Aerotactic responses in bacteria to photoreleased oxygen.

Abstract:

:Bacterial aerotaxis is a rapid response towards or away from oxygen. Here we report on the use of computer-assisted motion analysis coupled to flash photolysis of caged oxygen to quantify aerotactic responses in bacteria. The caged compound (mu-peroxo)(mu-hydroxo)bis[bis(bipyridyl) cobalt(III)] perchlorate liberates molecular oxygen upon irradiation with near-UV light. A mixture of cells and the caged oxygen compound was placed in a capillary tube and challenged by discrete stimuli of molecular oxygen produced by photolysis. We then recorded the rate of change of direction (rcd) as an estimate of tumble frequency in response to liberated oxygen and measured the signal processing (excitation) times in Bacillus subtilis, Bacillus halodurans and Escherichia coli. This computer-assisted caged oxygen assay gives a unique physiological profile of different aerotaxis transducers in bacteria.

journal_name

FEMS Microbiol Lett

authors

Yu HS,Saw JH,Hou S,Larsen RW,Watts KJ,Johnson MS,Zimmer MA,Ordal GW,Taylor BL,Alam M

doi

10.1111/j.1574-6968.2002.tb11481.x

keywords:

subject

Has Abstract

pub_date

2002-12-17 00:00:00

pages

237-42

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378109702010881

journal_volume

217

pub_type

杂志文章
  • Fine structure of hepatitis B virus surface antigen produced by recombinant yeast: comparison with HBsAg of human origin.

    abstract::The ultrastructure of hepatitis B virus surface antigen (HBsAg) particles produced by recombinant yeast cells was examined using high-resolution negative staining, and ice embedding, electron microscopy. With negative staining, the HBsAg particles were spherical to slightly ovoid with a mean diameter of 27.5 nm and co...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1998.tb13171.x

    authors: Yamaguchi M,Sugahara K,Shiosaki K,Mizokami H,Takeo K

    更新日期:1998-08-15 00:00:00

  • Efficient cloning in Bacillus megaterium: comparison to Bacillus subtilis and Escherichia coli cloning hosts.

    abstract::Quantitative cloning efficiencies for B. megaterium, B. subtilis, and E. coli were compared. Transformation of B. megaterium is less efficient than transformation of B. subtilis or E. coli. The frequency of recombinant clones was equal in E. coli and B. megaterium; both somewhat higher than in B. subtilis. Equivalent ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1990.tb13994.x

    authors: Von Tersch MA,Robbins HL

    更新日期:1990-08-01 00:00:00

  • Dr fimbriae coding region associated hemolytic activity of Escherichia coli.

    abstract::We investigated the hemolytic activity of Escherichia coli strain EC901 carrying plasmid pBJN406 containing genes draA-E involved in expression of the mannose-resistant Dr hemagglutinin, and in its isogenic insertion mutants devised with Tn5, Tn3, and TnphoA. While E. coli BN406 displayed rapid hemolytic activity agai...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(95)00177-7

    authors: Goluszko P,Nowicki S,Kaul AK,Pham T,Nowicki BJ

    更新日期:1995-07-15 00:00:00

  • Aerobic biodegradation of benzene and toluene under hypersaline conditions at the Great Salt Plains, Oklahoma.

    abstract::The Great Salt Plains is a 65-km(2) hypersaline habitat of geological origin located in north-central Oklahoma. Contamination of such ecosystems by petroleum compounds is expected from non-point sources and due to increased human activities. Little information exists about the ability of halophilic and halotolerant ba...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.03.014

    authors: Nicholson CA,Fathepure BZ

    更新日期:2005-04-15 00:00:00

  • Eight-hour PCR-based procedure for the detection of Salmonella in raw oysters.

    abstract::The aim of this study was to evaluate the ability of a nested PCR system to detect Salmonella senftenberg in raw oysters. The specific primers of the PCR were derived from the invA gene sequence, essential for Salmonella invasiveness into epithelial cells. First, for the extraction of DNA, four methods (guanidine isot...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.12.016

    authors: Vázquez-Novelle MD,Pazos AJ,Abad M,Sánchez JL,Pérez-Parallé ML

    更新日期:2005-02-01 00:00:00

  • Discovery of novel Vibrio cholerae VSP-II genomic islands using comparative genomic analysis.

    abstract::This report describes Vibrio seventh pandemic island II (VSP-II) and three novel variants revealed by comparative genomics of 23 Vibrio cholerae strains and their presence among a large and diverse collection of V. cholerae isolates. Three VSP-II variants were reported previously and our results demonstrate the presen...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02008.x

    authors: Taviani E,Grim CJ,Choi J,Chun J,Haley B,Hasan NA,Huq A,Colwell RR

    更新日期:2010-07-01 00:00:00

  • Surface Listeria monocytogenes carbohydrate-binding components revealed by agglutination with neoglycoproteins.

