Evaluation of molecular techniques for the genetic characterization of Saccharomyces cerevisiae strains.

abstract：:Lyme disease is a multisystemic disorder caused by Borrelia burgdorferi, an invasive spirochete. B. burgdorferi has a predilection for collagenous tissue and one major clinical manifestation of the disease is arthritis. We have identified a collagenolytic activity in B. burgdorferi detergent lysates using iodinated ge...

journal_title：FEMS microbiology letters

authors： Grab DJ,Kennedy R,Philipp MT

更新日期：1996-10-15 00:00:00

abstract：:The ADP-ribosylation factor (ARF) family of GTPases are highly conserved from yeast to human and regulate vesicle budding. Sec7 domain containing proteins stimulate the guanine nucleotide exchange on Arf proteins, while ARF-GTPase activating proteins stimulate the hydrolysis of GTP. Since vesicle trafficking is import...

journal_title：FEMS microbiology letters

authors： Oscarsson T,Walther A,Lengeler KB,Wendland J

更新日期：2017-12-29 00:00:00

abstract：:We determined the complete nucleotide sequence of the 16341 bp plasmid pHH205 of the extremely halophilic archaeon Halobacterium salinarum J7. The plasmid has a G+C content of 61.1%. A number of direct and inverted repeat sequences were found in pHH205, while no insertion sequences were found. Thirty-eight large open ...

journal_title：FEMS microbiology letters

authors： Ye X,Ou J,Ni L,Shi W,Shen P

更新日期：2003-04-11 00:00:00

abstract：:Pectinases are enzymes that catalyze pectin degradation. There is a global demand for pectinases because of their wide utility and catalytic efficiency. Optimization of the fermentation process to increase the pectolytic enzyme activity is generally practiced to lower process costs, but whether temperature influences ...

journal_title：FEMS microbiology letters

authors： Guan Y,Yin D,Du X,Ye X

更新日期：2018-12-01 00:00:00

abstract：:A total of 401 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates from two experimentally infected calves were analyzed using molecular biological methods. Genetic differences detected by pulsed-field gel electrophoresis were observed between the inoculated and recovered strains as early as 1 day post inoculat...

journal_title：FEMS microbiology letters

authors： Akiba M,Sameshima T,Nakazawa M

更新日期：2000-03-01 00:00:00

abstract：:The streptokinase gene of Streptococcus equisimilis H46 was inactivated by plasmid insertion mutagenesis to study the relationship between elaboration of streptokinase and acquisition of cell-associated plasmin activity after incubation of wild-type and mutant cells in media containing plasminogen or plasmin. The resu...

journal_title：FEMS microbiology letters

authors： Malke H,Mechold U,Gase K,Gerlach D

更新日期：1994-02-01 00:00:00

abstract：:Cytoplasmic precursors of the peptidoglycan biosynthetic pathway were purified from vancomycin-treated, glycopeptide-sensitive and -resistant strains of Enterococcus faecium. Resistance was due to production of a modified precursor, UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, where lactate was identified on the basi...

journal_title：FEMS microbiology letters

authors： Messer J,Reynolds PE

更新日期：1992-07-01 00:00:00

abstract：:This communication presents experimental evidence that intact fragile (osmotic sensitive) yeasts can be electrofused and give viable hybrids. The yield increases with one order of magnitude for electrofusion of intact fragile yeasts with protoplasts of non-fragile ones. The yield of viable hybrids, obtained by electro...

journal_title：FEMS microbiology letters

authors： Tsoneva I,Doinov P,Dimitrov DS

更新日期：1989-07-01 00:00:00

abstract：:We have investigated the specificity of six different lysozymes for peptidoglycan substrates obtained by extraction of a number of gram-negative bacteria and Micrococcus lysodeikticus with chloroform/Tris-HCl buffer (chloroform/buffer). The lysozymes included two that are commercially available (hen egg white lysozyme...

journal_title：FEMS microbiology letters

authors： Nakimbugwe D,Masschalck B,Deckers D,Callewaert L,Aertsen A,Michiels CW

更新日期：2006-06-01 00:00:00

abstract：:Development of pili on cells of Arthrobacter photogonimos is induced by photo-oxidative conditions. The nucleotide sequence was determined of a light-inducible gene (lipA) that encodes the precursor of a light-inducible pilin (designated LIP), a polypeptide of 212 amino acids. The N-terminal leader peptide includes a ...

