Differential expression of putative drug resistance genes in Mycobacterium tuberculosis clinical isolates.

Abstract:

:Understanding drug resistance in Mycobacterium tuberculosis requires an integrated analysis of strain lineages, mutations and gene expression. Previously, we reported the differential expression of esxG, esxH, infA, groES, rpmI, rpsA and lipF genes in a sensitive M. tuberculosis strain and in a multidrug-resistant clinical isolate. Here, we have evaluated the expression of these genes in 24 clinical isolates that belong to different lineages and have different drug resistance profiles. In vitro, growth kinetics analysis showed no difference in the growth of the clinical isolates, and thus drug resistance occurred without a fitness cost. However, a quantitative reverse transcription PCR analysis of gene expression revealed high variability among the clinical isolates, including those with similar drug resistance profiles. Due to the complexity of gene regulation pathways and the wide diversity of M. tuberculosis lineages, the use of gene expression as a molecular signature for drug resistance is not straightforward. Therefore, we recommend that the expression of M. tuberculosis genes be performed individually, and baseline expression levels should be verified among several different clinical isolates, before any further applications of these findings.

journal_name

FEMS Microbiol Lett

authors

González-Escalante L,Peñuelas-Urquides K,Said-Fernández S,Silva-Ramírez B,Bermúdez de León M

doi

10.1093/femsle/fnv194

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

fnv194

issue

23

eissn

0378-1097

issn

1574-6968

pii

fnv194

journal_volume

362

pub_type

杂志文章
  • Mycolyl glycolipids stimulate macrophages to release a chemotactic factor.

    abstract::We have previously reported that mycolyl glycolipids from Nocardia rubra such as glucose or trehalose mycolates induced granuloma formation in mice. The structure of the carbohydrate moiety of the mycolyl glycolipids influenced the granuloma forming activity profoundly. Here, we have examined the macrophage-chemotacti...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90166-n

    authors: Matsunaga I,Oka S,Inoue T,Yano I

    更新日期:1990-01-15 00:00:00

  • Isolation of the fenoxaprop-ethyl (FE)-degrading bacterium Rhodococcus sp. T1, and cloning of FE hydrolase gene feh.

    abstract::An enrichment culture which completely degraded fenoxaprop-ethyl (FE) was acquired by using FE as sole carbon source. An efficient FE-degrading strain T1 was isolated from the enrichment culture and identified as Rhodococcus sp. Strain T1 could degrade 94% of 100 mg L(-1) FE within 24 h and the metabolite fenoxaprop a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2011.02376.x

    authors: Hou Y,Tao J,Shen W,Liu J,Li J,Li Y,Cao H,Cui Z

    更新日期:2011-10-01 00:00:00

  • Effect of Urtica dioica agglutinin and Arabidopsis thaliana Chia4 chitinase on the protozoan Phytomonas françai.

    abstract::The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. franç...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00526-3

    authors: Gomes Rocha GC,Nicolich R,Romeiro A,Margis-Pinheiro M,Attias M,Alves-Ferreira M

    更新日期:2003-09-12 00:00:00

  • Adherence of Candida albicans to components of the subendothelial extracellular matrix.

    abstract::Candida albicans yeasts adhered avidly to extracellular matrix (ECM) proteins, type IV collagen, laminin, and fibronectin immobilized on plastic. Type IV collagen showed an increase of adherence of 400% above control values; laminin, 300%; and fibronectin, 150%. In addition, all three (in quantities of 0.02-200 microg...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(05)80049-7

    authors: Klotz SA

    更新日期:1990-03-15 00:00:00

  • Genetic evidence for the existence of two pathways for the biosynthesis of methionine in the Leptospira spp.

    abstract::There are two major pathways for methionine biosynthesis: the enterobacterial type transsulfuration pathway and the sulfhydrylation pathway as previously identified in the spirochete Leptospira meyeri. Sequence analysis of the L. meyeri metYX locus allows the identification of a third gene, called metW, which encodes ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00529-9

    authors: Picardeau M,Bauby H,Saint Girons I

    更新日期:2003-08-29 00:00:00

  • Sesquiterpene farnesol as a competitive inhibitor of lipase activity of Staphylococcus aureus.

