Abstract:
:Bacterial aerotaxis is a rapid response towards or away from oxygen. Here we report on the use of computer-assisted motion analysis coupled to flash photolysis of caged oxygen to quantify aerotactic responses in bacteria. The caged compound (mu-peroxo)(mu-hydroxo)bis[bis(bipyridyl) cobalt(III)] perchlorate liberates molecular oxygen upon irradiation with near-UV light. A mixture of cells and the caged oxygen compound was placed in a capillary tube and challenged by discrete stimuli of molecular oxygen produced by photolysis. We then recorded the rate of change of direction (rcd) as an estimate of tumble frequency in response to liberated oxygen and measured the signal processing (excitation) times in Bacillus subtilis, Bacillus halodurans and Escherichia coli. This computer-assisted caged oxygen assay gives a unique physiological profile of different aerotaxis transducers in bacteria.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Yu HS,Saw JH,Hou S,Larsen RW,Watts KJ,Johnson MS,Zimmer MA,Ordal GW,Taylor BL,Alam Mdoi
10.1111/j.1574-6968.2002.tb11481.xkeywords:
subject
Has Abstractpub_date
2002-12-17 00:00:00pages
237-42issue
2eissn
0378-1097issn
1574-6968pii
S0378109702010881journal_volume
217pub_type
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