrounded by a bilayer membrane were seen in thin sections. negatively stained and freeze-fractured preparations of Shewanella putrefaciens. Cells harvested from the late exponential and early stationary phase showed a higher number of these vesicles than bacteria isolated from early exponentia...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Krause B,Beveridge TJ,Remsen CC,Nealson KH

    更新日期:1996-05-15 00:00:00

  • Description of Sulfitobacter sediminilitoris sp. nov., isolated from a tidal flat.

    abstract::A Gram-stain-negative bacterial strain, JBTF-M27T, was isolated from a tidal flat from Yellow Sea, Republic of Korea. Neighbor-joining phylogenetic tree of 16S rRNA gene sequences showed that strain JBTF-M27T fell within the clade comprising the type strains of Sulfitobacter species. Strain JBTF-M27T exhibited the hig...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Park S,Chen S,Lee JS,Kim W,Yoon JH

    更新日期:2020-05-01 00:00:00

  • Nitrogenase activity of Herbaspirillum seropedicae grown under low iron levels requires the products of nifXorf1 genes.

    abstract::Herbaspirillum seropedicae strains mutated in the nifX or orf1 genes showed 90% or 50% reduction in nitrogenase activity under low levels of iron or molybdenum respectively. Mutations in nifX or orf1 genes did not affect nif gene expression since a nifH::lacZ fusion was fully active in both mutants. nifX and the conti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Klassen G,de Oliveira Pedrosa F,de Souza EM,Yates MG,Rigo LU

    更新日期:2003-07-29 00:00:00

  • RNA recovery and detection of mRNA by RT-PCR from preserved prokaryotic samples.

    abstract::The effectiveness of maintaining prokaryotic RNA in Synechococcus and Pseudomonas cells, fixed in 96% ethanol, 4% paraformaldehyde, or suspended in RNAlater, and held in cold storage for 3 months was compared. Fluorometric determination of the RNA extracted from Synechococcus and Pseudomonas cells indicated that the c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Bachoon DS,Chen F,Hodson RE

    更新日期:2001-07-24 00:00:00

  • Mutational analysis of the role of the conserved lysine-270 in the Pichia stipitis xylose reductase.

    abstract::Xylose reductase catalyzes the NAD(P)H-dependent reduction of xylose to xylitol and is essential for growth on xylose by yeasts. To understand the nature of coenzyme binding to the Pichia stipitis xylose reductase, we investigated the role of the strictly conserved Lys270 in the putative IPKS coenzyme binding motif by...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kostrzynska M,Sopher CR,Lee H

    更新日期:1998-02-01 00:00:00

  • Construction of a fusion protein carrying antigenic determinants of enteric clostridial toxins.

    abstract::Clostridium difficile and Clostridium perfringens type A are infectious agents of enteric diseases. The main virulence factors of these microorganisms include toxins A and B of C. difficile (ToxA and ToxB) and enterotoxin of C. perfringens (Cpe). In this study genetic constructions have been created for the expression...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Belyi IF,Varfolomeeva NA

    更新日期:2003-08-29 00:00:00

  • Taxonomic status of putative Verticillum alboatrum isolates.

    abstract::Two fungal isolates, formerly classified as Verticillium alboatrum and proposed as forming the basis of a new sub-group ('Group 2') within the species, have been shown to be non-pathogenic to known hosts of V. alboatrum and, on the basis of molecular evidence, to be closely related to Verticillium psalliotae and Verti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Carder JH,Barbara DJ

    更新日期:1999-01-01 00:00:00

  • Properties of the periplasmic nitrate reductases from Paracoccus pantotrophus and Escherichia coli after growth in tungsten-supplemented media.

    abstract::Paracoccus pantotrophus grown anaerobically under denitrifying conditions expressed similar levels of the periplasmic nitrate reductase (NAP) when cultured in molybdate- or tungstate-containing media. A native PAGE gel stained for nitrate reductase activity revealed that only NapA from molybdate-grown cells displayed ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gates AJ,Hughes RO,Sharp SR,Millington PD,Nilavongse A,Cole JA,Leach ER,Jepson B,Richardson DJ,Butler CS

    更新日期:2003-03-28 00:00:00

  • A fungus-type beta-galactofuranan in the cultivated Trebouxia photobiont of the lichen Ramalina gracilis.

    abstract::A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cordeiro LM,Carbonero ER,Sassaki GL,Reis RA,Stocker-Wörgötter E,Gorin PA,Iacomini M

    更新日期:2005-03-01 00:00:00

  • Mutational, proteomic and metabolomic analysis of a plant growth promoting copper-resistant Pseudomonas spp.

    abstract::Pseudomonas sp. TLC6-6.5-4 is a multiple metal resistant plant growth-promoting bacteria isolated from copper-contaminated lake sediments. In this study, a comprehensive analysis of genes involved in copper resistance was performed by generating a library of transposon (Tn5) mutants. Two copper-sensitive mutants with ...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Li K,Pidatala RR,Ramakrishna W

    更新日期:2012-10-01 00:00:00

  • Construction and properties of pyruvate dehydrogenase complexes with up to nine lipoyl domains per lipoate acetyltransferase chain.

    abstract::The lipoate acetyltransferase (E2p) subunits of the pyruvate dehydrogenase (PDH) complex of Escherichia coli have three tandemly repeated lipoyl domains, although net deletions of one or two has no apparent effect on the activity of the purified complexes. Plasmids containing IPTG-inducible aceEF-lpd operons, which en...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Machado RS,Clark DP,Guest JR

    更新日期:1992-12-15 00:00:00

  • High-frequency occurrence of virus-like particles with double-stranded RNA genome in Fusarium poae.

    abstract::Fifty-five geographically different strains of Fusarium poae were assayed for the presence of extrachromosomal nucleic acid elements. All strains were found to harbour double-stranded RNA (dsRNA) elements and encapsidated virus-like particles (VLP). There were great individual differences in dsRNA patterns of the vari...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Fekete C,Giczey G,Papp I,Szabó L,Hornok L

    更新日期:1995-09-15 00:00:00