Characterization of two genes (hupD and hupE) required for hydrogenase activity in Azotobacter chroococcum.

abstract：:One hundred and ninety-eight clinical isolates of Mycobacterium kansasii collected between 2003 and 2004 in Japan were genotyped by PCR and restriction enzyme analysis (PRA) and 16S-23S internal transcribed spacer (ITS) sequencing. The results demonstrated that clinical isolates of M. kansasii in Japan are almost excl...

journal_title：FEMS microbiology letters

authors： Iwamoto T,Saito H

更新日期：2006-01-01 00:00:00

abstract：:The capsular polysaccharide released from the bacterial surface by cell wall turnover during growth exhibited less size heterogeneity and a higher average molecular mass than the polysaccharide extracted from the cell by treatment with lysostaphin or low pH. Treatment of turnover polysaccharide, radiolabelled by growt...

journal_title：FEMS microbiology letters

authors： Havaei SA,Hancock IC

更新日期：1994-05-01 00:00:00

abstract：:A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compou...

journal_title：FEMS microbiology letters

authors： Yamamoto S,Fujita Y,Okujo N,Minami C,Matsuura S,Shinoda S

更新日期：1992-07-01 00:00:00

abstract：:Two Pseudomonas aeruginosa genes, termed phaJ1(Pa) and phaJ2(Pa), homologous to the Aeromonas caviae (R)-specific enoyl-CoA hydratase gene (phaJ(Ac)) were cloned using a PCR technique to investigate the monomer-supplying ability for polyhydroxyalkanoate (PHA) synthesis from beta-oxidation cycle. Two expression plasmid...

journal_title：FEMS microbiology letters

authors： Tsuge T,Fukui T,Matsusaki H,Taguchi S,Kobayashi G,Ishizaki A,Doi Y

更新日期：2000-03-15 00:00:00

abstract：:Three tri-substituted spermidines, di-p-coumaroyl-caffeoylspermidine, tri-caffeoylspermidine and tri-p-coumaroylspermidine, isolated from pollen of Quercus alba, were examined for antifungal activity. Both di-p-coumaroyl-caffeoylspermidine and tri-p-coumaroylspermidine reduced mycelial growth of the oat leaf stripe pa...

journal_title：FEMS microbiology letters

authors： Walters D,Meurer-Grimes B,Rovira I

更新日期：2001-07-24 00:00:00

abstract：:The frequency of spontaneous as well as induced reversions of auxotrophic mutations in Escherichia coli AB1157 and its DeltadnaK and DeltadnaKdnaJ derivatives was estimated. The obtained results demonstrate that both mutants tested are characterized by elevated frequency of spontaneous reversions compared to their AB1...

journal_title：FEMS microbiology letters

authors： Grudniak AM,Kuć M,Wolska KI

更新日期：2005-01-15 00:00:00

abstract：:The twin-arginine translocation (Tat) system serves to translocate folded and often cofactor-containing proteins across biological membranes. The mechanistic limits of the Tat system can be explored by addressing the transport of specifically designed Tat substrates. It thus could be recently shown that unstructured p...

journal_title：FEMS microbiology letters

authors： Lindenstrauss U,Brüser T

更新日期：2009-06-01 00:00:00

abstract：:Thermophilic bacteria have recently attracted great attention because of their potential application in improving different biochemical processes such as anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA-targeted oligonu...

journal_title：FEMS microbiology letters

authors： Gagliano MC,Braguglia CM,Rossetti S

更新日期：2014-09-01 00:00:00

abstract：:Two thermostable phytases were identified from Thai isolates of Aspergillus japonicus BCC18313 (TR86) and Aspergillus niger BCC18081 (TR170). Both genes of 1404 bp length, coding for putative phytases of 468 amino acid residues, were cloned and transferred into Pichia pastoris. The recombinant phytases, r-PhyA86 and r...

journal_title：FEMS microbiology letters

authors： Promdonkoy P,Tang K,Sornlake W,Harnpicharnchai P,Kobayashi RS,Ruanglek V,Upathanpreecha T,Vesaratchavest M,Eurwilaichitr L,Tanapongpipat S

更新日期：2009-01-01 00:00:00

abstract：:Nucleoside diphosphate kinase (NDK) is a housekeeping enzyme localized in cellular organelles and distributed in various organs in prokaryotes and eukaryotes. In Neurospora crassa, NDK-1 is suggested to control catalases in response to heat, oxidative stress and light. In this study, we identified the presence of NDK-...