    abstract::Staphylococcus aureus lipase (SAL) is known to possess broad substrate specificity for triacylglycerides. We found that a sub-minimum inhibitory concentration of farnesol (1000 mg L(-1)) inhibits this lipase activity on a Mueller-Hinton agar containing 1% Tween substrates. A quantitative lipase assay using p-nitrophen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2007.00772.x

    authors: Kuroda M,Nagasaki S,Ito R,Ohta T

    更新日期:2007-08-01 00:00:00

  • The structural gene for aspartokinase II in Bacillus subtilis is closely linked to the sdh operon.

    abstract::The aecA and aecB loci map at 250 and 290 degrees, respectively, on the Bacillus subtilis chromosomal genetic map. The aecB locus has been proposed as the structural gene for aspartokinase II. From DNA sequence analyses and comparisons to the sequence of the aspartokinase II gene, it can be concluded that the structur...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(89)90175-4

    authors: Petricek M,Rutberg L,Hederstedt L

    更新日期:1989-10-01 00:00:00

  • Identification of a secreted superoxide dismutase in Mycobacterium avium ssp. paratuberculosis.

    abstract::Mycobacterium avium ssp. paratuberculosis (M. paratuberculosis), the causative agent of Johne's disease, is an important animal pathogen that has also been implicated in human disease. The major proteins expressed by M. paratuberculosis were analyzed by two-dimensional gel electrophoresis, and a superoxide dismutase (...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10809.x

    authors: Liu X,Feng Z,Harris NB,Cirillo JD,Bercovier H,Barletta RG

    更新日期:2001-08-21 00:00:00

  • Characterization of SCAR markers of Eimeria spp. of domestic fowl and construction of a public relational database (The Eimeria SCARdb).

    abstract::This study reports the development and characterization of 151 sequence characterized amplified region (SCAR) markers for the seven Eimeria species that infect the domestic fowl. From this set, 84 markers are species-specific and 67 present partial specificity. The complete nucleotide sequence was derived for all mark...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.034

    authors: Fernandez S,Katsuyama AM,Kashiwabara AY,Madeira AM,Durham AM,Gruber A

    更新日期:2004-09-01 00:00:00

  • ABC transporters in antibiotic-producing actinomycetes.

    abstract::Many antibiotic-producing actinomycetes possess at least one ABC (ATP-binding cassette) transporter which forms part of the antibiotic biosynthetic pathway and in most cases confers resistance to the drug in an heterologous host. Three types of antibiotic ABC transporters have been so far described in producer organis...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章,评审

    doi:10.1111/j.1574-6968.1998.tb12792.x

    authors: Méndez C,Salas JA

    更新日期:1998-01-01 00:00:00

  • Isolation and characterization of bacteriophages infecting Salmonella spp.

    abstract::Bacteriophages infecting Salmonella spp. were isolated from sewage using soft agar overlays containing three Salmonella serovars and assessed with regard to their potential to control food-borne salmonellae. Two distinct phages, as defined by plaque morphology, structure and host range, were obtained from a single sam...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00217.x

    authors: Carey-Smith GV,Billington C,Cornelius AJ,Hudson JA,Heinemann JA

    更新日期:2006-05-01 00:00:00

  • The impact factor: implications for research policy, editorial rules and scholarly reputation.

    abstract::The assessment of scientific research is essentially based on several metric parameters, the so-termed Impact Factor perhaps being the predominant one. Despite well-founded criticisms and the wide opposition of reputed scientists, this procedure has become a tool of scientific policy, and is applied in editorial proce...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz132

    authors: Argüelles JC,Argüelles-Prieto R

    更新日期:2019-06-01 00:00:00

  • Polyhydroxyalkanoate biosynthesis in Pseudomonas pseudoalcaligenes YS1.

    abstract::Pseudomonas pseudoalcaligenes strain YS1 isolated from oil contaminated soil was able to produce polyhydroxybutyrate blended with medium-chain-length polyhydroxyalkanoates (mcl PHA). PHA synthesis genes were cloned from this strain. A fadB (gene for fatty acid degradation) deleted mutant Escherichia coli KM32B (FADB::...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2002.tb11247.x

    authors: Hang X,Lin Z,Chen J,Wang G,Hong K,Chen GQ

    更新日期:2002-06-18 00:00:00

  • Construction of the first shuttle vectors for gene cloning and homologous recombination in Mycoplasma agalactiae.