journal_title：FEMS microbiology letters

authors： Yang HS,Hoober JK

更新日期：1998-06-01 00:00:00

abstract：:The RcsCDB (Rcs) phosphorelay system regulates capsule synthesis, flagella production and other cellular activities in several enteric bacteria. This system consists of three proteins: the sensor RcsC, the cognate response regulator RcsB and the histidine-containing phosphotransfer protein RcsD (YojN), which is hypoth...

journal_title：FEMS microbiology letters

authors： Pescaretti Mde L,Morero R,Delgado MA

更新日期：2009-11-01 00:00:00

abstract：:Xylose reductase catalyzes the NAD(P)H-dependent reduction of xylose to xylitol and is essential for growth on xylose by yeasts. To understand the nature of coenzyme binding to the Pichia stipitis xylose reductase, we investigated the role of the strictly conserved Lys270 in the putative IPKS coenzyme binding motif by...

journal_title：FEMS microbiology letters

authors： Kostrzynska M,Sopher CR,Lee H

更新日期：1998-02-01 00:00:00

abstract：:In this study, we were interested in the association of attenuated mutants of Salmonella enterica serovar Enteritidis with subpopulations of porcine white blood cells (WBC). The mutants included those with inactivated aroA, phoP, rfaL, rfaG, rfaC and fliC genes and a mutant with five major pathogenicity islands remove...

journal_title：FEMS microbiology letters

authors： Stepanova H,Volf J,Malcova M,Matiasovic J,Faldyna M,Rychlik I

更新日期：2011-08-01 00:00:00

abstract：:To study the pathogenicity of mouse cytomegalovirus (MCMV) and to identify virulence determinants, we have isolated and phenotypically characterised 25 temperature-sensitive (ts) mutants. Six of these (tsm9, tsm13, tsm20, tsm22, tsm28 and tsm30) failed to replicate in mice and were avirulent. Five mutants (tsm14, tsm1...

journal_title：FEMS microbiology letters

authors： Akel HM,Sweet C

更新日期：1993-11-01 00:00:00

abstract：:A new type II restriction endonuclease, SshAI, was purified from Salmonella shikmonah TK139 of kangaroo origin. The recognition and cleavage specificity of Ssh AI was determined to be 5'-CC/TNAGG-3', identical to that of SauI from Streptomyces aureofaciens and Bsu36I from Bacillus subtilis. Based on closely related an...

journal_title：FEMS microbiology letters

authors： Miyahara M,Nakajima K,Kawanishi T,Mise K

更新日期：1990-01-01 00:00:00

abstract：:The wine yeast Saccharomyces cerevisiae is central in the production of aroma compounds during fermentation. Some of the most important yeast-derived aroma compounds produced are esters. The esters ethyl acetate and isoamyl acetate are formed from alcohols and acetyl-CoA in a reaction catalysed by alcohol acetyltransf...

journal_title：FEMS microbiology letters

authors： Cordente AG,Swiegers JH,Hegardt FG,Pretorius IS

更新日期：2007-02-01 00:00:00

abstract：:A new Volvariella volvacea gene encoding a carbohydrate esterase (CE) family 4 acetyl xylan esterase (AXE) (designated as VvaxeII) was cloned and characterized. The coded polypeptide had 253 amino acid residues, with the first 19 serving as a secretion signal peptide. The VvaxeII transcript levels were high when the f...

journal_title：FEMS microbiology letters

authors： Liu X,Ding S

更新日期：2009-06-01 00:00:00

abstract：:Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between...

journal_title：FEMS microbiology letters

authors： Cateau E,Verdon J,Fernandez B,Hechard Y,Rodier MH

更新日期：2011-06-01 00:00:00

abstract：:The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and successfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into Mycobacterium smeg...

journal_title：FEMS microbiology letters

authors： Lazraq R,Clavel-Sérès S,David HL,Roulland-Dussoix D

更新日期：1990-05-01 00:00:00

abstract：:Bacteria carry a number of genes that cause cell growth arrest or cell lysis upon expression. Notably, defective prophages retain many lysis proteins. Here, we identified a novel lytic gene, ydfD, on the Qin prophage segment of the Escherichia coli genome. YdfD lyses 99.9% of cells within 2 h of its induction. The co-...