journal_title：FEMS microbiology letters

authors： Yoshida Y,Hasunuma K

更新日期：2006-08-01 00:00:00

abstract：:Nitrogen is a key nutrient for land plants and phytoplankton in terrestrial and aquatic ecosystems. The model alga Chlamydomonas reinhardtii can grow efficiently on several inorganic nitrogen sources (e.g. ammonium, nitrate, nitrite) as well as many amino acids. In this study, we show that Chlamydomonas is unable to u...

journal_title：FEMS microbiology letters

authors： Calatrava V,Hom EFY,Llamas Á,Fernández E,Galván A

更新日期：2018-04-01 00:00:00

abstract：:The glycolytic pathway of the hyperthermophilic archaea that belong to the order Thermococcales (Pyrococcus, Thermococcus and Palaeococcus) differs significantly from the canonical Embden-Meyerhof pathway in bacteria and eukarya. This archaeal glycolysis variant consists of several novel enzymes, some of which catalyz...

journal_title：FEMS microbiology letters

authors： van de Werken HJ,Verhees CH,Akerboom J,de Vos WM,van der Oost J

更新日期：2006-07-01 00:00:00

abstract：:Both ThyA and ThyX proteins catalyze the transfer of the methyl group from methylenetetrahydrofolate (CH(2) H(4) -folate) to dUMP, forming dTMP. To estimate the relative steady state expression levels of ThyA and ThyX, Western blot analysis was performed using ThyA or ThyX antiserum on total protein from the wild-type...

journal_title：FEMS microbiology letters

authors： Cho S,Yang S,Rhie H

更新日期：2012-03-01 00:00:00

abstract：:The gene nfxB is one of the genes which affect the cell membrane permeability of quinolones in Pseudomonas aeruginosa PAO. Both wild-type nfxB and a mutant nfxB (nfx13E) were cloned and the DNA sequences were determined. The wild-type gene was dominant in PAO strains. The nfxB mutation was a point mutation (cytosine--...

journal_title：FEMS microbiology letters

authors： Okazaki T,Hirai K

更新日期：1992-10-01 00:00:00

abstract：:Chemical and molecular taxonomic studies were performed on a representative strain of some lactic acid bacteria of unknown taxonomic position isolated from salmonid fish. The results demonstrate that the fish bacterium represents a new species of the genus Lactococcus for which the name Lactococcus piscium sp. nov. is...

journal_title：FEMS microbiology letters

authors： Williams AM,Fryer JL,Collins MD

更新日期：1990-03-01 00:00:00

abstract：:There are arguments against the conclusion drawn by Haselwandter and Ebner that fungal spores have survived for some 5300 years on hay padding in the leather boots of a frozen body discovered in the Austrian Alps. According to cryobiological experience, long-term survival of fungal spores is very unlikely at temperatu...

journal_title：FEMS microbiology letters

authors： Gams W,Stalpers JA

更新日期：1994-07-01 00:00:00

abstract：:In this study, the influence of the size and surface termination of diamond nanoparticles (DNPs) on their antibacterial activity against Escherichia coli and Bacillus subtilis was assessed. The average size and distribution of DNPs were determined by dynamic light scattering and X-ray diffraction techniques. The chemi...

journal_title：FEMS microbiology letters

authors： Beranová J,Seydlová G,Kozak H,Benada O,Fišer R,Artemenko A,Konopásek I,Kromka A

更新日期：2014-02-01 00:00:00

abstract：:DNA from Aspergillus sp. has been reported not to contain 5-methylcytosine. However, it has been found that Aspergillus nidulans responds to 5-azacytidine, a drug that is a strong inhibitor of DNA methyltransferases. Therefore, we have re-examined the occurrence of 5-methylcytosine in DNA from Aspergillus flavus by us...

journal_title：FEMS microbiology letters

authors： Gowher H,Ehrlich KC,Jeltsch A

更新日期：2001-11-27 00:00:00

abstract：:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) ...

journal_title：FEMS microbiology letters

authors： Edwards K,Linetsky I,Hueser C,Eisenstark A

更新日期：2001-05-30 00:00:00

abstract：:We present evidence that the anthrax toxin lethal factor binds multiple zinc atoms. Results from atomic adsorption spectroscopy indicate that lethal factor contains approximately three zinc atoms per toxin molecule. Lethal factor treated with EDTA and o-phenanthroline contained a similar number of zinc atoms, indicati...

