Construction and transduction of a shuttle vector bearing the cos site of Streptomyces phage phi C31 and determination of its cohesive ends.


:A plasmid has been constructed which is a bifunctional vector for Escherichia coli and for streptomycetes, containing the cos site of Streptomyces phage phi C31. It can be efficiently transduced into S. lividans 66 and into several other Streptomyces species. The nucleotide sequence of a 311-bp DNA fragment containing the cohesive ends has been determined. The cohesive ends consist of 10 bases protruding at the 3'-end of the phage genome.


FEMS Microbiol Lett


Kobler L,Schwertfirm G,Schmieger H,Bolotin A,Sladkova I




Has Abstract


1991-03-01 00:00:00












  • Production of monoclonal antibodies against a hemagglutinin/protease of Vibrio cholerae non-01.

    abstract::Two hybridoma cell lines producing monoclonal antibodies (MAbs) against a hemagglutinin/protease (HA/P) from Vibrio cholerae non-01 were produced and characterized. The two MAbs contained the kappa light chain and were IgG1 type. They similarly neutralized HA/P protease activity derived from both V. cholerae non-01 an...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Honda T,Hata-Naka A,Lertpocasombat K,Miwatani T

    更新日期:1991-03-01 00:00:00

  • Analysis of the Spirochaeta aurantia flaA gene and transcript.

    abstract::The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a sigma 70-like promoter. The translational start is 31 base pairs after the start of the transcript, the open ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Parales J Jr,Greenberg EP

    更新日期:1993-02-01 00:00:00

  • Cloning of xylanase gene of Streptomyces flavogriseus in Escherichia coli and bacteriophage lambda-induced lysis for the release of cloned enzyme.

    abstract::The xylanase gene of Streptomyces flavogriseus was cloned in pUC8 plasmid and expressed in Escherichia coli lysogenic for lambda cI857. lambda-Induced lysis of E. coli at 42 degrees C allowed efficient release of cloned enzyme activity in extracellular environment. The xylanase gene was located in the 0.8-kb HindIII f...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Srivastava R,Ali SS,Srivastava BS

    更新日期:1991-03-01 00:00:00

  • The Pseudomonas aeruginosa RhlR-controlled aegerolysin RahU is a low-affinity rhamnolipid-binding protein.

    abstract::The opportunistic pathogen Pseudomonas aeruginosa uses quorum-sensing systems to regulate collective behaviour in response to the environment, by linking the expression of particular genes to population density. The quorum-sensing transcription factors LasR and RhlR and their cognate N-acyl-homoserine lactone (HSL) si...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Miklavič Š,Kogovšek P,Hodnik V,Korošec J,Kladnik A,Anderluh G,Gutierrez-Aguirre I,Maček P,Butala M

    更新日期:2015-05-01 00:00:00

  • A fungus-type beta-galactofuranan in the cultivated Trebouxia photobiont of the lichen Ramalina gracilis.

    abstract::A structural characterization of polysaccharides extracted from the aposymbiotically cultured photobiont of the lichen Ramalina gracilis was carried out in order to compare them with those previously found in the symbiotic thallus. The photobiont was isolated from thallus fragments, following the method of Yamamoto, a...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cordeiro LM,Carbonero ER,Sassaki GL,Reis RA,Stocker-Wörgötter E,Gorin PA,Iacomini M

    更新日期:2005-03-01 00:00:00

  • The influence of sigma factors and ribosomal recognition elements on heterologous expression of cyanobacterial gene clusters in Escherichia coli.

    abstract::Cyanobacterial natural products offer new possibilities for drugs and lead compounds but many factors can inhibit the production of sufficient yields for pharmaceutical processes. While Escherichia coli and Streptomyces sp. have been used as heterologous expression hosts to produce cyanobacterial natural products, the...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wells KN,Videau P,Nelson D,Eiting JE,Philmus B

    更新日期:2018-08-01 00:00:00

  • Measurement of Pseudomonas aeruginosa multidrug efflux pumps by quantitative real-time polymerase chain reaction.