    abstract::Carbohydrate-binding components were shown to be present at the surface of Listeria monocytogenes by means of a panel of neoglycoproteins using direct agglutination. These lectin-like components bind on neoglycoproteins bearing D-glucosamine, L-fucosylamine, or para-amino-phenyl-alpha-D-mannopyrannoside residues. The ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/s0378-1097(05)80058-8

    authors: Cottin J,Loiseau O,Robert R,Mahaza C,Carbonnelle B,Senet JM

    更新日期:1990-03-15 00:00:00

  • Isolation of nuclei from Aspergillus nidulans protoplasts.

    abstract::Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifuga...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(91)90268-f

    authors: Vágvölgyi C,Ferenczy L

    更新日期:1991-08-15 00:00:00

  • Listeria monocytogenes infection of Caco-2 human epithelial cells induces activation of transcription factor NF-kappa B/Rel-like DNA binding activities.

    abstract::The effect of Listeria monocytogenes infection on the cellular level of transcription factor NF-kappa B in the human epithelia-like cell line Caco-2 was investigated. Infection with L. monocytogenes or treatment with lipoteichoic acid induced the formation of three NF-kappa B-like DNA-protein complexes C1, C2, and C3,...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb13766.x

    authors: Hauf N,Goebel W,Fiedler F,Kuhn M

    更新日期:1999-09-01 00:00:00

  • Occurrence of a lipase in spores of Alternaria brassicicola with a crucial role in the infection of cauliflower leaves.

    abstract::Alternaria brassicicola is a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80-kDa lipase (E.C 3.1.1. 3) was detected by SDS-PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross-reacted...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1999.tb08794.x

    authors: Berto P,Comménil P,Belingheri L,Dehorter B

    更新日期:1999-11-15 00:00:00

  • Characterization of extended-spectrum-beta-lactamase (CTX-M-15)-producing strains of Salmonella enterica isolated in France and Senegal.

    abstract::Three clinical isolates of Salmonella enterica recovered between 2000 and 2003 in France and Senegal were found to produce extended-spectrum beta-lactamase CTX-M-15. The two isolates from Senegal were recovered from stool of a hospitalized patient with gastroenteritis in 2000 and from an urine specimen of an out-patie...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.07.058

    authors: Weill FX,Perrier-Gros-Claude JD,Demartin M,Coignard S,Grimont PA

    更新日期:2004-09-15 00:00:00

  • Estimate of the diversity of viral and bacterial assemblage in the coastal water of Lake Baikal.

    abstract::In this study, we analysed the diversity and composition of double-stranded DNA viral and bacterial communities within the sample of surface coastal water of Southern Baikal through metagenomics and deep sequencing of the 16S ribosomal RNA gene, respectively. The 16S rRNA gene analysis has revealed 14 phyla and domina...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnz094

    authors: Butina TV,Bukin YS,Krasnopeev AS,Belykh OI,Tupikin AE,Kabilov MR,Sakirko МV,Belikov SI

    更新日期:2019-05-01 00:00:00

  • The bacteriocidal effects of transition metal complexes containing the NO+ group on the food-spoilage bacterium Clostridium sporogenes.

    abstract::The chemical and molecular mechanism of toxicity of nitrite towards food-spoilage bacteria such as Clostridium botulinum or Clostridium sporogenes is not well understood. In order to discover the active species and explore its chemistry, a number of compounds related to nitrite were synthesized. Their bacteriocidal ef...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(92)90133-9

    authors: Cui X,Joannou CL,Hughes MN,Cammack R

    更新日期:1992-11-01 00:00:00

  • Vibrioferrin production by the food spoilage bacterium Pseudomonas fragi.

    abstract::Pseudomonas fragi is a meat and milk spoilage bacterium with high iron requirements; however, mechanisms of iron acquisition remain largely unknown. The aim of this work was to investigate siderophore production as an iron acquisition system for P. fragi. A vibrioferrin siderophore gene cluster was identified in 13 P....

