Bioluminescence-based system for rapid detection of natural transformation.

abstract：:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed...

journal_title：FEMS microbiology letters

authors： Vierny C,Iaccarino M

更新日期：1989-07-01 00:00:00

abstract：:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from t...

journal_title：FEMS microbiology letters

authors： Brans A,Loriaux A,Thamm I,Joris B,Dusart J

更新日期：1997-04-15 00:00:00

abstract：:A compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from Dowex 1 x 8 extracts of culture supernatants of Vibrio parahaemolyticus AQ 3354. The strain synthesized the compound in culture media containing less than 2 microM added FeCl3. Hydrolysis of the compou...

journal_title：FEMS microbiology letters

authors： Yamamoto S,Fujita Y,Okujo N,Minami C,Matsuura S,Shinoda S

更新日期：1992-07-01 00:00:00

abstract：:A particular property of Clostridium difficile strain C34 is an insertion of approximately 2 kb in the tcdA-C34 gene that does not hinder expression of a fully active TcdA-C34 molecule. Intoxication with TcdA-C34 induced an arborized appearance in eukaryotic cells (D-type cytopathic effect); intoxication with TcdB-C34...

journal_title：FEMS microbiology letters

authors： Mehlig M,Moos M,Braun V,Kalt B,Mahony DE,von Eichel-Streiber C

更新日期：2001-05-01 00:00:00

abstract：:The Bacteroides fragilis enterotoxin is an extracellular zinc metalloprotease that has been implicated in diarrheal disease of humans and animals. This toxin causes fluid accumulation in intestinal loops and is cytotoxic for HT-29 cells, an intestinal carcinoma cell line. Here we report the cloning and sequencing of t...

journal_title：FEMS microbiology letters

authors： Kling JJ,Wright RL,Moncrief JS,Wilkins TD

更新日期：1997-01-15 00:00:00

abstract：:The pseudotrisaccharide allosamidin, a potent inhibitor of chitinases, retarded the fragmentation of hyphae but did not affect the fungal growth and cephalosporin-C production in Acremonium chrysogenum. In vitro inhibition of A. chrysogenum cell-bound chitinase(s) by allosamidin revealed that about 47% of the soluble ...

journal_title：FEMS microbiology letters

authors： Sándor E,Pusztahelyi T,Karaffa L,Karányi Z,Pócsi I,Biró S,Szentirmai A,Pócsi I

更新日期：1998-07-15 00:00:00

abstract：:A continuous culture technique utilising a variant of Staphylococcus aureus 8325-4 containing transcriptional gene fusions was used to investigate the relationships between cell density (OD(600)), steady-state specific growth rate (mu) and expression of both agr (accessory gene regulator) and tst (toxic shock syndrome...

journal_title：FEMS microbiology letters

authors： Wright JD,Holland KT

更新日期：2003-01-28 00:00:00

abstract：:Pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. Computer analysis of the P. ananatis SC17(0) sequenced genome revealed an ORF encoding a homologue (named gcd) of the mGDH (EC 1.1.99.17) apoenzyme from Escherichia coli and a putative pyrroloquinoline quinone (PQQ) biosynthetic o...

journal_title：FEMS microbiology letters

authors： Andreeva IG,Golubeva LI,Kuvaeva TM,Gak ER,Katashkina JI,Mashko SV

更新日期：2011-05-01 00:00:00

abstract：:Chronological analysis of 125 Vibrio cholerae O139 strains isolated during 1993-2005 in Kolkata revealed the prevalence of two new genotypes of cholera toxin (CT) and novel combinations of ctxB and rstR alleles resulting in variant CTX prophages. One of the new genotypes of ctxB, which first appeared in 1996 with the ...

journal_title：FEMS microbiology letters

authors： Raychoudhuri A,Mukherjee P,Ramamurthy T,Nandy RK,Takeda Y,Nair GB,Mukhopadhyay AK

更新日期：2010-02-01 00:00:00

abstract：:The effect feeding antibiotics has on the bacterial population of the rabbit caecum was investigated. No changes in total volatile fatty acid production or total bacterial counts were observed compared with nonantibiotic treated controls. However, treatment with chlortetracycline resulted in an increase of propionate ...

journal_title：FEMS microbiology letters

authors： Abecia L,Fondevila M,Balcells J,Edwards JE,Newbold CJ,McEwan NR

更新日期：2007-07-01 00:00:00

abstract：:The polyhydroxyalkanoic acid (PHA) synthase gene (phaCAc) of a species of Acinetobacter isolated from an activated sludge treatment plant was cloned by heterologous complementation in a poly-beta-hydroxybutyrate (PHB) negative mutant of Alcaligenes eutrophus. Nucleotide sequence analysis of phaCAc revealed an open rea...