    abstract::Mycoplasma agalactiae is a worldwide ruminant pathogen that causes significant economic losses by inflicting contagious agalactia in sheep and goats. The development of efficient control strategies requires a better understanding of the mycoplasma factors that promote successful infection. However, lack of genetic too...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.09.021

    authors: Chopra-Dewasthaly R,Marenda M,Rosengarten R,Jechlinger W,Citti C

    更新日期:2005-12-01 00:00:00

  • Neisseria meningitidis transferrin-binding protein 1 expressed in Escherichia coli is surface exposed and binds human transferrin.

    abstract::A gene library of Neisseria meningitidis B15 P1.16 DNA was established in lambda Zap II and clones containing DNA encoding transferrin binding protein 1 (TBP-1) identified following hybridisation with a 63-bp DNA probe based on the codon assignment for the first 21 N-terminal amino acids of TBP-1. Sequencing of the cl...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1993.tb06310.x

    authors: Palmer HM,Powell NB,Ala'Aldeen DA,Wilton J,Borriello SP

    更新日期:1993-06-15 00:00:00

  • Collagen-induced STAT family members activation in Entamoeba histolytica trophozoites.

    abstract::The interaction of Entamoeba histolytica trophozoites with collagen type I and calcium induces a membrane to nuclei signaling. The transduction pathways involved in such phenomena are still poorly understood. Using a combination of immunoprecipitation assays, Western immunoblot analysis, electrophoretic mobility shift...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00822-X

    authors: Cruz-Vera J,Clara L,Hernández-Kelly R,Alfredo Méndez J,Pérez-Salazar E,Ortega A

    更新日期:2003-12-12 00:00:00

  • Characterization of the protein processing and secretion pathways in a comprehensive set of expressed sequence tags from Trichoderma reesei.

    abstract::Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/S0378-1097(03)00916-9

    authors: Diener SE,Dunn-Coleman N,Foreman P,Houfek TD,Teunissen PJ,van Solingen P,Dankmeyer L,Mitchell TK,Ward M,Dean RA

    更新日期:2004-01-30 00:00:00

  • The Listeria monocytogenes transposon Tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide.

    abstract::Tolerance of the foodborne pathogen Listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. Particularly, quaternary ammonium compounds (QACs) such as benzalkonium chloride (BC) are often used in disinfectants and also as antiseptics in food industry and hospitals. Recently, w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12626

    authors: Müller A,Rychli K,Zaiser A,Wieser C,Wagner M,Schmitz-Esser S

    更新日期:2014-12-01 00:00:00

  • A novel Sphingobacterium sp. RB, a rhizosphere isolate degrading para-nitrophenol with substrate specificity towards nitrotoluenes and nitroanilines.

    abstract::Sphingobacterium sp. RB, a novel bacterial strain isolated from a soil sample, was able to utilize para-nitrophenol (PNP) as sole source of carbon and energy at high concentrations (1.0-5.0 mM). The culture completely degraded 3.0 mM PNP within 36 h with proportionate increase in biomass. With 5.0 mM PNP (700 ppm), 70...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz168

    authors: Samson R,Bodade R,Zinjarde S,Kutty R

    更新日期:2019-07-01 00:00:00

  • Facilitated recruitment of Pdc2p, a yeast transcriptional activator, in response to thiamin starvation.

    abstract::In Saccharomyces cerevisiae, genes involved in thiamin pyrophosphate (TPP) synthesis (THI genes) and the pyruvate decarboxylase structural gene PDC5 are transcriptionally induced in response to thiamin starvation. Three positive regulatory factors (Thi2p, Thi3p, and Pdc2p) are involved in the expression of THI genes, ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2012.02543.x

    authors: Nosaka K,Esaki H,Onozuka M,Konno H,Hattori Y,Akaji K

    更新日期:2012-05-01 00:00:00

  • Glucose stimulates alginate production and algD transcription in Pseudomonas aeruginosa.

    abstract::A previous study [DeVault et al. (1991) Mol. Microbiol. 5, 2503-2509] suggested that growth of Pseudomonas aeruginosa in glucose-containing medium represses algD gene transcription. In this study, growth of P. aeruginosa in rich medium containing glucose or gluconate increased alginate production and algD transcriptio...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1997.tb10291.x

    authors: Ma JF,Phibbs PV,Hassett DJ

    更新日期:1997-03-15 00:00:00

  • Mutation in dipZ leads to reduced production of active human placental alkaline phosphatase in Escherichia coli.