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx279

    authors: Stanborough T,Fegan N,Powell SM,Tamplin M,Chandry PS

    更新日期:2018-03-01 00:00:00

  • Marinitalea sucinacia gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment.

    abstract::An amber-pigmented, Gram-negative, rod-shaped and aerobic bacterial strain devoid of flagella, designated strain JC2131(T) , was isolated from tidal flat sediment of Dongmak in Ganghwa island, South Korea. Identification was carried out on the basis of polyphasic taxonomy. Phylogenetic analysis based on the 16S rRNA g...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02147.x

    authors: Kim KY,Park SJ,Hahm YT,Cha CJ

    更新日期:2011-01-01 00:00:00

  • An improved method for detecting cytostatic toxin (emetic toxin) of Bacillus cereus and its application to food samples.

    abstract::We developed an improved HEp-2 cell assay method for the detection of Bacillus cereus toxin, which affects the proliferation of HEp-2 cells. The cytostatic toxin was stable upon exposure to heat, pH 2, pH 11 and trypsin, which suggests it is an emetic. Using the HEp-2 cell assay, we examined the distribution and conta...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1994.tb06866.x

    authors: Mikami T,Horikawa T,Murakami T,Matsumoto T,Yamakawa A,Murayama S,Katagiri S,Shinagawa K,Suzuki M

    更新日期:1994-06-01 00:00:00

  • Extension of resistance to cefepime and cefpirome associated to a six amino acid deletion in the H-10 helix of the cephalosporinase of an Enterobacter cloacae clinical isolate.

    abstract::Enterobacter cloacae CHE, a clinical strain with overproduced cephalosporinase was found to be highly resistant to the new cephalosporins, cefepime and cefpirome (MICs> or =128 microg ml(-1)). The strain was isolated from a child previously treated with cefepime. The catalytic efficiency of the purified enzyme with th...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2001.tb10519.x

    authors: Barnaud G,Labia R,Raskine L,Sanson-Le Pors MJ,Philippon A,Arlet G

    更新日期:2001-02-20 00:00:00

  • Quantification of class 1 integron abundance in natural environments using real-time quantitative PCR.

    abstract::Integrons are bacterial genetic elements capable of capturing and expressing potentially adaptive genetic material. Class 1 integrons constitute the most intensely studied group of these elements to date, mainly due to their well-established role in the acquisition and dissemination of antibiotic resistance genes in c...

    journal_title:FEMS microbiology letters

    pub_type: 信件

    doi:10.1111/j.1574-6968.2007.00992.x

    authors: Hardwick SA,Stokes HW,Findlay S,Taylor M,Gillings MR

    更新日期:2008-01-01 00:00:00

  • Diversity of endophytic fungi of Paeonia lactiflora Pallas and screening for fungal paeoniflorin producers.

    abstract::Endophytic fungi from Paeonia lactiflora Pallas, which is mainly distributed in China, were characterized and screened to identify those capable of producing paeoniflorin. A total of 101 isolates obtained from the roots, stems and leaves of P. lactiflora were grouped into 16 fungal taxa based on morphological traits a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny263

    authors: Cheng X,Wei Z,Pu S,Xiang M,Yan A,Zhang Y,Wang X

    更新日期:2018-12-01 00:00:00

  • Induction of a viable but non-culturable state in Salmonella Typhimurium by thermosonication and factors affecting resuscitation.

    abstract::The objective of this work was to analyze the effects of thermosonication (TS) on induction of a viable but non-culturable (VBNC) state in Salmonella Typhimurium and to examine incubation factors affecting subsequent resuscitation of cells. A TS treatment of 380 W at 53°C for 30 min induced the VBNC state in S. Typhim...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnx249

    authors: Liao H,Jiang L,Zhang R

    更新日期:2018-02-01 00:00:00

  • The 70 kilodalton iron regulated protein of Neisseria meningitidis is not the human transferrin receptor.

    abstract::Neisseria meningitidis is able to chelate iron from human transferrin (HTF), the main sequestrator of extracellular iron in vivo. Previous workers have reported that a ca. 70 kilodalton (kDa) iron regulated outer membrane protein (FeRP-70) is a highly specific receptor for HTF. We have examined the interaction between...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/0378-1097(90)90409-j

    authors: Ala'Aldeen DA,Davies HA,Wall RA,Borriello SP

    更新日期:1990-05-01 00:00:00

  • Diversity of pigmented Gram-positive bacteria associated with marine macroalgae from Antarctica.

    abstract::Little is known about the diversity and roles of Gram-positive and pigmented bacteria in Antarctic environments, especially those associated with marine macroorganisms. This work is the first study about the diversity and antimicrobial activity of culturable pigmented Gram-positive bacteria associated with marine Anta...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fnv206

    authors: Leiva S,Alvarado P,Huang Y,Wang J,Garrido I

    更新日期:2015-12-01 00:00:00

  • The light-harvesting complex II (B800-850) of Rhodobacter sulfidophilus: characterization and formation under different growth conditions.