journal_title：FEMS microbiology letters

authors： Kochi SK,Schiavo G,Mock M,Montecucco C

更新日期：1994-12-15 00:00:00

abstract：:A DNA-based method for the early detection and identification of decay basidiomycetes on wood chips is described. From 26 species of wood-decaying basidiomycetes and 20 species of wood-inhabiting ascomycetes, DNA fragments containing the internal transcribed spacers, 5.8S and partial 28S rDNA were amplified by PCR usi...

journal_title：FEMS microbiology letters

authors： Adair S,Kim SH,Breuil C

更新日期：2002-05-21 00:00:00

abstract：:A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, a...

journal_title：FEMS microbiology letters

authors： Cordeiro LM,Carbonero ER,Sassaki GL,Reis RA,Stocker-Wörgötter E,Gorin PA,Iacomini M

更新日期：2005-03-01 00:00:00

abstract：:We examined the effect of the pyridoxal 5'-phosphate (PLP) cofactor on the activity and stability of the psychrophilic alanine racemase, having a high catalytic activity at low temperature, from Bacillus psychrosaccharolyticus at high temperatures. The decrease in the enzyme activity at incubation temperatures over 40...

journal_title：FEMS microbiology letters

authors： Okubo Y,Yokoigawa K,Esaki N,Soda K,Misono H

更新日期：2000-11-15 00:00:00

abstract：:The denitrifying bacterium Thauera aromatica strain AR-1 grows anaerobically with protocatechuate (3,4-dihydroxybenzoate (DHB)) as sole energy and carbon source. This bacterium harbors two distinct pathways for degradation of aromatic compounds, the benzoyl-coenzyme A (CoA) pathway for benzoate degradation and the hyd...

journal_title：FEMS microbiology letters

authors： Philipp B,Kemmler D,Hellstern J,Gorny N,Caballero A,Schink B

更新日期：2002-06-18 00:00:00

abstract：:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed...

journal_title：FEMS microbiology letters

authors： Vierny C,Iaccarino M

更新日期：1989-07-01 00:00:00

abstract：:Improving the ability of plants and plant-associated organisms to fix and assimilate atmospheric nitrogen has inspired plant biotechnologists for decades, not only to alleviate negative effects on nature from increased use and availability of reactive nitrogen, but also because of apparent economic benefits and opport...

journal_title：FEMS microbiology letters

authors： Burén S,Rubio LM

更新日期：2018-02-01 00:00:00

abstract：:The molecular mechanisms controlling expression of the long polar fimbriae 2 (Lpf2) of enterohemorrhagic Escherichia coli (EHEC) O157:H7 were evaluated. Primer extension was used to locate the lpfA2 transcriptional start site in EHEC strain EDL933 at 171 bp upstream of the lpfA2 start codon. Semi-quantitative RT-PCR d...

journal_title：FEMS microbiology letters

authors： Arenas-Hernández MM,Rojas-López M,Medrano-López A,Nuñez-Reza KJ,Puente JL,Martínez-Laguna Y,Torres AG

更新日期：2014-08-01 00:00:00

abstract：:The influence of nutritional and physical stress on sporulation, conidial germination and vegetative biomass of Ophiocordyceps sinensis, one of the most important medicinal fungi in China and now globally, was evaluated using a two-stage culture method. All the treatments, except nutrient deprivation, enhanced conidia...

journal_title：FEMS microbiology letters

authors： Ren SY,Yao YJ

更新日期：2013-09-01 00:00:00

abstract：:The relationship between sugar availability and RTX (repeats in toxin) cytotoxin (leukotoxin) production in the periodontopathic bacterium, Actinobacillus actinomycetemcomitans, was investigated using a chemostat. A actinomycetemcomitans 301-b produced significant amounts of leukotoxin in anaerobic fructose-limited ch...

journal_title：FEMS microbiology letters

authors： Mizoguchi K,Ohta H,Miyagi A,Kurihara H,Takashiba S,Kato K,Murayama Y,Fukui K

更新日期：1997-01-01 00:00:00

abstract：:Microbial competition for carbon sources is a primary determinant of rhizosphere ecology. We employed the PCR to examine the population fluctuations of a symbiotic nitrogen-fixing bacterium (Rhizobium etli) during the first 11 days following inoculation of Phaseolus vulgaris seedlings grown in the presence or absence ...

journal_title：FEMS microbiology letters

authors： Petersen DJ,Srinivasan M,Chanway CP

更新日期：1996-09-01 00:00:00