Metabolic engineering of Escherichia coli for the production of isoprenoids.

abstract：:In Bordetella pertussis virulence-associated genes, including adenylate cyclase toxin (Cya), are coordinately regulated in response to environmental signals by proteins coded by the bvg-locus. We have constructed cya-lac fusions in Escherichia coli and have shown that the cya operon is not expressed in E. coli, neithe...

journal_title：FEMS microbiology letters

authors： Goyard S,Ullmann A

更新日期：1991-01-15 00:00:00

abstract：:The prodrug activation system formed by the E. coli codA gene encoding cytosine deaminase (CD) and 5-fluorocytosine (5-FC) developed for selective cancer chemotherapy suffers from a sensitivity limitation in many tumour cells. In an attempt to improve the CD/5-FC suicide association, we combined the E. coli upp gene e...

journal_title：FEMS microbiology letters

authors： Tiraby M,Cazaux C,Baron M,Drocourt D,Reynes JP,Tiraby G

更新日期：1998-10-01 00:00:00

abstract：:A gene encoding a phosphomannose isomerase from Streptococcus mutans GS-5 was identified immediately downstream from the fructokinase gene, scrK. Nucleotide sequence analysis of this region revealed an open reading frame (ORF) specifying a putative protein of 316 amino acids. The gene cloned in Escherichia coli expres...

journal_title：FEMS microbiology letters

authors： Sato Y,Yamamoto Y,Kizaki H,Kuramitsu HK

更新日期：1993-11-15 00:00:00

abstract：:In this study, we analysed the diversity and composition of double-stranded DNA viral and bacterial communities within the sample of surface coastal water of Southern Baikal through metagenomics and deep sequencing of the 16S ribosomal RNA gene, respectively. The 16S rRNA gene analysis has revealed 14 phyla and domina...

journal_title：FEMS microbiology letters

authors： Butina TV,Bukin YS,Krasnopeev AS,Belykh OI,Tupikin AE,Kabilov MR,Sakirko МV,Belikov SI

更新日期：2019-05-01 00:00:00

abstract：:The precise molecular mechanism of Staphylococcus aureus beta-toxin inactivation by the serotype F triple-converting phage phi 42, phi A1 and phi A3 was investigated. Sequence analysis of the phi 42 (attP) and Staphylococcus aureus (attB) attachment sites and the left (attL) and right (attR) chromosomal/bacteriophage ...

journal_title：FEMS microbiology letters

authors： Carroll JD,Cafferkey MT,Coleman DC

更新日期：1993-01-15 00:00:00

abstract：:Herbaspirillum seropedicae strains mutated in the nifX or orf1 genes showed 90% or 50% reduction in nitrogenase activity under low levels of iron or molybdenum respectively. Mutations in nifX or orf1 genes did not affect nif gene expression since a nifH::lacZ fusion was fully active in both mutants. nifX and the conti...

journal_title：FEMS microbiology letters

authors： Klassen G,de Oliveira Pedrosa F,de Souza EM,Yates MG,Rigo LU

更新日期：2003-07-29 00:00:00

abstract：:The distribution of the alcohol dehydrogenase gene (adh) among different Archaea was investigated by Southern blot analysis revealing the potentiality of the adh gene as a specific marker for the genus Sulfolobus. Moreover, the in vivo expression of the adh gene from a new isolate of Sulfolobus solfataricus, G theta, ...

journal_title：FEMS microbiology letters

authors： Cannio R,Fiorentino G,Rossi M,Bartolucci S

更新日期：1999-01-01 00:00:00

abstract：:Acetone-butanol-ethanol (ABE) fermentation is a metabolic process of clostridia that produces bio-based solvents including butanol. It is enabled by an underlying metabolic reaction network and modulated by cellular gene regulation and environmental cues. Mathematical modeling has served as a valuable strategy to faci...

journal_title：FEMS microbiology letters

authors： Liao C,Seo SO,Lu T

更新日期：2016-05-01 00:00:00

abstract：:A Tn5 transposon mutant was isolated of the alkylphenol degrader Pseudomonas sp. KL28 that showed increased growth at higher levels of m-cresol on solid and in liquid cultures. The transposon was inserted at the 5'-terminus of rpoS, which encodes a stationary-phase sigma factor. When grown on agar plates, the rpoS mut...

