Abstract:
:Based on second generation sequencing (MiSeq platform, Illumina), we determined the genetic diversity of T4-like bacteriophages of the family Myoviridae by analysing fragments of the major capsid protein gene g23 in the plankton of Lake Baikal. The sampling depth in our study was significantly higher than in those obtained by the Sanger method before. We obtained 33 701 sequences of the g23 gene fragments, 141 operational taxonomic units (OTUs) of which were identified. 86 OTUs (60.9%) had the closest relatives from lakes Bourget and Annecy, and 28 OTUs (19.8%) had the highest identity with the Baikal g23 clones, which had been previously identified in the northern and southern basins of the lake by the Sanger method. The remaining OTUs were similar to the clones from other ecosystems. We showed a high genetic diversity of T4-type bacteriophages and a genetic difference with the phage communities from other ecosystems.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Potapov S,Belykh O,Krasnopeev A,Gladkikh A,Kabilov M,Tupikin A,Butina Tdoi
10.1093/femsle/fnx264subject
Has Abstractpub_date
2018-02-01 00:00:00issue
3eissn
0378-1097issn
1574-6968pii
4693836journal_volume
365pub_type
杂志文章abstract::We found the presence of DNA sequence which shows sequence similarity to the class IV chitin synthase gene (CHS3) of Saccharomyces cerevisiae in the genome of 14 Rhizopus species which belong to zygomycetes. We cloned a gene (chs3), which might correspond to one of these homologous sequences, from Rhizopus oligosporus...
journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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更新日期:1994-01-01 00:00:00
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