Use of an automatic DNA sequencer for S1 mapping: transcriptional analysis of the Streptomyces coelicolor A3(2) dnaK operon.

Abstract:

:The transcription start point of the dnaK operon of Streptomyces coelicolor A3(2) has been determined by S1 mapping, using the EMBL automated fluorescent DNA sequencer. The -35 and -10 hexamers correspond to a sigma 70-type promoter. This promoter responds to heat shock and involves an inverted repeat different from the CIRCE sequence characteristic of the Gram-positive heat-shock promoters.

journal_name

FEMS Microbiol Lett

authors

Brans A,Loriaux A,Thamm I,Joris B,Dusart J

doi

10.1111/j.1574-6968.1997.tb10327.x

subject

Has Abstract

pub_date

1997-04-15 00:00:00

pages

189-94

issue

2

eissn

0378-1097

issn

1574-6968

pii

S0378-1097(97)00074-8

journal_volume

149

pub_type

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