journal_title：FEMS microbiology letters

authors： Schembri MA,Bayly RC,Davies JK

更新日期：1994-05-01 00:00:00

abstract：:In addition to core Cas proteins, CRISPR-Cas loci often encode ancillary proteins that modulate the activity of the respective effectors in interference. Subtype VI-B1 CRISPR-Cas systems encode the Csx27 protein that down-regulates the activity of Cas13b when the type VI-B locus is expressed in Escherichia coli. We sh...

journal_title：FEMS microbiology letters

authors： Makarova KS,Gao L,Zhang F,Koonin EV

更新日期：2019-04-01 00:00:00

abstract：:A Phoma sp. was isolated and characterized as endophytic and as a pathogen of Larrea tridentata (creosote bush) growing in the desert region of southern Utah, USA. This fungus produces a unique mixture of volatile organic compounds (VOCs), including a series of sesquiterpenoids, some alcohols and several reduced napht...

journal_title：FEMS microbiology letters

authors： Strobel G,Singh SK,Riyaz-Ul-Hassan S,Mitchell AM,Geary B,Sears J

更新日期：2011-07-01 00:00:00

abstract：:A novel cry1A was cloned from Bacillus thuringiensis strain BT8 and expressed in the B. thuringiensis acrystalliferous mutant HD73(-). The gene, designated cry1Ah1, encoded a protein with a molecular weight of 134 kDa. Reverse transcriptase-PCR and Western blotting showed that Cry1Ah was expressed in the host strain B...

journal_title：FEMS microbiology letters

authors： Xue J,Liang G,Crickmore N,Li H,He K,Song F,Feng X,Huang D,Zhang J

更新日期：2008-03-01 00:00:00

abstract：:We isolated nickel-resistant bacterium from soil in order to identify a novel nickel resistance determinant. Using 16S rRNA gene sequencing, an isolate was identified as Enterobacter sp. Ni15. This species showed a medium-level (resistant to up to 10 mM) nickel resistance in nutrient-rich media. Enterobacter sp. Ni15 ...

journal_title：FEMS microbiology letters

authors： Lee YK,Chang HH,Lee HJ,Park H,Lee KH,Joe MH

更新日期：2006-04-01 00:00:00

abstract：:The number and incidence of Collinsella aerofaciens in the human intestine are the highest among Gram-positive non-spore-forming bacilli. Identification of this species is very difficult and requires considerable time. A PCR-based identification system using C. aerofaciens-specific primers is described. Using this PCR...

journal_title：FEMS microbiology letters

authors： Kageyama A,Sakamoto M,Benno Y

更新日期：2000-02-01 00:00:00

abstract：:The asexual spore, or conidium, is critical in the life cycle of many fungi because it is the primary means for dispersion and serves as a 'safe house' for the fungal genome in adverse environmental conditions. This review discusses the physiological process of germination, conidial adhesion and initiation of protein ...

journal_title：FEMS microbiology letters

authors： Osherov N,May GS

更新日期：2001-05-30 00:00:00

abstract：:The beta-toxin gene from Clostridium perfringens type C was cloned and expressed as a glutathione S-transferase fusion protein in Escherichia coli. The DNA sequence was determined and compared to the type B sequence. Two nucleotide differences were found in the protein coding sequence, resulting in one amino acid diff...

journal_title：FEMS microbiology letters

authors： Steinthorsdottir V,Fridriksdottir V,Gunnarsson E,Andrésson OS

更新日期：1995-08-01 00:00:00

abstract：:Cry4Ba is a delta-endotoxin produced by Bacillus thuringiensis subsp. israelensis and Cyt2Aa2 is a cytolytic delta-endotoxin produced by B. thuringiensis subsp. darmstadiensis. Cry4Ba produced in Escherichia coli was toxic to Aedes aegypti larvae (LC(50)=140 ng ml(-1)) but virtually inactive to Culex quinquefasciatus ...

journal_title：FEMS microbiology letters

authors： Promdonkoy B,Promdonkoy P,Panyim S

更新日期：2005-11-01 00:00:00

abstract：:Two actinomycete strains, CHR3 and CHR28, were isolated from metal-contaminated sediments from Baltimore Inner Harbor. The isolates were classified as Streptomyces spp. Agar diffusion assays showed both isolates to be resistant to mercuric chloride and phenylmercuric acetate. Hybridization experiments indicated that g...