    abstract::We have tested the expression of alkaline phosphatases in a mutant strain of Escherichia coli deficient in dipZ, a gene coding for a protein involved in cytochrome c biogenesis, and the isogenic wild-type strain. The yield of soluble and active human placental alkaline phosphatase was significantly reduced in the muta...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(94)90251-8

    authors: Beck R,Crooke H,Jarsch M,Cole J,Burtscher H

    更新日期:1994-12-01 00:00:00

  • Isolation and characterization of Escherichia coli O157 from retail beef and bovine feces in Thailand.

    abstract::Antibody to Escherichia coli O157 lipopolysaccharide was detected in the sera of healthy individuals more frequently in Southern Thailand than in Japan. The result suggested possible exposure of Thai people to E. coli O157. E. coli O157:H7 or O157:H(-) was isolated from four of 95 retail beef and one of 55 bovine fece...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb08919.x

    authors: Vuddhakul V,Patararungrong N,Pungrasamee P,Jitsurong S,Morigaki T,Asai N,Nishibuchi M

    更新日期:2000-01-15 00:00:00

  • Comparative analysis of gene expression in Streptococcus pneumoniae and Lactococcus lactis.

    abstract::The pFL10 plasmid vector for translational fusions was constructed. pFL10 is based in the promiscuous pLS1 replicon and contains the pC194 cat gene deprived of its transcriptional promoter and Shine-Dalgarno (SD) sequence. Three promoters (Pcit, PpolA and PtetL) from Gram-positive bacteria, inserted in pFL10, were tes...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb07182.x

    authors: López de Felipe F,Corrales MA,López P

    更新日期:1994-10-01 00:00:00

  • Was Antoni van Leeuwenhoek secretive? His experiments with insect corneas.

    abstract::It is often claimed that Antoni van Leeuwenhoek was secretive about his methods. However, closer examination of his letters suggests that this reputation was not always deserved. Some letters were not published in the Royal Society Proceedings, and others were edited. This paper describes the repetition of his experim...

    journal_title:FEMS microbiology letters

    pub_type: 历史文章,杂志文章

    doi:10.1093/femsle/fnz194

    authors: Robertson LA

    更新日期:2019-08-01 00:00:00

  • Antigenicity of a viral peptide displayed on beta-galactosidase fusion proteins is influenced by the presence of the homologous partner protein.

    abstract::Several beta-galactosidase fusion proteins have been constructed containing the entire VP1 protein from foot-and-mouth disease virus (FMDV) [Corchero et al. (1996) J. Biotechnol. in press]. The antigenicity of the major immunodominant site A (13 amino acids in length) within the VP1 protein has been studied in competi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1996.tb08559.x

    authors: Corchero JL,Villaverde A

    更新日期:1996-11-15 00:00:00

  • The effect of thiol-active compounds and sterols on the membrane-associated hemolysin of Mycoplasma pulmonis.

    abstract::Previous studies had shown that Mycoplasma pulmonis contained a bovine serum albumin-dependent, membrane-associated hemolysin. Biochemical analyses were performed to further characterize this activity. The membrane-associated hemolytic activity could be activated by dithiothreitol and beta-mercaptoethanol, and inactiv...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07525.x

    authors: Jarvill-Taylor KJ,Minion FC

    更新日期:1995-05-01 00:00:00

  • Characterization of extended-spectrum-beta-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal.

    abstract::Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.058

    authors: Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

    更新日期:2004-09-15 00:00:00

  • Decolourisation of synthetic textile dyes by Phlebia tremellosa.

    abstract::Phlebia tremellosa decolourised eight synthetic textile dyes (200 mg l(-1)) by greater than 96% within 14 days under stationary incubation conditions. High performance liquid chromatography analysis of culture supernatants indicated that Remazol Black B was degraded by the fungus, however, complete mineralisation did ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09174.x

    authors: Kirby N,Marchant R,McMullan G

    更新日期:2000-07-01 00:00:00

  • Thiabendazole resistance and mutations in the beta-tubulin gene of Penicillium expansum strains isolated from apples and pears with blue mold decay.

    abstract::Penicillium expansum is the causal agent of blue mold rot, a postharvest decay of stored fruits. This fungus also produces the mycotoxins patulin and citrinin. Control of P. expansum still relies mainly on the use of fungicides such as thiabendazole. Since its introduction, resistant strains have been reported. The ai...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2009.01670.x

    authors: Cabañas R,Castellá G,Abarca ML,Bragulat MR,Cabañes FJ

    更新日期:2009-08-01 00:00:00