Abstract:
:Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant clinical isolate. Here, we have evaluated the expression of these genes in 24 clinical isolates that belong to different lineages and have different drug resistance profiles. In vitro, growth kinetics analysis showed no difference in the growth of the clinical isolates, and thus drug resistance occurred without a fitness cost. However, a quantitative reverse transcription PCR analysis of gene expression revealed high variability among the clinical isolates, including those with similar drug resistance profiles. Due to the complexity of gene regulation pathways and the wide diversity of M. tuberculosis lineages, the use of gene expression as a molecular signature for drug resistance is not straightforward. Therefore, we recommend that the expression of M. tuberculosis genes be performed individually, and baseline expression levels should be verified among several different clinical isolates, before any further applications of these findings.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
González-Escalante L,Peñuelas-Urquides K,Said-Fernández S,Silva-Ramírez B,Bermúdez de León Mdoi
10.1093/femsle/fnv194subject
Has Abstractpub_date
2015-12-01 00:00:00pages
fnv194issue
23eissn
0378-1097issn
1574-6968pii
fnv194journal_volume
362pub_type
杂志文章abstract::We have previously reported that mycolyl glycolipids from Nocardia rubra such as glucose or trehalose mycolates induced granuloma formation in mice. The structure of the carbohydrate moiety of the mycolyl glycolipids influenced the granuloma forming activity profoundly. Here, we have examined the macrophage-chemotacti...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(90)90166-n
更新日期:1990-01-15 00:00:00
abstract::An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop a...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2011.02376.x
更新日期:2011-10-01 00:00:00
abstract::The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. franç...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00526-3
更新日期:2003-09-12 00:00:00
abstract::Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/s0378-1097(05)80049-7
更新日期:1990-03-15 00:00:00
abstract::There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00529-9
更新日期:2003-08-29 00:00:00
abstract::Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2007.00772.x
更新日期:2007-08-01 00:00:00
abstract::The aecA and aecB loci map at 250 and 290 degrees, respectively, on the Bacillus subtilis chromosomal genetic map. The aecB locus has been proposed as the structural gene for aspartokinase II. From DNA sequence analyses and comparisons to the sequence of the aspartokinase II gene, it can be concluded that the structur...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(89)90175-4
更新日期:1989-10-01 00:00:00
abstract::Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2001.tb10809.x
更新日期:2001-08-21 00:00:00
abstract::This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all mark...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/j.femsle.2004.07.034
更新日期:2004-09-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章,评审
doi:10.1111/j.1574-6968.1998.tb12792.x
更新日期:1998-01-01 00:00:00
abstract::Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sam...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2006.00217.x
更新日期:2006-05-01 00:00:00
abstract::The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnz132
更新日期:2019-06-01 00:00:00
abstract::Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (FADB::...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2002.tb11247.x
更新日期:2002-06-18 00:00:00
abstract::Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic too...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/j.femsle.2005.09.021
更新日期:2005-12-01 00:00:00
abstract::A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1993.tb06310.x
更新日期:1993-06-15 00:00:00
abstract::The interaction of Entamoeba histolytica trophozoites with collagen type I and calcium induces a membrane to nuclei signaling. The transduction pathways involved in such phenomena are still poorly understood. Using a combination of immunoprecipitation assays, Western immunoblot analysis, electrophoretic mobility shift...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00822-X
更新日期:2003-12-12 00:00:00
abstract::Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/S0378-1097(03)00916-9
更新日期:2004-01-30 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/1574-6968.12626
更新日期:2014-12-01 00:00:00
abstract::Sphingobacterium sp. RB, a novel bacterial strain isolated from a soil sample, was able to utilize para-nitrophenol (PNP) as sole source of carbon and energy at high concentrations (1.0-5.0 mM). The culture completely degraded 3.0 mM PNP within 36 h with proportionate increase in biomass. With 5.0 mM PNP (700 ppm), 70...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1093/femsle/fnz168
更新日期:2019-07-01 00:00:00
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2012.02543.x
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journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1997.tb10291.x
更新日期:1997-03-15 00:00:00
abstract::We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/0378-1097(94)90251-8
更新日期:1994-12-01 00:00:00
abstract::Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine fece...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2000.tb08919.x
更新日期:2000-01-15 00:00:00
abstract::The pFL10 plasmid vector for translational fusions was constructed. pFL10 is based in the promiscuous pLS1 replicon and contains the pC194 cat gene deprived of its transcriptional promoter and Shine-Dalgarno (SD) sequence. Three promoters (Pcit, PpolA and PtetL) from Gram-positive bacteria, inserted in pFL10, were tes...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1994.tb07182.x
更新日期:1994-10-01 00:00:00
abstract::It is often claimed that Antoni van Leeuwenhoek was secretive about his methods. However, closer examination of his letters suggests that this reputation was not always deserved. Some letters were not published in the Royal Society Proceedings, and others were edited. This paper describes the repetition of his experim...
journal_title:FEMS microbiology letters
pub_type: 历史文章,杂志文章
doi:10.1093/femsle/fnz194
更新日期:2019-08-01 00:00:00
abstract::Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competi...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1996.tb08559.x
更新日期:1996-11-15 00:00:00
abstract::Previous studies had shown that Mycoplasma pulmonis contained a bovine serum albumin-dependent, membrane-associated hemolysin. Biochemical analyses were performed to further characterize this activity. The membrane-associated hemolytic activity could be activated by dithiothreitol and beta-mercaptoethanol, and inactiv...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.1995.tb07525.x
更新日期:1995-05-01 00:00:00
abstract::Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1016/j.femsle.2004.07.058
更新日期:2004-09-15 00:00:00
abstract::Phlebia tremellosa decolourised eight synthetic textile dyes (200 mg l(-1)) by greater than 96% within 14 days under stationary incubation conditions. High performance liquid chromatography analysis of culture supernatants indicated that Remazol Black B was degraded by the fungus, however, complete mineralisation did ...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2000.tb09174.x
更新日期:2000-07-01 00:00:00
abstract::Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The ai...
journal_title:FEMS microbiology letters
pub_type: 杂志文章
doi:10.1111/j.1574-6968.2009.01670.x
更新日期:2009-08-01 00:00:00