    abstract::Multidrug efflux pumps contribute to multiple antibiotic resistance in Pseudomonas aeruginosa. Pump expression usually has been quantified by Western blotting. Quantitative real-time polymerase chain reaction has been developed to measure mRNA expression for genes of interest. Whether this method correlates with pump ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yoneda K,Chikumi H,Murata T,Gotoh N,Yamamoto H,Fujiwara H,Nishino T,Shimizu E

    更新日期:2005-02-01 00:00:00

  • Analysis of CcpA mutations defective in carbon catabolite repression in Bacillus megaterium.

    abstract::Five mutations in ccpA of Bacillus megaterium with impaired functions were analysed for carbon catabolite repression. The phenotypes support the hypothesis that CcpA assumes a PurR/LacI fold. The completely inactive mutants CcpA119GE and CcpA326am cause alterations which are incompatible with that fold. A mutation wit...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Kraus A,Hillen W

    更新日期:1997-08-01 00:00:00

  • Location of catalase in crystalline peroxisomes of methanol-grown Hansenula polymorpha.

    abstract::We have studied the intraperoxisomal location of catalase in peroxisomes of methanol-grown Hansenula polymorpha by (immuno)cytochemical means. In completely crystalline peroxisomes, in which the crystalline matrix is composed of octameric alcohol oxidase (AO) molecules, most of the catalase protein is located in a nar...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Keizer I,Roggenkamp R,Harder W,Veenhuis M

    更新日期:1992-05-15 00:00:00

  • Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.

    abstract::Organic acids such as monocarboxylic acids, dicarboxylic acids or even more complex molecules such as sugar acids, have displayed great applicability in the industry as these compounds are used as platform chemicals for polymer, food, agricultural and pharmaceutical sectors. Chemical synthesis of these compounds from ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Soares-Silva I,Ribas D,Sousa-Silva M,Azevedo-Silva J,Rendulić T,Casal M

    更新日期:2020-08-01 00:00:00

  • Co-expression of the Bordetella pertussis leader peptidase I results in enhanced processing and expression of the pertussis toxin S1 subunit in Escherichia coli.

    abstract::Bordetella pertussis is the causative agent of whooping cough. Traditional vaccines against this disease are inherently reactogenic, thus research is currently focussed on the production of less reactive, acellular vaccines. Expression of candidate antigens for these vaccines in Escherichia coli would be preferable, h...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Smith AM,Yan H,Groves N,Dalla Pozza T,Walker MJ

    更新日期:2000-10-15 00:00:00

  • Most influential FEMS publications.

    abstract::A selection of influential FEMS publications to celebrate the 40th anniversary of FEMS. ...

    journal_title:FEMS microbiology letters

    pub_type: 历史文章,杂志文章


    authors: Prosser JI,Cole JA,Nielsen J,Bavoil PM,Häggblom MM

    更新日期:2014-05-01 00:00:00

  • Electrofusion of fragile mutants of Saccharomyces cerevisiae.

    abstract::This communication presents experimental evidence that intact fragile (osmotic sensitive) yeasts can be electrofused and give viable hybrids. The yield increases with one order of magnitude for electrofusion of intact fragile yeasts with protoplasts of non-fragile ones. The yield of viable hybrids, obtained by electro...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Tsoneva I,Doinov P,Dimitrov DS

    更新日期:1989-07-01 00:00:00

  • DNA sequencing reveals bacterial communities in midgut and other parts of the larvae of Spodoptera exigua Hubner (Lepidoptera: Noctuidae).

    abstract::Gut microbiota has been suggested as an important component of host defense. However, microbiota in other parts of the body have rarely been characterized. In our present work, we give a detailed view of the body of Spodoptera exigua larvae, the midgut with intestinal contents (MG), midgut excluding intestinal content...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Gao X,Li W,Luo J,Zhang L,Ji J,Zhu X,Wang L,Zhang K,Zhang S,Cui J

    更新日期:2020-02-01 00:00:00

  • Occurrence of aminoglycoside-modifying enzymes among isolates of Escherichia coli exhibiting high levels of aminoglycoside resistance isolated from Korean cattle farms.

