Abstract:
:Thermophilic bacteria have recently attracted great attention because of their potential application in improving different biochemical processes such as anaerobic digestion of various substrates, wastewater treatment or hydrogen production. In this study we report on the design of a specific 16S rRNA-targeted oligonucleotide probe for detecting members of Coprothermobacter genus characterized by a strong protease activity to degrade proteins and peptides. The newly designed CTH485 probe and helper probes hCTH429 and hCTH439 were optimized for use in fluorescence in situ hybridization (FISH) on thermophilic anaerobic sludge samples. In situ probing revealed that thermo-adaptive mechanisms shaping the 16S rRNA gene may affect the identification of thermophilic microorganisms. The novel developed FISH probe extends the possibility to study the widespread thermophilic syntrophic interaction of Coprothermobacter spp. with hydrogenotrophic methanogenic archaea, whose establishment is a great benefit for the whole anaerobic system.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Gagliano MC,Braguglia CM,Rossetti Sdoi
10.1111/1574-6968.12528subject
Has Abstractpub_date
2014-09-01 00:00:00pages
55-63issue
1eissn
0378-1097issn
1574-6968journal_volume
358pub_type
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