journal_title：FEMS microbiology letters

authors： Ravel J,Amoroso MJ,Colwell RR,Hill RT

更新日期：1998-05-01 00:00:00

abstract：:Glutamate uptake in the Gram-positive Corynebacterium glutamicum is mediated via a binding protein-dependent transport system, which is encoded by the gluABCD gene cluster. Cloning of these genes in an expression vector and subsequent transformation of the resulting plasmid allows different strains of the Gram-negativ...

journal_title：FEMS microbiology letters

authors： Burkovski A,Krämer R

更新日期：1995-04-01 00:00:00

abstract：:Saccharomyces cerevisiae genes encoding functions necessary for inhibition by the Pseudomonas syringae pv. syringae cyclic lipodepsipeptide, syringomycin-E, were identified by mutant analyses. Syringomycin-E-resistant mutants were isolated, shown to contain single recessive mutations, and divided into eight gene compl...

journal_title：FEMS microbiology letters

authors： Takemoto JY,Yu Y,Stock SD,Miyakawa T

更新日期：1993-12-15 00:00:00

abstract：:Cell-free protein synthesis has emerged in recent years as a powerful tool that can potentially transform the production of recombinant proteins. Cell-free protein synthesis harnesses the synthetic power of living cells while eliminating many of the constraints of traditional cell-based gene expression methods. Due to...

journal_title：FEMS microbiology letters

authors： Lee KH,Kim DM

更新日期：2018-09-01 00:00:00

abstract：:A lysC-lac'Z fusion plasmid was constructed to study the regulatory region of the lysC gene. Analysis by deletion mutations confirmed the existence of an alternative promoter, P2, located upstream of the previously identified promoter, P1. The transcription start site of promoter P2 was located 85 base pairs upstream ...

journal_title：FEMS microbiology letters

authors： Liao HH,Hseu TH

更新日期：1998-11-01 00:00:00

abstract：:The applicability of different PCR-based techniques, random amplified polymorphic DNA, cleaved amplified polymorphic sequence and SSRs (simple sequence repeats) to genetically identify, typify and discriminate among Saccharomyces cerevisiae strains, was investigated. The analysis and comparison of the genetic profiles...

journal_title：FEMS microbiology letters

authors： Pérez MA,Gallego FJ,Hidalgo P

更新日期：2001-12-18 00:00:00

abstract：:FlgM inhibits the flagella-specific sigma factor FliA and is involved in the mouse-virulence of Salmonella typhimurium. In recent experiments, we observed that: (i) a flgM gene that could function to negatively regulate flagella synthesis was present in a variety of salmonellae; and (ii) the flgM gene derived from Sal...

journal_title：FEMS microbiology letters

authors： Schmitt CK,Darnell SC,O'Brien AD

更新日期：1996-01-15 00:00:00

abstract：:Endopolygalacturonases (endoPGs) of some phytopathogens are virulent factors for dicots. To investigate the function of the endoPG of Magnaporthe oryzae, a disruption mutant of MGG_08938, the homolog of endoPG found in the genome database of this fungus, was generated. The pathogenicity, mycelial growth, and appressor...

journal_title：FEMS microbiology letters

authors： Mori T,Jung HY,Maejima K,Hirata H,Himeno M,Hamamoto H,Namba S

更新日期：2008-03-01 00:00:00

abstract：:The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The resu...

journal_title：FEMS microbiology letters

authors： Malke H,Mechold U,Gase K,Gerlach D

更新日期：1994-02-01 00:00:00

abstract：:The enzymic interconversion of Escherichia coli isocitrate dehydrogenase (ICDH) between the catalytically active and inactive forms is mediated through the activities of ICDH-kinase/phosphatase in response to changes in the metabolic environment. In this study, the use of mutant strains devoid of isocitrate lyase (ace...

journal_title：FEMS microbiology letters

authors： el-Mansi EM

更新日期：1998-09-15 00:00:00

abstract：:Clostridium thermocellum, a Gram-positive, thermophilic anaerobe produces a highly active cellulase system. This system, termed the cellulosome, is a complex composed of at least 14-18 different types of components organized around a large, cellulose-binding protein. Combining recombinant DNA technology and protein bi...

journal_title：FEMS microbiology letters

authors： Béguin P,Millet J,Aubert JP

更新日期：1992-12-15 00:00:00