    abstract::The photosynthetic bacterium Rhodobacter sulfidophilus is able to grow chemotrophically and phototrophically at a broad range of light intensities. In contrast to other facultative phototrophs, R. sulfidophilus synthesizes reaction center and light-harvesting (LH) complexes, B870 (LHI) and B800-850 (LHII) even under f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.1995.tb07382.x

    authors: Hagemann GE,Gad'on N,Garcia A,Drews G,Tadros MH

    更新日期:1995-02-01 00:00:00

  • The Serratia-type hemolysin of Chromobacterium violaceum.

    abstract::Chromobacterium violaceum is a Gram-negative opportunistic human pathogen and an inhabitant of tropical soils and waterways. Although known primarily for the synthesis of the pigment violacein, and more recently as a reporter strain for quorum sensing, clinical reports of chromobacteriosis comprise the largest block o...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2006.00566.x

    authors: Brumbach KC,Eason BD,Anderson LK

    更新日期:2007-02-01 00:00:00

  • The arbuscular mycorrhizal fungus, Glomus irregulare, controls the mycotoxin production of Fusarium sambucinum in the pathogenesis of potato.

    abstract::Trichothecenes are an important family of mycotoxins produced by several species of the genus Fusarium. These fungi cause serious disease on infected plants and postharvest storage of crops, and the toxins can cause health problems for humans and animals. Unfortunately, there are few methods for controlling mycotoxin ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/1574-6968.12236

    authors: Ismail Y,McCormick S,Hijri M

    更新日期:2013-11-01 00:00:00

  • Molybdate-dependent expression of dimethylsulfoxide reductase in Rhodobacter capsulatus.

    abstract::Expression of the dimethylsulfoxide respiratory (dor) operon of Rhodobacter is regulated by oxygen, light intensity and availability of substrate. Since dimethylsulfoxide reductase contains a pterin molybdenum cofactor, the role of molybdate in the regulation of dor operon expression was investigated. In this report w...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2000.tb09287.x

    authors: Solomon PS,Shaw AL,Young MD,Leimkuhler S,Hanson GR,Klipp W,McEwan AG

    更新日期:2000-09-15 00:00:00

  • Proteomic analysis of the insoluble subproteome of Clostridium difficile strain 630.

    abstract::Clostridium difficile, a Gram-positive spore-forming anaerobe, causes infections in humans ranging from mild diarrhoeal to potentially life-threatening pseudomembranous colitis. The availability of genomic information for a range of C. difficile strains affords researchers the opportunity to better understand not only...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1111/j.1574-6968.2010.02111.x

    authors: Jain S,Graham RL,McMullan G,Ternan NG

    更新日期:2010-11-01 00:00:00

  • Spreading the message of antimicrobial resistance: a detailed account of a successful public engagement event.

    abstract::The increase in Antimicrobial resistance (AMR) microorganisms has been exacerbated by exposure to antimicrobial drugs (e.g. antibiotics). A solution to AMR may require academic researchers to not only contribute to the drug discovery pipeline through laboratory research, but also to engage and inform non-specialist au...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1093/femsle/fny175

    authors: Redfern J,Bowater L,Crossley M,Verran J

    更新日期:2018-08-01 00:00:00

  • Transcriptome analysis of the Mg2+-responsive PhoP regulator in Yersinia pestis.

    abstract::PhoP was previously shown to be important for Yersinia pestis survival in macrophage and under macrophage-induced stresses. In this work, a phoP disruptant of Y. pestis 201 was generated using the Red cloning procedure. The transcription profile of the wild-type Y. pestis was then compared with that of the phoP mutant...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2005.06.053

    authors: Zhou D,Han Y,Qin L,Chen Z,Qiu J,Song Y,Li B,Wang J,Guo Z,Du Z,Wang X,Yang R

    更新日期:2005-09-01 00:00:00

  • Role of Helicobacter pylori rfaJ genes (HP0159 and HP1416) in lipopolysaccharide synthesis.

    abstract::The genome of Helicobacter pylori 26695 has been sequenced and the lipopolysaccharide (LPS) O sidechain of this strain has been shown to express both Lewis x and Lewis y units. To determine the role of HP0159 and HP1416, genes recognized as rfaJ homologs and implicated in LPS synthesis, isogenic mutants of H. pylori 2...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章

    doi:10.1016/j.femsle.2004.10.004

    authors: Moran AP,Shiberu B,Ferris JA,Knirel YA,Senchenkova SN,Perepelov AV,Jansson PE,Goldberg JB

    更新日期:2004-12-01 00:00:00