Abstract:
:Alternaria brassicicola is a pathogen that penetrates directly through the host cuticle thanks to several serine esterases, according to our findings. Among these, an 80-kDa lipase (E.C 3.1.1. 3) was detected by SDS-PAGE and immunoblotting in the water washings of ungerminated spores. The purified lipase cross-reacted with Botrytis cinerea anti-lipase antibodies, which were reported to inhibit the in vitro lipase activity. Anti-lipase antibodies were added to a conidial suspension of A. brassicicola prior to inoculation. As a result, blackspot lesions were reduced by 90% on intact cauliflower leaves, but not on leaves from which surface wax had been removed. Spore surface-bound lipase is thought to interact closely with epicuticular leaf waxes for adhesion and/or penetration of the fungal propagules during the early stages of host-parasite interactions.
journal_name
FEMS Microbiol Lettjournal_title
FEMS microbiology lettersauthors
Berto P,Comménil P,Belingheri L,Dehorter Bdoi
10.1111/j.1574-6968.1999.tb08794.xkeywords:
subject
Has Abstractpub_date
1999-11-15 00:00:00pages
183-9issue
2eissn
0378-1097issn
1574-6968pii
S0378-1097(99)00484-Xjournal_volume
180pub_type
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