journal_title：FEMS microbiology letters

authors： Yun JI,Cho KM,Kim JK,Lee SO,Cho K,Lee K

更新日期：2007-04-01 00:00:00

abstract：:A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

journal_title：FEMS microbiology letters

authors： Shah DH,Verma R,Bakshi CS,Singh RK

更新日期：2002-08-27 00:00:00

abstract：:A protein kinase from Dictyostelium discoideum which phosphorylates the synthetic peptide, calmodulin-dependent protein kinase substrate (CDPKS, amino acid sequence: PLRRTLSVAA) and is stimulated by Ca2+/calmodulin is described. This is the first report of a protein kinase with these characteristics in D. discoideum. ...

journal_title：FEMS microbiology letters

authors： Dunbar AJ,Wheldrake JF

更新日期：1994-01-01 00:00:00

abstract：:An obligately anaerobic spirochete designated strain SEBR 4228T (T = type strain) was isolated from an oil field of Congo, Central Africa. The strain grew optimally with a sodium chloride concentration of 5% (sodium chloride concentration) growth range 1.0-10%) at 37 degrees C (growth temperature range 20-40 degrees C...

journal_title：FEMS microbiology letters

authors： Magot M,Fardeau ML,Arnauld O,Lanau C,Ollivier B,Thomas P,Patel BK

更新日期：1997-10-15 00:00:00

abstract：:The phylogenetic position of an acidophilic chemo-organotrophic menaquinone-containing bacterium, Acidobacterium capsulatum, was studied on the basis of 16S rRNA gene sequence information. A. capsulatum showed the highest level of sequence similarity to Heliobacterium chlorum, a member of the Gram-positive group, yet ...

journal_title：FEMS microbiology letters

authors： Hiraishi A,Kishimoto N,Kosako Y,Wakao N,Tano T

更新日期：1995-10-01 00:00:00

abstract：:Leishmania (Leishmania) infantum is the causative agent of both the cutaneous and visceral forms of leishmaniasis in southwest Europe; the dog is the main reservoir. In order to identify the L. (L.) infantum zymodemes present in Spain, a total number of 85 Leishmania stocks isolated from dogs (31), HIV-positive patien...

journal_title：FEMS microbiology letters

authors： Jiménez M,Ferrer-Dufol M,Cañavate C,Gutiérrez-Solar B,Molina R,Laguna F,López-Vélez R,Cercenado E,Daudén E,Blázquez J

更新日期：1995-09-01 00:00:00

abstract：:Mild alkaline solutions (beta-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans, respectively. When the beta-elimination was carried out after digestion of the walls with chitinase, four-fo...

journal_title：FEMS microbiology letters

authors： Mormeneo S,Marcilla A,Iranzo M,Sentandreu R

更新日期：1994-10-15 00:00:00

abstract：:Biotype, genome, protein and plasmid profile diversity amongst 40 epidemiologically unrelated strains of Helicobacter pylori was studied. Strains were API Zym biotypes II, III and IV but most (87%) were biotype II. Four subsets of strains were defined on a combination of motility (56% positive) and cytotoxin productio...

journal_title：FEMS microbiology letters

authors： Owen RJ,Bickley J,Moreno M,Costas M,Morgan DR

更新日期：1991-04-15 00:00:00

abstract：:In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

journal_title：FEMS microbiology letters

authors： Wolters M,Borst A,Pfeiffer F,Soppa J

更新日期：2019-11-01 00:00:00

abstract：:Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to p...

journal_title：FEMS microbiology letters

authors： Powell NG,Threlfall EJ,Chart H,Rowe B

更新日期：1994-06-01 00:00:00

abstract：:Enterococcal surface protein (Esp) is a cell wall-associated protein of Enterococcus faecalis that has been identified as a potential virulence factor. We used a mouse model to examine whether Esp facilitates intestinal colonization or translocation of E. faecalis to mesenteric lymph nodes. After clindamycin treatment...

