Abstract:
:We examined the effect of the pyridoxal 5'-phosphate (PLP) cofactor on the activity and stability of the psychrophilic alanine racemase, having a high catalytic activity at low temperature, from Bacillus psychrosaccharolyticus at high temperatures. The decrease in the enzyme activity at incubation temperatures over 40 degrees C was consistent with the decrease in the amount of bound PLP. Unfolding of the enzyme at temperatures above 40 degrees C was suppressed in the presence of PLP. In the presence of 0.125 mM PLP, the specific activity of the psychrophilic enzyme was higher than that of a thermophilic alanine racemase, having a high catalytic activity at high temperature, from Bacillus stearothermophilus even at 60 degrees C.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Okubo Y,Yokoigawa K,Esaki N,Soda K,Misono Hdoi
10.1111/j.1574-6968.2000.tb09377.xkeywords:
subject
Has Abstractpub_date
2000-11-15 00:00:00pages
169-73issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(00)00427-4journal_volume
192pub_type
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