Abstract:
:The existence in our laboratory of over 10000 Salmonella typhimurium LT2 cultures sealed in agar stab vials for 33-46 years offers an opportunity for evolutionary and mutational studies. In each of 77 vials examined, 10(3)-10(5) colony forming units per vial were recovered (less than 0.01% of the original population) even after decades of undisturbed storage. Considerable genetic variability was observed in these populations. Three genetic variables, chromosome fragment size as determined by pulsed-field gel electrophoresis, extensive mutational reversions from nutritional auxotrophy to prototrophy, and differences in protein content as assayed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, were measured.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Edwards K,Linetsky I,Hueser C,Eisenstark Adoi
10.1111/j.1574-6968.2001.tb10677.xkeywords:
subject
Has Abstractpub_date
2001-05-30 00:00:00pages
215-9issue
2eissn
0378-1097issn
1574-6968pii
S0378109701001859journal_volume
199pub_type
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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journal_title:FEMS microbiology letters
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