    abstract::This study investigated 247 Escherichia coli isolates collected from four cattle farms to characterize aminoglycoside-modifying enzyme (AME) genes, their plasmid replicons and transferability. Out of 247 isolates a high number of isolates (total 202; 81.78%) were found to be resistant to various antibiotics by disc di...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Belaynehe KM,Shin SW,Hong-Tae P,Yoo HS

    更新日期:2017-08-01 00:00:00

  • Use of a lux reporter system for monitoring rapid changes in alpha-toxin gene expression in Clostridium perfringens during growth.

    abstract::To determine whether the luxA-luxB reporter system is suitable as a sensitive reporter for rapid real-time measurements of alpha-toxin gene (cpa) expression in Clostridium perfringens, and to widen the range of alpha-toxin-producing C. perfringens strains examined with respect to cpa expression during growth, the repo...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Phillips-Jones MK

    更新日期:2000-07-01 00:00:00

  • Beyond the single answer: a process-oriented exam for science students.

    abstract::This commentary describes an assessment exercise known as the TRIPSE (Tri-Partite Problem Solving Exercise) that mimics science in operation. Students frame hypotheses based on limited data, design experiments to test them, which they later revise with new information. It is emphasised that there are no single correct...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Nastos S,Rangachari PK

    更新日期:2016-08-01 00:00:00

  • Evidence for xylooligosaccharide utilization in Weissella strains isolated from Indian fermented foods and vegetables.

    abstract::Six strains isolated from fermented food were identified as Weissella species by 16S rDNA sequencing, clustering with the species pair W. confusa/W. cibaria. The strains were analysed for growth on glucose, xylose and xylooligosaccharides (XOS). All strains were xylose positive using the API CHL 50 test. Growth on XOS...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Patel A,Falck P,Shah N,Immerzeel P,Adlercreutz P,Stålbrand H,Prajapati JB,Holst O,Nordberg Karlsson E

    更新日期:2013-09-01 00:00:00

  • H4 histone in the macronucleus of Blepharisma japonicum (Protozoa, Ciliophora, Heterotrichida).

    abstract::Two clones, obtained by polymerase chain reaction from macronuclear DNA of the unicellular ciliated protist Blepharisma japonicum, were isolated and sequenced. They correspond to fragments of two different putative H4 histone genes. The existence of multiple H4 histone genes was also suggested by Southern blot hybridi...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Salvini M,Bini E,Santucci A,Batistoni R

    更新日期:1997-04-01 00:00:00

  • Helicobacter pylori strain ATCC700392 encodes a methyl-accepting chemotaxis receptor protein (MCP) for arginine and sodium bicarbonate.

    abstract::Helicobacter pylori ATCC43504 responds chemotactically to aspartic acid and serine, but not to arginine, nor to sodium bicarbonate. In contrast, H. pylori ATCC700392 (strain 26695) shows chemotaxis to all four attractants. Open reading frame HP0099 from H. pylori 26695 is predicted to encode one of three methyl-accept...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Cerda O,Rivas A,Toledo H

    更新日期:2003-07-29 00:00:00

  • A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis.

    abstract::A multiplex-polymerase chain reaction (PCR) assay based on one-step amplification and detection of two different mycobacterial genomic fragments was designed for differentiation of Mycobacterium bovis and Mycobacterium tuberculosis. The oligonucleotide primers were chosen from a 500-bp genomic fragment which is well c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shah DH,Verma R,Bakshi CS,Singh RK

    更新日期:2002-08-27 00:00:00

  • Construction of Escherichia coli-Arthrobacter-Rhodococcus shuttle vectors based on a cryptic plasmid from Arthrobacter rhombi and investigation of their application for functional screening.

    abstract::A cryptic plasmid from Arthrobacter rhombi PRH1, designated as pPRH, was sequenced and characterized. It was 5000 bp in length with a G+C content of 66 mol%. The plasmid pPRH was predicted to encode six putative open reading frames (ORFs), in which ORF2 and ORF3 formed the minimal replicon of plasmid pPRH and shared 5...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Stanislauskiene R,Gasparaviciute R,Vaitekunas J,Meskiene R,Rutkiene R,Casaite V,Meskys R