journal_title：FEMS microbiology letters

authors： Masuda H,Awano N,Inouye M

更新日期：2016-03-01 00:00:00

abstract：:An internal segment of the penicillin-binding protein gene, pbpA, of Streptomyces griseus was amplified from genomic DNA using the polymerase chain reaction and used as a hybridization probe to isolate the complete gene from a cosmid library. pbpA encodes a 485 amino acid sequence that conserves three motifs of PBPs, ...

journal_title：FEMS microbiology letters

authors： Jiang H,Kendrick KE

更新日期：2000-12-01 00:00:00

abstract：:A chemiluminescence immunoassay (CLIA) was developed to detect Cryptosporidium parvum growth in Madin-Darby canine kidney (MDCK) cell cultures. Optimal results were obtained when MDCK cells were plated at a density of 1 x 10(4) cells/well (96-well plate) and maintained as a monolayer for 4 days prior to infection with...

journal_title：FEMS microbiology letters

authors： You X,Arrowood MJ,Lejkowski M,Xie L,Schinazi RF,Mead JR

更新日期：1996-03-01 00:00:00

abstract：:Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-charact...

journal_title：FEMS microbiology letters

authors： Vos M,Sibleyras L,Lo LK,Hesse E,Gaze W,Klümper U

更新日期：2020-02-01 00:00:00

abstract：:We recently described adhesion to and invasion of bovine mammary gland cells by Staphylococcus aureus in vitro. Here, we show that the levels of adhesion and invasion are dependent on the bacterial growth phase and are controlled by the agr locus. Incubation of exponential growth phase cells of S. aureus with mammary ...

journal_title：FEMS microbiology letters

authors： Lammers A,Nuijten PJ,Smith HE

更新日期：1999-11-01 00:00:00

abstract：:The extensively discussed idea of oxidative stress development under antibiotic treatment was confirmed using an antioxidant gene expression (soxRS-, oxyR-regulon) approach, including microaerobic cultivation conditions. The killing action of antibiotics and their ability to cause peroxide oxidative stress in Escheric...

journal_title：FEMS microbiology letters

authors： Akhova AV,Tkachenko AG

更新日期：2014-04-01 00:00:00

abstract：:beta-Glucosidase in Aspergillus nidulans was found to be both intracellular and extracellular. The intracellular beta-glucosidase was synthesized after the exhaustion of carbon source in the medium. The extracellular enzyme appeared with autolysis of the mycelium. Biosynthesis of beta-glucosidase was not induced by va...

journal_title：FEMS microbiology letters

authors： Lee J,Kwon KS,Hah YC

更新日期：1996-01-01 00:00:00

abstract：:Mathematical modeling is an important tool in biological research, allowing for the synthesis of results from many studies into an understanding of a system. Despite this, the need for extensive subject matter knowledge and complex mathematics often leaves modeling as an esoteric subspecialty. A 2-fold approach can be...

journal_title：FEMS microbiology letters

authors： Lofgren ET

更新日期：2016-10-01 00:00:00

abstract：:Trichoderma reesei is a filamentous fungus widely used as an efficient protein producer and known to secrete large quantities of biomass degrading enzymes. Much work has been done aimed at improving the secretion efficiency of this fungus. It is generally accepted that the major bottlenecks in secretion are protein fo...

journal_title：FEMS microbiology letters

authors： Diener SE,Dunn-Coleman N,Foreman P,Houfek TD,Teunissen PJ,van Solingen P,Dankmeyer L,Mitchell TK,Ward M,Dean RA

更新日期：2004-01-30 00:00:00

abstract：:The application of series of high intensity electric pulses to a yeast suspension provoked a considerable release of some cytoplasmic proteins, glutathione reductase, 3-phosphoglycerate kinase and alcohol dehydrogenase. A maximal yield was achieved 3-8 h after pulsation. The electro-induced protein efflux was accelera...

journal_title：FEMS microbiology letters

authors： Ganeva V,Galutzov B

更新日期：1999-05-15 00:00:00

abstract：:Sugar alcohols (polyols) have important roles as nutrients, anti-freezing agents and scavengers of free radicals in cold-adapted bacteria, but the characteristics of polyol dehydrogenases in cold-adapted bacteria remain largely unknown. In this study, based on the observation that a cold-adapted bacterium Pseudomonas ...

journal_title：FEMS microbiology letters

authors： DangThu Q,Nguyen TT,Jang SH,Lee C

更新日期：2021-01-05 00:00:00