journal_title：FEMS microbiology letters

authors： Pultz NJ,Shankar N,Baghdayan AS,Donskey CJ

更新日期：2005-01-15 00:00:00

abstract：:A high-level expression system for the Escherichia coli HB101 gamma-glutamyltranspeptidase (GGT) gene was constructed to obtain a sufficient amount of the enzyme for structural studies. About 300 mg of the enzyme was purified from 2 1 of culture broth. Both subunits were needed to reconstitute GGT activity. The E. col...

journal_title：FEMS microbiology letters

authors： Ishiye M,Niwa M

更新日期：1992-10-15 00:00:00

abstract：:DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

journal_title：FEMS microbiology letters

authors： Gowher H,Ehrlich KC,Jeltsch A

更新日期：2001-11-27 00:00:00

abstract：:The small heat shock protein (smHsp) Lo18 from lactic acid bacteria Oenococcus oeni reduces in vitro thermal aggregation of proteins and modulates the membrane fluidity of native liposomes. An absence of information relating to the way in which the smHsp demonstrates a stabilizing effect for both proteins and membrane...

journal_title：FEMS microbiology letters

authors： Weidmann S,Rieu A,Rega M,Coucheney F,Guzzo J

更新日期：2010-08-01 00:00:00

abstract：:It is often taken for granted that all animals host and depend upon a microbiome, yet this has only been shown for a small proportion of species. We propose that animals span a continuum of reliance on microbial symbionts. At one end are the famously symbiont-dependent species such as aphids, humans, corals and cows, ...

journal_title：FEMS microbiology letters

authors： Hammer TJ,Sanders JG,Fierer N

更新日期：2019-05-01 00:00:00

abstract：:Agrobacterium tumefaciens is the causal agent of crown gall disease and is a vector for DNA transfer in transgenic plants. The transformation process by A. tumefaciens has been widely studied, but the attachment stage has not been well characterized. Most measurements of attachment have used microscopy and colony coun...

journal_title：FEMS microbiology letters

authors： Petrovicheva A,Joyner J,Muth TR

更新日期：2017-10-02 00:00:00

abstract：:The transcriptional repressor Rex has been implicated in the regulation of energy metabolism and fermentative growth in response to redox potential. Streptococcus mutans, the primary causative agent of human dental caries, possesses a gene that encodes a protein with high similarity to members of the Rex family of pro...

journal_title：FEMS microbiology letters

authors： Bitoun JP,Nguyen AH,Fan Y,Burne RA,Wen ZT

更新日期：2011-07-01 00:00:00

abstract：:Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

journal_title：FEMS microbiology letters

authors： Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

更新日期：2004-09-15 00:00:00

abstract：:The outer membrane proteins of several prominent bacterial pathogens demonstrate substantial variation in their surface antigenic epitopes. To determine if this was also true for Pseudomonas aeruginosa outer membrane protein OprF, gene sequencing of a serotype 5 isolate was performed to permit comparison with the publ...

journal_title：FEMS microbiology letters

authors： Martin NL,Rawling EG,Wong RS,Rosok M,Hancock RE

更新日期：1993-11-01 00:00:00

abstract：:Some microorganisms found in the soil are able to produce substances which regulate plant growth. In this study, we show the presence of a substance associated with auxin activity, identified as indole-3-butyric acid (IBA), in Azospirillum brasilense UAP 154 growth medium. A. brasilense was grown and indolic compounds...

journal_title：FEMS microbiology letters

authors： Martínez-Morales LJ,Soto-Urzúa L,Baca BE,Sánchez-Ahédo JA

更新日期：2003-11-21 00:00:00

abstract：:The est gene encoding an esterase from Acinetobacter lwoffii RAG-1 was cloned into E. coli under the control of the PL promoter of the phage lambda. The N-terminal sequence of the first 20 amino acids of the heterologous expressed esterase corresponded to that obtained from the nucleotide sequence. Antibodies prepared...

journal_title：FEMS microbiology letters

authors： Alon RN,Gutnick DL

更新日期：1993-09-15 00:00:00

abstract：:Four distinct bands of cysteine proteinase activity were detected when stationary-phase populations of Leishmania mexicana mexicana were subjected to gelatin-SDS-PAGE. The highest mobility band contained at least three isoforms separable by mono Q anion exchange chromatography. These high mobility activities were dist...

journal_title：FEMS microbiology letters

authors： Robertson CD,Coombs GH

更新日期：1992-07-01 00:00:00