    更新日期:2012-02-01 00:00:00

  • The effects of mutation of the anr gene on the aerobic respiratory chain of Pseudomonas aeruginosa.

    abstract::The anr gene of Pseudomonas aeruginosa encodes a transcriptional regulator of anaerobic gene expression, homologous to the Fnr protein of Escherichia coli. We report here that Anr has a role in regulating the activity of the aerobic respiratory chain of P. aeruginosa. Strains with internal deletions in their anr gene ...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Ray A,Williams HD

    更新日期:1997-11-15 00:00:00

  • Gene encoding a replication initiator protein and replication origin of conjugative plasmid pSA1.1 of Streptomyces cyaneus ATCC 14921.

    abstract::pSA1.1 is a 9.1-kb multicopy plasmid originally isolated from Streptomyces cyaneus (formerly S. azureus) ATCC 14921. This plasmid accumulates single-stranded DNA in S. lividans and is therefore considered to replicate by a rolling-circle replication. In the present work, the rep gene encoding the replication initiator...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Yokoyama E,Matsuzaki Y,Doi K,Ogata S

    更新日期:1998-12-01 00:00:00

  • Ultra-violet resonance Raman spectroscopy for the rapid discrimination of urinary tract infection bacteria.

    abstract::The ability to identify pathogenic organisms rapidly provides significant benefits to clinicians; in particular, with respect to best prescription practices and tracking of recurrent infections. Conventional bioassays require 3-5 days before identification of an organism can be made, thus compromising the effectivenes...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Jarvis RM,Goodacre R

    更新日期:2004-03-19 00:00:00

  • Infection by the 'photosynthetic' phage S-PM2 induces increased synthesis of phycoerythrin in Synechococcus sp. WH7803.

    abstract::Phycoerythrin-containing Synechococcus strains are unicellular cyanobacteria that are of great ecological importance in the marine environment. These organisms are known to be susceptible to infection by cyanophages (viruses that infect cyanobacteria). The infection cycle takes several hours and during this time the c...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Shan J,Jia Y,Clokie MR,Mann NH

    更新日期:2008-06-01 00:00:00

  • Genomic heterogeneity in Chlorobium limicola: chromosomic and plasmidic differences among strains.

    abstract::Chromosome analysis by pulsed-field gel electrophoresis shows a high level of genetic heterogeneity between the two subspecies of the green sulfur bacteria Chlorobium limicola analysed: C. limicola and C. limicola f. s. thiosulfatophilum. Currently, they are differentiated only by the ability to utilize thiosulfate as...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Méndez-Alvarez S,Pavoń V,Esteve I,Guerrero R,Gaju N

    更新日期:1995-12-15 00:00:00

  • Segmented filamentous bacteria in human ileostomy samples after high-fiber intake.

    abstract::Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects ...

    journal_title:FEMS microbiology letters

    pub_type: 信件


    authors: Jonsson H

    更新日期:2013-05-01 00:00:00

  • Changes in fatty acid composition associated with differentiation of Trypanosoma cruzi.

    abstract::The fatty acid composition during the transformation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes (metacyclogenesis) was analysed by gas-liquid chromatography and mass spectrometry. Significant qualitative and quantitative changes in the fatty acid composition occurred during incubation of epimas...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Esteves MG,Gonzales-Perdomo M,Alviano CS,Angluster J,Goldenberg S

    更新日期:1989-05-01 00:00:00

  • Bioinformatic and genetic characterization of three genes localized adjacent to the major replication origin of Haloferax volcanii.

    abstract::In haloarchaea, a cluster of three genes is localized directly adjacent to the major replication origin, and, hence, the encoded proteins were annotated as 'origin-associated proteins' (Oap). However, prior to this study, no experimental data were available for these conserved hypothetical proteins. Bioinformatic anal...

    journal_title:FEMS microbiology letters

    pub_type: 杂志文章


    authors: Wolters M,Borst A,Pfeiffer F,Soppa J

    更新日期:2019-11-01 00:00:00